AUG3_ARATH
ID AUG3_ARATH Reviewed; 617 AA.
AC Q0WQE7; Q9LV62;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=AUGMIN subunit 3 {ECO:0000303|PubMed:22505726};
GN Name=AUG3 {ECO:0000303|PubMed:22505726};
GN OrderedLocusNames=At5g48520 {ECO:0000312|Araport:AT5G48520};
GN ORFNames=MJE7.16 {ECO:0000312|EMBL:BAA96972.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAF00652.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP AUG1.
RX PubMed=21750235; DOI=10.1105/tpc.111.086892;
RA Ho C.M., Hotta T., Kong Z., Zeng C.J., Sun J., Lee Y.R., Liu B.;
RT "Augmin plays a critical role in organizing the spindle and phragmoplast
RT microtubule arrays in Arabidopsis.";
RL Plant Cell 23:2606-2618(2011).
RN [5]
RP IDENTIFICATION IN THE AUGMIN COMPLEX BY MASS SPECTROMETRY.
RX PubMed=22505726; DOI=10.1105/tpc.112.096610;
RA Hotta T., Kong Z., Ho C.M., Zeng C.J., Horio T., Fong S., Vuong T.,
RA Lee Y.R., Liu B.;
RT "Characterization of the Arabidopsis augmin complex uncovers its critical
RT function in the assembly of the acentrosomal spindle and phragmoplast
RT microtubule arrays.";
RL Plant Cell 24:1494-1509(2012).
CC -!- FUNCTION: Involved in microtubules reorganization during spindle and
CC phragmoplast development. Required for gamma-tubulin localization
CC during mitosis. {ECO:0000269|PubMed:21750235}.
CC -!- SUBUNIT: Part of the augmin complex composed of 8 subunits
CC (PubMed:22505726). The complex acts on microtubules and interacts with
CC gamma-tubulin in spindles and the phragmoplast (PubMed:22505726).
CC Interacts with AUG1 (PubMed:21750235). {ECO:0000269|PubMed:21750235,
CC ECO:0000269|PubMed:22505726}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:21750235}. Cytoplasm, cytoskeleton, phragmoplast
CC {ECO:0000269|PubMed:21750235}. Note=Preferentially localizes to
CC microtubules minus ends. {ECO:0000269|PubMed:21750235}.
CC -!- DISRUPTION PHENOTYPE: Lethal when homozygous.
CC {ECO:0000269|PubMed:21750235}.
CC -!- SIMILARITY: Belongs to the HAUS3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96972.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB020745; BAA96972.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95681.1; -; Genomic_DNA.
DR EMBL; AK228752; BAF00652.1; -; mRNA.
DR RefSeq; NP_199663.2; NM_124228.4.
DR AlphaFoldDB; Q0WQE7; -.
DR SMR; Q0WQE7; -.
DR STRING; 3702.AT5G48520.1; -.
DR iPTMnet; Q0WQE7; -.
DR PaxDb; Q0WQE7; -.
DR PRIDE; Q0WQE7; -.
DR ProteomicsDB; 240928; -.
DR EnsemblPlants; AT5G48520.1; AT5G48520.1; AT5G48520.
DR GeneID; 834908; -.
DR Gramene; AT5G48520.1; AT5G48520.1; AT5G48520.
DR KEGG; ath:AT5G48520; -.
DR Araport; AT5G48520; -.
DR TAIR; locus:2166071; AT5G48520.
DR eggNOG; ENOG502R4I5; Eukaryota.
DR HOGENOM; CLU_036555_0_0_1; -.
DR InParanoid; Q0WQE7; -.
DR PhylomeDB; Q0WQE7; -.
DR PRO; PR:Q0WQE7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0WQE7; baseline and differential.
DR Genevisible; Q0WQE7; AT.
DR GO; GO:0070652; C:HAUS complex; IEA:InterPro.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009524; C:phragmoplast; IDA:TAIR.
DR GO; GO:0005819; C:spindle; IDA:TAIR.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0080175; P:phragmoplast microtubule organization; IMP:TAIR.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR InterPro; IPR026206; HAUS3.
DR InterPro; IPR032733; HAUS3_N.
DR PANTHER; PTHR19378; PTHR19378; 1.
DR Pfam; PF14932; HAUS-augmin3; 1.
DR PRINTS; PR02089; HAUSAUGMINL3.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome.
FT CHAIN 1..617
FT /note="AUGMIN subunit 3"
FT /id="PRO_0000434093"
FT COILED 107..140
FT /evidence="ECO:0000255"
FT COILED 314..334
FT /evidence="ECO:0000255"
FT COILED 481..504
FT /evidence="ECO:0000255"
SQ SEQUENCE 617 AA; 69725 MW; 7ED2EECDF6D57C3F CRC64;
MSSARLCSLV AELGYEGAGK LDPDSFEWPF QYDDARPILD WICSSLRPSN VLSLAELSLY
EQFQRDGKLL EGDDLDQAYD SISAFSSRRN NQEAVFGAEE SIKEVRDATL AHKAEALELQ
RQLRRLQTQY DLLTGQSSAL IQGRRARVAA TSAVSGQITA IEDSLSARNL QMNGVLGRLA
STSQELAHYH SGEEDGIYLA YSDFHAYLAG DSACTKELNQ WFAKQLDTGP YRLVAEEGKS
KCSWVSLDDT SNMLRDLEKS QHQRVAELQR LRSIFGTSER QWIEAQVENA KQQAILLTLK
SQVTSVEAHI HFDLHSLRRK HADLVEEIST LYQKEEKLLS ETIPELCWEL AQLQDTYILQ
GDYDLKVMRQ ELYISKQKVF INHLVNQLAR HQFLKLACQL EKKNMLGAFS LLKVIESELQ
GYLSATRSRV GRCSALIQAA SDVQEQGAVD DRDSFLHGVR DLLSIHSNTQ AGLSTYVSAP
AIIQQIVALQ SDLSSLQSDL ENSLPDDRNR CINELCTHIQ NLQQLLFASS TTAQPILTPW
PLMKELDEMG KINSKLSTAV EEVTLEHRNK REIVKHHAKD VELQRRVFVD FFCNPERLRN
QVRELNALVR ARQASSS
