AUGN_MOUSE
ID AUGN_MOUSE Reviewed; 148 AA.
AC Q99LS0; Q9D2U7;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Augurin {ECO:0000305};
DE AltName: Full=Esophageal cancer-related gene 4 protein homolog {ECO:0000303|PubMed:20404145, ECO:0000303|PubMed:21349154};
DE Flags: Precursor;
GN Name=Ecrg4 {ECO:0000303|PubMed:20404145, ECO:0000303|PubMed:21349154};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Hippocampus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND CLEAVAGE
RP OF PROPEPTIDE AFTER ARG-70.
RX PubMed=17284679; DOI=10.1101/gr.5755407;
RA Mirabeau O., Perlas E., Severini C., Audero E., Gascuel O., Possenti R.,
RA Birney E., Rosenthal N., Gross C.;
RT "Identification of novel peptide hormones in the human proteome by hidden
RT Markov model screening.";
RL Genome Res. 17:320-327(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=20404145; DOI=10.1073/pnas.0911446107;
RA Kujuro Y., Suzuki N., Kondo T.;
RT "Esophageal cancer-related gene 4 is a secreted inducer of cell senescence
RT expressed by aged CNS precursor cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:8259-8264(2010).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=21349154; DOI=10.1186/2045-8118-8-6;
RA Gonzalez A.M., Podvin S., Lin S.Y., Miller M.C., Botfield H.,
RA Leadbeater W.E., Roberton A., Dang X., Knowling S.E., Cardenas-Galindo E.,
RA Donahue J.E., Stopa E.G., Johanson C.E., Coimbra R., Eliceiri B.P.,
RA Baird A.;
RT "Ecrg4 expression and its product augurin in the choroid plexus: impact on
RT fetal brain development, cerebrospinal fluid homeostasis and
RT neuroprogenitor cell response to CNS injury.";
RL Fluids Barriers CNS 8:6-6(2011).
CC -!- FUNCTION: Probable hormone that may attenuate cell proliferation and
CC induce senescence of oligodendrocyte and neural precursor cells in the
CC central nervous system (PubMed:20404145). ECRG4-induced senescence is
CC characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1
CC and CCND3 proteasomal degradation (PubMed:20404145).
CC {ECO:0000269|PubMed:20404145}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17284679,
CC ECO:0000269|PubMed:20404145}. Cytoplasm {ECO:0000250|UniProtKB:Q9H1Z8}.
CC Apical cell membrane {ECO:0000250|UniProtKB:Q9H1Z8}.
CC -!- TISSUE SPECIFICITY: Expressed in the intermediate lobe of pituitary,
CC glomerular layer of adrenal cortex, choroid plexus and atrioventricular
CC node of the heart (PubMed:17284679). Expressed in the brain with high
CC expression in the choroid plexus and the epithelial lining of the
CC central canal and expression in the gray matter of the spinal cord (at
CC protein level) (PubMed:21349154). {ECO:0000269|PubMed:17284679,
CC ECO:0000269|PubMed:21349154}.
CC -!- DEVELOPMENTAL STAGE: At embryonic stage 14.5 dpc, primarily expressed
CC in the choroid plexus, and low expression in the heart and cartilage
CC (at protein level) (PubMed:21349154). At 18.5 dpc, expressed in adrenal
CC cortex, choroid plexus and bone (PubMed:17284679). Low expression in
CC the brain of young, 2-month-old animals, except for the mitral cell
CC layer of the olfactory bulb (PubMed:20404145). Strongly expressed in
CC the brains of aged, 15- to 21-month-old mice with expression in the
CC subgranular zone of the dentate gyrus and in the corpus callosum (at
CC protein level) (PubMed:20404145). Also expressed in the CA1-3 regions
CC of the hippocampus, as well as in the cerebellum, brainstem and cortex
CC (PubMed:20404145). In vitro, up-regulated in senescent cells, including
CC embryonic fibroblasts and oligodendrocyte precursor cells
CC (PubMed:20404145). {ECO:0000269|PubMed:17284679,
CC ECO:0000269|PubMed:20404145, ECO:0000269|PubMed:21349154}.
CC -!- SIMILARITY: Belongs to the augurin family. {ECO:0000305}.
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DR EMBL; AK018770; BAB31398.1; -; mRNA.
DR EMBL; AK049805; BAE20668.1; -; mRNA.
DR EMBL; AC159092; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC002254; AAH02254.1; -; mRNA.
DR CCDS; CCDS14925.1; -.
DR RefSeq; NP_077245.2; NM_024283.3.
DR AlphaFoldDB; Q99LS0; -.
DR STRING; 10090.ENSMUSP00000027217; -.
DR PhosphoSitePlus; Q99LS0; -.
DR PaxDb; Q99LS0; -.
DR PRIDE; Q99LS0; -.
DR ProteomicsDB; 277136; -.
DR Antibodypedia; 2376; 226 antibodies from 28 providers.
DR DNASU; 78896; -.
DR Ensembl; ENSMUST00000027217; ENSMUSP00000027217; ENSMUSG00000026051.
DR GeneID; 78896; -.
DR KEGG; mmu:78896; -.
DR UCSC; uc007avp.2; mouse.
DR CTD; 84417; -.
DR MGI; MGI:1926146; Ecrg4.
DR VEuPathDB; HostDB:ENSMUSG00000026051; -.
DR eggNOG; ENOG502RZPP; Eukaryota.
DR GeneTree; ENSGT00390000000145; -.
DR HOGENOM; CLU_153579_0_0_1; -.
DR InParanoid; Q99LS0; -.
DR OMA; RNGHDYD; -.
DR OrthoDB; 1431738at2759; -.
DR TreeFam; TF336161; -.
DR BioGRID-ORCS; 78896; 1 hit in 72 CRISPR screens.
DR ChiTaRS; 1500015O10Rik; mouse.
DR PRO; PR:Q99LS0; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q99LS0; protein.
DR Bgee; ENSMUSG00000026051; Expressed in epithelium of cochlear duct and 132 other tissues.
DR Genevisible; Q99LS0; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031045; C:dense core granule; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISO:MGI.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IDA:MGI.
DR GO; GO:0090398; P:cellular senescence; IDA:MGI.
DR GO; GO:0007417; P:central nervous system development; ISS:UniProtKB.
DR GO; GO:0070314; P:G1 to G0 transition; IDA:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISO:MGI.
DR GO; GO:2000854; P:positive regulation of corticosterone secretion; ISO:MGI.
DR GO; GO:0051461; P:positive regulation of corticotropin secretion; ISO:MGI.
DR GO; GO:0051466; P:positive regulation of corticotropin-releasing hormone secretion; ISO:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0009611; P:response to wounding; ISS:UniProtKB.
DR GO; GO:0030103; P:vasopressin secretion; ISO:MGI.
DR InterPro; IPR028173; Augurin.
DR PANTHER; PTHR31613; PTHR31613; 1.
DR Pfam; PF15187; Augurin; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cleavage on pair of basic residues; Cytoplasm; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000250|UniProtKB:D4A540"
FT PROPEP 32..70
FT /evidence="ECO:0000305|PubMed:17284679"
FT /id="PRO_0000363239"
FT PEPTIDE 71..132
FT /note="Augurin"
FT /id="PRO_0000250515"
FT PROPEP 133..148
FT /evidence="ECO:0000255"
FT /id="PRO_0000363240"
FT CONFLICT 146
FT /note="N -> D (in Ref. 1; AAH02254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 148 AA; 16992 MW; A5C73BF987E0693B CRC64;
MSTSSARPAV LALAGLALLL LLCLGPDGIS GNKLKKMLQK REGPVPSKTN VAVAENTAKE
FLGGLKRAKR QLWDRTRPEV QQWYQQFLYM GFDEAKFEDD VNYWLNRNRN GHDYYGDYYQ
RHYDEDAAIG PHSRESFRHG ASVNYNDY
