ID   AUF1_ARATH              Reviewed;         334 AA.
AC   Q9C9S2;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=F-box protein AUF1 {ECO:0000305};
DE   AltName: Full=F-box protein At1g78100 {ECO:0000305};
DE   AltName: Full=Protein AUXIN UP-REGULATED F-BOX PROTEIN 1 {ECO:0000303|PubMed:21653785};
GN   Name=AUF1 {ECO:0000303|PubMed:21653785};
GN   OrderedLocusNames=At1g78100 {ECO:0000312|Araport:AT1G78100};
GN   ORFNames=T11I11.4 {ECO:0000312|EMBL:AAG52096.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK13.
RX   PubMed=12169662; DOI=10.1073/pnas.162339999;
RA   Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT   "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT   superfamily of genes in Arabidopsis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
RN   [5]
RP   FUNCTION, AND INDUCTION BY AUXIN.
RX   PubMed=21653785; DOI=10.1104/pp.111.179812;
RA   Zheng X., Miller N.D., Lewis D.R., Christians M.J., Lee K.H., Muday G.K.,
RA   Spalding E.P., Vierstra R.D.;
RT   "AUXIN UP-REGULATED F-BOX PROTEIN1 regulates the cross talk between auxin
RT   transport and cytokinin signaling during plant root growth.";
RL   Plant Physiol. 156:1878-1893(2011).
CC   -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC       complexes, which may mediate the ubiquitination and subsequent
CC       proteasomal degradation of target proteins (By similarity). Involved in
CC       the control of basipetal and acropetal auxin transport by promoting the
CC       distribution and expression of the auxin transporter PIN2
CC       (PubMed:21653785). Promotes cytokinin-mediated cell expansion in the
CC       root elongation and differentiation zone, without affecting root cell
CC       division (PubMed:21653785). {ECO:0000250|UniProtKB:Q9SRV0,
CC       ECO:0000269|PubMed:21653785}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC   -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC       similarity). Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK11 and ASK13
CC       (PubMed:12169662). {ECO:0000250|UniProtKB:Q8LEA8,
CC       ECO:0000269|PubMed:12169662}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8LEA8}.
CC   -!- INDUCTION: Induced by auxin. {ECO:0000269|PubMed:21653785}.
CC   -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC       {ECO:0000250|UniProtKB:Q8LEA8}.
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DR   EMBL; AC012680; AAG52096.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE36067.1; -; Genomic_DNA.
DR   EMBL; AY054568; AAK96759.1; -; mRNA.
DR   EMBL; AY081694; AAM10256.1; -; mRNA.
DR   PIR; B96810; B96810.
DR   RefSeq; NP_565169.1; NM_106460.3.
DR   AlphaFoldDB; Q9C9S2; -.
DR   BioGRID; 29365; 10.
DR   IntAct; Q9C9S2; 7.
DR   STRING; 3702.AT1G78100.1; -.
DR   PaxDb; Q9C9S2; -.
DR   PRIDE; Q9C9S2; -.
DR   EnsemblPlants; AT1G78100.1; AT1G78100.1; AT1G78100.
DR   GeneID; 844146; -.
DR   Gramene; AT1G78100.1; AT1G78100.1; AT1G78100.
DR   KEGG; ath:AT1G78100; -.
DR   Araport; AT1G78100; -.
DR   TAIR; locus:2194719; AT1G78100.
DR   eggNOG; ENOG502QV7H; Eukaryota.
DR   HOGENOM; CLU_049279_2_0_1; -.
DR   InParanoid; Q9C9S2; -.
DR   OMA; MVEVVNE; -.
DR   OrthoDB; 988686at2759; -.
DR   PhylomeDB; Q9C9S2; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q9C9S2; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C9S2; baseline and differential.
DR   Genevisible; Q9C9S2; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0010541; P:acropetal auxin transport; IMP:TAIR.
DR   GO; GO:0010540; P:basipetal auxin transport; IMP:TAIR.
DR   GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR044809; AUF1-like.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   PANTHER; PTHR31215; PTHR31215; 1.
DR   SUPFAM; SSF81383; SSF81383; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..334
FT                   /note="F-box protein AUF1"
FT                   /id="PRO_0000283364"
FT   DOMAIN          1..49
FT                   /note="F-box"
SQ   SEQUENCE   334 AA;  36553 MW;  B1ACCC6ECA268954 CRC64;
     MDAFDAIPDP VVIDILNRVG DVKTLIRCRS VSKRFNSLAT QSESLLLQLD QILGATESDS
     EIDSPIASFF RSLFKSIHGL LPPIFSKPAN SDEILTRSPK TPAQILSGFE RIRNLEVELY
     GGDVKLEKGA AVKWKAEFGK TLKSCVIVAF RSATVNTSAA TEAAAVVDGV VESDSEFVCG
     LKTRVVWTIS ALMAASTRHY LMRDLVKDHK EMEKLIVRDS DGEGTVVMDA AGMKEYRETE
     VRGDNKESER VGERTVVPSV RMSMRHAPSL MLKSGICLEA ATLVVVRPTG VASDDNDVEL
     VTEAFAGDGD DCMYGEAVTA LLKRRRNVLE MNSF