ID   AUG6_ARATH              Reviewed;         741 AA.
AC   Q94BP7; Q9FM25;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=AUGMIN subunit 6 {ECO:0000303|PubMed:22505726};
GN   Name=AUG6 {ECO:0000303|PubMed:22505726};
GN   OrderedLocusNames=At5g40740 {ECO:0000312|Araport:AT5G40740};
GN   ORFNames=MNF13.29 {ECO:0000312|EMBL:BAB08545.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|EMBL:AAK64148.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION IN THE AUGMIN COMPLEX BY MASS SPECTROMETRY, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=22505726; DOI=10.1105/tpc.112.096610;
RA   Hotta T., Kong Z., Ho C.M., Zeng C.J., Horio T., Fong S., Vuong T.,
RA   Lee Y.R., Liu B.;
RT   "Characterization of the Arabidopsis augmin complex uncovers its critical
RT   function in the assembly of the acentrosomal spindle and phragmoplast
RT   microtubule arrays.";
RL   Plant Cell 24:1494-1509(2012).
CC   -!- FUNCTION: Contributes to the assembly of the acentrosomal spindle and
CC       phragmoplast microtubule arrays as part of the augmin complex.
CC       {ECO:0000305|PubMed:22505726}.
CC   -!- SUBUNIT: Part of the augmin complex composed of 8 subunits. The complex
CC       acts on microtubules and interacts with gamma-tubulin in spindles and
CC       the phragmoplast. {ECO:0000269|PubMed:22505726}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000269|PubMed:22505726}. Cytoplasm, cytoskeleton, phragmoplast
CC       {ECO:0000269|PubMed:22505726}. Note=Preferentially localizes to
CC       microtubules minus ends. {ECO:0000269|PubMed:22505726}.
CC   -!- SIMILARITY: Belongs to the HAUS6 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08545.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB009052; BAB08545.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED94589.1; -; Genomic_DNA.
DR   EMBL; AY039971; AAK64148.1; -; mRNA.
DR   EMBL; AY079386; AAL85117.1; -; mRNA.
DR   RefSeq; NP_568585.1; NM_123439.4.
DR   AlphaFoldDB; Q94BP7; -.
DR   SMR; Q94BP7; -.
DR   STRING; 3702.AT5G40740.1; -.
DR   iPTMnet; Q94BP7; -.
DR   PaxDb; Q94BP7; -.
DR   PRIDE; Q94BP7; -.
DR   ProteomicsDB; 240916; -.
DR   EnsemblPlants; AT5G40740.1; AT5G40740.1; AT5G40740.
DR   GeneID; 834074; -.
DR   Gramene; AT5G40740.1; AT5G40740.1; AT5G40740.
DR   KEGG; ath:AT5G40740; -.
DR   Araport; AT5G40740; -.
DR   TAIR; locus:2168828; AT5G40740.
DR   eggNOG; ENOG502QUMJ; Eukaryota.
DR   HOGENOM; CLU_023157_0_0_1; -.
DR   InParanoid; Q94BP7; -.
DR   OMA; ARGHPTI; -.
DR   OrthoDB; 259004at2759; -.
DR   PhylomeDB; Q94BP7; -.
DR   PRO; PR:Q94BP7; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q94BP7; baseline and differential.
DR   Genevisible; Q94BP7; AT.
DR   GO; GO:0070652; C:HAUS complex; IEA:InterPro.
DR   GO; GO:1990498; C:mitotic spindle microtubule; IBA:GO_Central.
DR   GO; GO:0009524; C:phragmoplast; IDA:TAIR.
DR   GO; GO:0005819; C:spindle; IDA:TAIR.
DR   GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR   GO; GO:0051011; F:microtubule minus-end binding; IDA:TAIR.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0009561; P:megagametogenesis; IMP:TAIR.
DR   GO; GO:0055046; P:microgametogenesis; IMP:TAIR.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IMP:TAIR.
DR   GO; GO:0051225; P:spindle assembly; IEA:InterPro.
DR   InterPro; IPR026797; HAUS_6.
DR   InterPro; IPR028163; HAUS_6_N.
DR   PANTHER; PTHR16151; PTHR16151; 1.
DR   Pfam; PF14661; HAUS6_N; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Microtubule; Mitosis; Reference proteome.
FT   CHAIN           1..741
FT                   /note="AUGMIN subunit 6"
FT                   /id="PRO_0000434097"
FT   REGION          313..335
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          520..578
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          213..239
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        319..333
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   741 AA;  80935 MW;  EF2E940CA79D28F3 CRC64;
     MTMDREKERE LELESAMYTN CLLLGLDPNV IGLGASNGTP RVGLFRHSNP KLGEQLLYFI
     LSSLRGPAQS SKDFDKVWPI FDSAQSRDFR KVVQAIISEL ESQGALPRSN SRVSSLATCC
     GPRFVELLWQ LSLHALREVH RRTFPADVAS NPLPSSLTDV SFSHAATLLP VTKARIVLER
     RRFLKNAETA VQRQAMWSNL AHEMTAEFRG LCAEEAYLQQ ELEKLNDLRN KVKQEGEVWD
     DLVSSSSQNS HLVSKATRLW DSIMARKGQH EVLASGPIED LIAHREHRYR ISGSALLAAM
     DQSSQVPRAE LLSAHSDDSA SLADDKELSD GSYTNMHDHS LVDSFETASS QASDETLSRV
     DDRGGKINQT VDVAEVIRRW THALQRIHKQ SLLLAKANDG DGPDILRTAS DGGTSGHVES
     LAATLTEHQQ HLASFQVLIN QLKEVSPAIQ KSISECTEMV NSLPPTLPPV TRSNGQASSL
     LQSQGSGRIM EGVSNDVAEL TSTMSNVQLE KVSASPTLKL PQLFSSTPTS SGKGGNGQKR
     QTMASQVNKM ESLSEKNSTD QALSNARPDN LPTDTSSSFV HNLKKSVREA ALLIPSSAGS
     SRDSQSDEGS EHYFVPLSAT GFSRFPSETK ALPLRGSRAL TSLSEPSFLE PNVPDSFAPS
     KYSDIPDTYD DLDSFKDYDN GNGFLSVAGS NSVASDAQQS FYDIDDQVFS PPLLMDSSLL
     SDAYEDLLAP LSETEAALME H