S22A7_RAT
ID S22A7_RAT Reviewed; 535 AA.
AC Q5RLM2; Q63314;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Solute carrier family 22 member 7;
DE AltName: Full=Novel liver transporter;
DE AltName: Full=Organic anion transporter 2;
GN Name=Slc22a7; Synonyms=Nlt, Oat2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8056831; DOI=10.1242/jcs.107.4.1065;
RA Simonson G.D., Vincent A.C., Roberg K.J., Huang Y., Iwanij V.;
RT "Molecular cloning and characterization of a novel liver-specific transport
RT protein.";
RL J. Cell Sci. 107:1065-1072(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RA Bourgea J., White E.P., Patten C.J., Crespi C.L., Xiao G.;
RT "A novel rat organic anion transporter 2.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9650585; DOI=10.1016/s0014-5793(98)00585-7;
RA Sekine T., Cha S.H., Tsuda M., Apiwattanakul N., Nakajima N., Kanai Y.,
RA Endou H.;
RT "Identification of multispecific organic anion transporter 2 expressed
RT predominantly in the liver.";
RL FEBS Lett. 429:179-182(1998).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RX PubMed=11504818;
RA Morita N., Kusuhara H., Sekine T., Endou H., Sugiyama Y.;
RT "Functional characterization of rat organic anion transporter 2 in LLC-PK1
RT cells.";
RL J. Pharmacol. Exp. Ther. 298:1179-1184(2001).
RN [5]
RP FUNCTION.
RX PubMed=15900017; DOI=10.1124/dmd.104.001552;
RA Morita N., Kusuhara H., Nozaki Y., Endou H., Sugiyama Y.;
RT "Functional involvement of rat organic anion transporter 2 (Slc22a7) in the
RT hepatic uptake of the nonsteroidal anti-inflammatory drug ketoprofen.";
RL Drug Metab. Dispos. 33:1151-1157(2005).
CC -!- FUNCTION: Mediates sodium-independent multispecific organic anion
CC transport. Transport of ketoprofen, indomethacin, salicylate,
CC acetylsalicylate, prostaglandin E2, 3'-azido-3'-deoxythymidine (AZT),
CC 2',3'-dideoxycytidine (ddC), dicarboxylates and p-aminohippurate (PAH).
CC {ECO:0000269|PubMed:11504818, ECO:0000269|PubMed:15900017,
CC ECO:0000269|PubMed:9650585}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.37 uM for indomethacin {ECO:0000269|PubMed:11504818};
CC KM=26 uM for AZT {ECO:0000269|PubMed:11504818};
CC KM=3.08 mM for ddC {ECO:0000269|PubMed:11504818};
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:11504818}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11504818}. Note=Apical side of the renal tubule.
CC -!- TISSUE SPECIFICITY: Expressed in liver and kidney.
CC {ECO:0000269|PubMed:9650585}.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
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DR EMBL; L27651; AAA57157.1; -; mRNA.
DR EMBL; AY816233; AAV66454.1; -; mRNA.
DR RefSeq; NP_445989.2; NM_053537.2.
DR AlphaFoldDB; Q5RLM2; -.
DR SMR; Q5RLM2; -.
DR STRING; 10116.ENSRNOP00000025094; -.
DR BindingDB; Q5RLM2; -.
DR ChEMBL; CHEMBL2073671; -.
DR TCDB; 2.A.1.19.15; the major facilitator superfamily (mfs).
DR PaxDb; Q5RLM2; -.
DR PRIDE; Q5RLM2; -.
DR Ensembl; ENSRNOT00000025094; ENSRNOP00000025094; ENSRNOG00000018420.
DR GeneID; 89776; -.
DR KEGG; rno:89776; -.
DR UCSC; RGD:621699; rat.
DR CTD; 10864; -.
DR RGD; 621699; Slc22a7.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000154922; -.
DR HOGENOM; CLU_001265_33_3_1; -.
DR InParanoid; Q5RLM2; -.
DR OMA; FPMETRA; -.
DR OrthoDB; 435631at2759; -.
DR PhylomeDB; Q5RLM2; -.
DR TreeFam; TF315847; -.
DR Reactome; R-RNO-561048; Organic anion transport.
DR Reactome; R-RNO-9749641; Aspirin ADME.
DR PRO; PR:Q5RLM2; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000018420; Expressed in liver and 6 other tissues.
DR Genevisible; Q5RLM2; RN.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:RGD.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015711; P:organic anion transport; IDA:RGD.
DR GO; GO:0035634; P:response to stilbenoid; ISO:RGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004749; Orgcat_transp/SVOP.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00898; 2A0119; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..535
FT /note="Solute carrier family 22 member 7"
FT /id="PRO_0000317486"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..509
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 479
FT /note="P -> R (in Ref. 1; AAA57157)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 535 AA; 58654 MW; E7C38969DCAE2B73 CRC64;
MGFEDLLDKV GGFGPFQLRN LVLMALPRML LPMHFLLPVF MAAVPAHHCA LPGAPANLSH
QDLWLEAHLP RETDGSFSSC LRFAYPQTVP NVTLGTEVSN SGEPEGEPLT VPCSQGWEYD
RSEFSSTIAT EWDLVCQQRG LNKITSTCFF IGVLVGAVVY GYLSDRFGRR RLLLVAYVSS
LVLGLMSAAS INYIMFVVTR TLTGSALAGF TIIVLPLELE WLDVEHRTVA GVISTVFWSG
GVLLLALVGY LIRSWRWLLL AATLPCVPGI ISIWWVPESA RWLLTQGRVE EAKKYLLSCA
KLNGRPVGEG SLSQEALNNV VTMERALQRP SYLDLFRTSQ LRHISLCCMM VWFGVNFSYY
GLTLDVSGLG LNVYQTQLLF GAVELPSKIM VYFLVRRLGR RLTEAGMLLG AALTFGTSLL
VSLETKSWIT ALVVVGKAFS EAAFTTAYLF TSELYPTVLR QTGLGLTALM GRLGASLAPL
AALLDGVWLL LPKVAYGGIA LVAACTALLL PETKKAQLPE TIQDVERKST QEEDV
