RUP2_ARATH
ID RUP2_ARATH Reviewed; 368 AA.
AC Q9FFA7; Q94BR3;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=WD repeat-containing protein RUP2;
DE AltName: Full=Protein EARLY FLOWERING BY OVEREXPRESSION 2;
DE AltName: Full=Protein REPRESSOR OF UV-B PHOTOMORPHOGENESIS 2;
GN Name=RUP2; Synonyms=EFO2; OrderedLocusNames=At5g23730; ORFNames=MRO11.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 12-368.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 126-368.
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH UVR8, SUBCELLULAR LOCATION, AND INDUCTION BY
RP UV-B.
RC STRAIN=cv. Columbia;
RX PubMed=21041653; DOI=10.1073/pnas.0914532107;
RA Gruber H., Heijde M., Heller W., Albert A., Seidlitz H.K., Ulm R.;
RT "Negative feedback regulation of UV-B-induced photomorphogenesis and stress
RT acclimation in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:20132-20137(2010).
RN [6]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=21242318; DOI=10.1093/jxb/erq336;
RA Wang W., Yang D., Feldmann K.A.;
RT "EFO1 and EFO2, encoding putative WD-domain proteins, have overlapping and
RT distinct roles in the regulation of vegetative development and flowering of
RT Arabidopsis.";
RL J. Exp. Bot. 62:1077-1088(2011).
RN [7]
RP INTERACTION WITH UVR8.
RX PubMed=22988111; DOI=10.1073/pnas.1210898109;
RA Cloix C., Kaiserli E., Heilmann M., Baxter K.J., Brown B.A., O'Hara A.,
RA Smith B.O., Christie J.M., Jenkins G.I.;
RT "C-terminal region of the UV-B photoreceptor UVR8 initiates signaling
RT through interaction with the COP1 protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:16366-16370(2012).
CC -!- FUNCTION: Functions in association with RUP1 as repressor of UV-B-
CC induced photomorphogenesis mediated by UVR8 and HY5. Plays a crucial
CC negative feedback regulatory role downstream of UVR8-COP1 to inhibit
CC UVR8 function, balance UV-B-specific responses and ensure normal plant
CC growth. Is involved in the regulation of photoperiodic flowering and
CC vegetative development. May act as negative regulator of photoperiodic
CC flowering by suppressing flowering through the action of CONSTANS (CO)
CC and FLOWERING LOCUS T (FT). {ECO:0000269|PubMed:21041653,
CC ECO:0000269|PubMed:21242318}.
CC -!- SUBUNIT: Interacts with UVR8. {ECO:0000269|PubMed:21041653,
CC ECO:0000269|PubMed:22988111}.
CC -!- INTERACTION:
CC Q9FFA7; Q9FN03: UVR8; NbExp=3; IntAct=EBI-15889073, EBI-2407499;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21041653}. Cytoplasm,
CC cytosol {ECO:0000269|PubMed:21041653}.
CC -!- INDUCTION: Circadian-regulation. Expression increases during the dark
CC phase with a peak at the beginning of the light phase and then
CC decreases to reach the lowest expression at the end of the light phase.
CC Induced by UV-B. {ECO:0000269|PubMed:21041653,
CC ECO:0000269|PubMed:21242318}.
CC -!- DISRUPTION PHENOTYPE: Small stunted leaves and early flowering under
CC short days conditions. {ECO:0000269|PubMed:21242318}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK64045.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB005244; BAB10046.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93205.1; -; Genomic_DNA.
DR EMBL; AY039941; AAK64045.1; ALT_INIT; mRNA.
DR EMBL; BT015119; AAT71991.1; -; mRNA.
DR RefSeq; NP_568435.2; NM_122278.4.
DR AlphaFoldDB; Q9FFA7; -.
DR SMR; Q9FFA7; -.
DR BioGRID; 17713; 1.
DR DIP; DIP-60745N; -.
DR IntAct; Q9FFA7; 1.
DR STRING; 3702.AT5G23730.1; -.
DR PaxDb; Q9FFA7; -.
DR PRIDE; Q9FFA7; -.
DR ProteomicsDB; 226658; -.
DR EnsemblPlants; AT5G23730.1; AT5G23730.1; AT5G23730.
DR GeneID; 832438; -.
DR Gramene; AT5G23730.1; AT5G23730.1; AT5G23730.
DR KEGG; ath:AT5G23730; -.
DR Araport; AT5G23730; -.
DR TAIR; locus:2172883; AT5G23730.
DR eggNOG; ENOG502QVDX; Eukaryota.
DR HOGENOM; CLU_006994_0_2_1; -.
DR InParanoid; Q9FFA7; -.
DR OMA; FTTHRRL; -.
DR OrthoDB; 857220at2759; -.
DR PhylomeDB; Q9FFA7; -.
DR PRO; PR:Q9FFA7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFA7; baseline and differential.
DR Genevisible; Q9FFA7; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0043254; P:regulation of protein-containing complex assembly; IDA:TAIR.
DR GO; GO:0010224; P:response to UV-B; IGI:TAIR.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR044616; RUP1/2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR45389; PTHR45389; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Flowering; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..368
FT /note="WD repeat-containing protein RUP2"
FT /id="PRO_0000421721"
FT REPEAT 38..77
FT /note="WD 1"
FT REPEAT 97..138
FT /note="WD 2"
FT REPEAT 141..184
FT /note="WD 3"
FT REPEAT 192..232
FT /note="WD 4"
FT REPEAT 236..276
FT /note="WD 5"
FT REPEAT 279..318
FT /note="WD 6"
FT REPEAT 330..368
FT /note="WD 7"
SQ SEQUENCE 368 AA; 40002 MW; 3860809FFF0C358C CRC64;
MNTLHPHKQQ QEQAQQQEEA RYEWDLSLST VVSSSSSSAS DVIGAIEFDP TDNIVATAGI
SRKIRFYGLP SLLRNNAVSG TGVSFVDQAT ACEYYICTPA KLSSLRWRPG SGGRVIGSGD
YDGVVMEYDL EKRTPVFERD EHGGRRVWSV DYTRHGGAST VGASGSDDGT MQVWDPRCPP
EESVGVVRPA GICRSAVCCV EFDPSGGPAV AVGCADRKGY VYDIRKLVDP ALTLQGHTKT
VSYVRFLDGG TVVTAGTDGC LKLWSVEDGR VIRTYEGHVN NRNFVGLSVW RNGALFGCGS
ENNRVFVYDR RWGKPVWVDG FEPVGMNSGS DKRFVSSVCW RQSGVDQCTL VAGGSDGVLQ
VYVGKRKP
