AUG8_ARATH
ID AUG8_ARATH Reviewed; 644 AA.
AC Q9SUH5; F4JQE1;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=AUGMIN subunit 8 {ECO:0000303|PubMed:22505726};
DE AltName: Full=QWRF motif-containing protein 8 {ECO:0000303|PubMed:20978221};
DE AltName: Full=ROP1 enhancer 2 {ECO:0000303|PubMed:23735294};
GN Name=AUG8 {ECO:0000303|PubMed:22505726};
GN Synonyms=QWRF8 {ECO:0000303|PubMed:20978221},
GN REN2 {ECO:0000303|PubMed:23735294};
GN OrderedLocusNames=At4g30710 {ECO:0000312|Araport:AT4G30710};
GN ORFNames=T10C21.60 {ECO:0000312|EMBL:CAB52444.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20978221; DOI=10.1105/tpc.110.074781;
RA Albrecht V., Simkova K., Carrie C., Delannoy E., Giraud E., Whelan J.,
RA Small I.D., Apel K., Badger M.R., Pogson B.J.;
RT "The cytoskeleton and the peroxisomal-targeted snowy cotyledon3 protein are
RT required for chloroplast development in Arabidopsis.";
RL Plant Cell 22:3423-3438(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND IDENTIFICATION IN THE AUGMIN COMPLEX BY
RP MASS SPECTROMETRY.
RX PubMed=22505726; DOI=10.1105/tpc.112.096610;
RA Hotta T., Kong Z., Ho C.M., Zeng C.J., Horio T., Fong S., Vuong T.,
RA Lee Y.R., Liu B.;
RT "Characterization of the Arabidopsis augmin complex uncovers its critical
RT function in the assembly of the acentrosomal spindle and phragmoplast
RT microtubule arrays.";
RL Plant Cell 24:1494-1509(2012).
RN [7]
RP RETRACTED PAPER.
RX PubMed=23735294; DOI=10.1105/tpc.113.113472;
RA Cao L., Wang L., Zheng M., Cao H., Ding L., Zhang X., Fu Y.;
RT "Arabidopsis AUGMIN subunit8 is a microtubule plus-end binding protein that
RT promotes microtubule reorientation in hypocotyls.";
RL Plant Cell 25:2187-2201(2013).
RN [8]
RP RETRACTION NOTICE OF PUBMED:23735294.
RX PubMed=28874511; DOI=10.1105/tpc.17.00468;
RA Cao L., Wang L., Zheng M., Cao H., Ding L., Zhang X., Fu Y.;
RL Plant Cell 29:2306-2306(2017).
CC -!- FUNCTION: Contributes to the assembly of the acentrosomal spindle and
CC phragmoplast microtubule arrays as part of the augmin complex.
CC {ECO:0000269|PubMed:22505726}.
CC -!- SUBUNIT: Part of the augmin complex composed of 8 subunits. The complex
CC acts on microtubules and interacts with gamma-tubulin in spindles and
CC the phragmoplast. {ECO:0000269|PubMed:22505726}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SUH5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SUH5-2; Sequence=VSP_053223;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype (PubMed:22505726).
CC {ECO:0000269|PubMed:22505726}.
CC -!- SIMILARITY: Belongs to the QWRF family. {ECO:0000305}.
CC -!- CAUTION: An article reported that this protein promotes microtubule
CC reorientation in hypocotyls; however, this paper was later retracted.
CC {ECO:0000305|PubMed:23735294, ECO:0000305|PubMed:28874511}.
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DR EMBL; AL109787; CAB52444.1; -; Genomic_DNA.
DR EMBL; AL161577; CAB79789.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85799.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85800.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM68144.1; -; Genomic_DNA.
DR EMBL; BT002536; AAO00896.1; -; mRNA.
DR EMBL; AK227221; BAE99258.1; -; mRNA.
DR PIR; D85359; D85359.
DR RefSeq; NP_001329921.1; NM_001342017.1. [Q9SUH5-1]
DR RefSeq; NP_194800.1; NM_119217.3. [Q9SUH5-1]
DR RefSeq; NP_974642.1; NM_202913.1. [Q9SUH5-2]
DR AlphaFoldDB; Q9SUH5; -.
DR STRING; 3702.AT4G30710.1; -.
DR iPTMnet; Q9SUH5; -.
DR PaxDb; Q9SUH5; -.
DR PRIDE; Q9SUH5; -.
DR ProteomicsDB; 241097; -. [Q9SUH5-1]
DR EnsemblPlants; AT4G30710.1; AT4G30710.1; AT4G30710. [Q9SUH5-1]
DR EnsemblPlants; AT4G30710.2; AT4G30710.2; AT4G30710. [Q9SUH5-2]
DR EnsemblPlants; AT4G30710.3; AT4G30710.3; AT4G30710. [Q9SUH5-1]
DR GeneID; 829194; -.
DR Gramene; AT4G30710.1; AT4G30710.1; AT4G30710. [Q9SUH5-1]
DR Gramene; AT4G30710.2; AT4G30710.2; AT4G30710. [Q9SUH5-2]
DR Gramene; AT4G30710.3; AT4G30710.3; AT4G30710. [Q9SUH5-1]
DR KEGG; ath:AT4G30710; -.
DR Araport; AT4G30710; -.
DR TAIR; locus:2131949; AT4G30710.
DR eggNOG; ENOG502QYE5; Eukaryota.
DR HOGENOM; CLU_025164_2_0_1; -.
DR OrthoDB; 722743at2759; -.
DR PhylomeDB; Q9SUH5; -.
DR PRO; PR:Q9SUH5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUH5; baseline and differential.
DR Genevisible; Q9SUH5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005880; C:nuclear microtubule; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR InterPro; IPR007573; QWRF.
DR Pfam; PF04484; QWRF; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..644
FT /note="AUGMIN subunit 8"
FT /id="PRO_0000423629"
FT REGION 1..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 622..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 443..446
FT /note="QWRF motif"
FT COMPBIAS 33..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..182
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 574..575
FT /note="EE -> RP (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_053223"
SQ SEQUENCE 644 AA; 69846 MW; DC2ECE6D012DAD59 CRC64;
MDVATDTTRR RLLPSDKNNA VVATRRPRTM EVSSRYRSPT PTKNGRCPSP SVTRPTVSSS
SQSVAAKRAV SAERKRPSTP PSPTSPSTPI RDLSIDLPAS SRRLSTGRLP ESLWPSTMRS
LSVSFQSDSV SVPVSKKERP VSSSSGDRTL RPSSNIAQKH KAETTSVSRK PTPERKRSPL
KGKNNVSDLS ENSKPVDGPH SRLIEQHRWP SRIGGKITSN SLNRSLDLGD KASRGIPTSG
PGMGPSLRRM SLPLSSSSRP LHKTSSNTSS YGGLVSPTKS EDNNIARTSG AQRLLSAGSL
DRATLATAVA RLHPLPAPGS RPASPSRTSF LSSSSISRGM STSRGVSPSR GLSPSRGLSP
TRGLSPSRGL SPSRGTNTSC FARPSTPPSR GISPSRIRQT TTSTQSSTTT SVLSFITDVK
KGKKASYIED VHQLRLLHNR YLQWRFAIAR AESVMYIQRL TSEETLFNVW HAISELQDHV
TRQRIGLQQL KLEIKLNSLL NDQMVSLEDW ATLERDHVSS LVGAISDLEA NTLRLPATGG
TKADTESLKA AMSSALDVMQ AMGSSIWSLL SKVEEMNIMV TELAVVVTKE SSMQGKCEDL
LASTAIMQIE ECSLRTHLIQ TRREEGEDAE TPPPLLPLSK FPWP
