RPOE_STRCO
ID RPOE_STRCO Reviewed; 177 AA.
AC P38133;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=RNA polymerase sigma-E factor;
GN Name=sigE; OrderedLocusNames=SCO3356; ORFNames=SCE94.07;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-28.
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=8052622; DOI=10.1073/pnas.91.16.7573;
RA Lonetto M.A., Brown K.L., Rudd K.E., Buttner M.J.;
RT "Analysis of the Streptomyces coelicolor sigE gene reveals the existence of
RT a subfamily of eubacterial RNA polymerase sigma factors involved in the
RT regulation of extracytoplasmic functions.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:7573-7577(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A3(2) / NRRL B-16638;
RX PubMed=10411727; DOI=10.1046/j.1365-2958.1999.01452.x;
RA Paget M.S.B., Leibowitz E., Buttner M.J.;
RT "A putative two-component signal transduction system regulates sigE, a
RT sigma factor required for normal cell wall integrity in Streptomyces
RT coelicolor A3(2).";
RL Mol. Microbiol. 33:97-107(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [4]
RP INVOLVEMENT IN MAINTENANCE OF CELL WALL INTEGRITY.
RC STRAIN=A3(2) / M600;
RX PubMed=17015661; DOI=10.1128/jb.00818-06;
RA Hutchings M.I., Hong H.J., Leibovitz E., Sutcliffe I.C., Buttner M.J.;
RT "The sigma(E) cell envelope stress response of Streptomyces coelicolor is
RT influenced by a novel lipoprotein, CseA.";
RL J. Bacteriol. 188:7222-7229(2006).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is required for normal cell wall integrity;
CC it is recruited by RNA polymerase to transcribe genes with cell wall-
CC related functions. It is also involved in the transcription of the dagA
CC gene coding for an extracellular agar-degrading enzyme.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: By CseB, in response to cell envelope stress.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. ECF subfamily.
CC {ECO:0000305}.
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DR EMBL; L29636; AAA26814.1; -; Genomic_DNA.
DR EMBL; AJ131213; CAA10323.1; -; Genomic_DNA.
DR EMBL; AL939116; CAB40856.1; -; Genomic_DNA.
DR PIR; T36367; T36367.
DR RefSeq; NP_627564.1; NC_003888.3.
DR RefSeq; WP_003975479.1; NZ_VNID01000023.1.
DR AlphaFoldDB; P38133; -.
DR SMR; P38133; -.
DR STRING; 100226.SCO3356; -.
DR GeneID; 1098793; -.
DR KEGG; sco:SCO3356; -.
DR PATRIC; fig|100226.15.peg.3418; -.
DR eggNOG; COG1595; Bacteria.
DR HOGENOM; CLU_047691_15_4_11; -.
DR InParanoid; P38133; -.
DR OMA; HWEQMST; -.
DR PhylomeDB; P38133; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014325; RNA_pol_sigma-E_actinobac.
DR InterPro; IPR000838; RNA_pol_sigma70_ECF_CS.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR013249; RNA_pol_sigma70_r4_t2.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF08281; Sigma70_r4_2; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02983; SigE-fam_strep; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR PROSITE; PS01063; SIGMA70_ECF; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; DNA-binding; Reference proteome;
KW Sigma factor; Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8052622"
FT CHAIN 2..177
FT /note="RNA polymerase sigma-E factor"
FT /id="PRO_0000094006"
FT DNA_BIND 128..147
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 34..47
FT /note="Polymerase core binding"
SQ SEQUENCE 177 AA; 20493 MW; 937838C601A2F601 CRC64;
MGEVLEFEEY VRTRQDALLR SARRLVPDPV DAQDLLQTAL ARTYGRWETI EDKRLADAYL
RRVMINTRTE WWRARKLEEV PTEQLPESPM DDATEQHADR ALLMDVLKVL APKQRSVVVL
RHWEQMSTEE TAAALGMSAG TVKSTLHRAL ARLREELVAR DLDARALERE ERERCAA
