RSME_BACSU
ID RSME_BACSU Reviewed; 256 AA.
AC P54461;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase E;
DE EC=2.1.1.193;
DE AltName: Full=16S rRNA m3U1498 methyltransferase;
GN Name=rsmE; Synonyms=yqeU; OrderedLocusNames=BSU25440;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=9023197; DOI=10.1128/jb.179.4.1153-1164.1997;
RA Homuth G., Masuda S., Mogk A., Kobayashi Y., Schumann W.;
RT "The dnaK operon of Bacillus subtilis is heptacistronic.";
RL J. Bacteriol. 179:1153-1164(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 2-256.
RX PubMed=16021622; DOI=10.1002/prot.20541;
RA Badger J., Sauder J.M., Adams J.M., Antonysamy S., Bain K., Bergseid M.G.,
RA Buchanan S.G., Buchanan M.D., Batiyenko Y., Christopher J.A., Emtage S.,
RA Eroshkina A., Feil I., Furlong E.B., Gajiwala K.S., Gao X., He D.,
RA Hendle J., Huber A., Hoda K., Kearins P., Kissinger C., Laubert B.,
RA Lewis H.A., Lin J., Loomis K., Lorimer D., Louie G., Maletic M.,
RA Marsh C.D., Miller I., Molinari J., Muller-Dieckmann H.J., Newman J.M.,
RA Noland B.W., Pagarigan B., Park F., Peat T.S., Post K.W., Radojicic S.,
RA Ramos A., Romero R., Rutter M.E., Sanderson W.E., Schwinn K.D., Tresser J.,
RA Winhoven J., Wright T.A., Wu L., Xu J., Harris T.J.R.;
RT "Structural analysis of a set of proteins resulting from a bacterial
RT genomics project.";
RL Proteins 60:787-796(2005).
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D84432; BAA12467.1; -; Genomic_DNA.
DR EMBL; D83717; BAA12079.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14486.1; -; Genomic_DNA.
DR PIR; D69952; D69952.
DR RefSeq; NP_390422.1; NC_000964.3.
DR RefSeq; WP_003230015.1; NZ_JNCM01000036.1.
DR PDB; 1VHK; X-ray; 2.60 A; A/B/C/D=2-256.
DR PDBsum; 1VHK; -.
DR AlphaFoldDB; P54461; -.
DR SMR; P54461; -.
DR STRING; 224308.BSU25440; -.
DR PaxDb; P54461; -.
DR PRIDE; P54461; -.
DR EnsemblBacteria; CAB14486; CAB14486; BSU_25440.
DR GeneID; 937856; -.
DR KEGG; bsu:BSU25440; -.
DR PATRIC; fig|224308.179.peg.2765; -.
DR eggNOG; COG1385; Bacteria.
DR InParanoid; P54461; -.
DR OMA; TQWKGER; -.
DR PhylomeDB; P54461; -.
DR BioCyc; BSUB:BSU25440-MON; -.
DR EvolutionaryTrace; P54461; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070042; F:rRNA (uridine-N3-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0070475; P:rRNA base methylation; IBA:GO_Central.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; rRNA_ssu_MeTrfase-E.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR PANTHER; PTHR30027; PTHR30027; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR PIRSF; PIRSF015601; MTase_slr0722; 1.
DR SUPFAM; SSF75217; SSF75217; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00046; TIGR00046; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Methyltransferase; Reference proteome;
KW rRNA processing; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..256
FT /note="Ribosomal RNA small subunit methyltransferase E"
FT /id="PRO_0000176205"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 10..14
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 15..23
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 24..30
FT /evidence="ECO:0007829|PDB:1VHK"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 40..44
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 50..58
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 60..70
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 78..86
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 92..102
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 107..111
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 122..127
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 129..142
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 157..163
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 164..166
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 167..173
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 185..191
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 198..203
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 211..219
FT /evidence="ECO:0007829|PDB:1VHK"
FT STRAND 223..225
FT /evidence="ECO:0007829|PDB:1VHK"
FT TURN 234..236
FT /evidence="ECO:0007829|PDB:1VHK"
FT HELIX 237..250
FT /evidence="ECO:0007829|PDB:1VHK"
SQ SEQUENCE 256 AA; 28802 MW; AAE708FE4283157E CRC64;
MQRYFIELTK QQIEEAPTFS ITGEEVHHIV NVMRMNEGDQ IICCSQDGFE AKCELQSVSK
DKVSCLVIEW TNENRELPIK VYIASGLPKG DKLEWIIQKG TELGAHAFIP FQAARSVVKL
DDKKAKKKRE RWTKIAKEAA EQSYRNEVPR VMDVHSFQQL LQRMQDFDKC VVAYEESSKQ
GEISAFSAIV SSLPKGSSLL IVFGPEGGLT EAEVERLTEQ DGVTCGLGPR ILRTETAPLY
ALSAISYQTE LLRGDQ
