ID   RPO2N_METTW             Reviewed;         515 AA.
AC   P09844;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=DNA-directed RNA polymerase subunit Rpo2N {ECO:0000305};
DE            EC=2.7.7.6 {ECO:0000250|UniProtKB:P11513};
DE   AltName: Full=DNA-directed RNA polymerase subunit B'' {ECO:0000303|PubMed:2834336};
GN   Name=rpo2N {ECO:0000305}; Synonyms=rpoB2, rpoV;
OS   Methanothermobacter thermautotrophicus (strain Winter) (Methanobacterium
OS   thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=79930;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2843811; DOI=10.1093/nar/16.16.8113;
RA   Berghoefer B., Kroeckel L., Koertner C., Truss M., Schallenberg J.,
RA   Klein A.;
RT   "Relatedness of archaebacterial RNA polymerase core subunits to their
RT   eubacterial and eukaryotic equivalents.";
RL   Nucleic Acids Res. 16:8113-8128(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 238-515.
RX   PubMed=2834336; DOI=10.1128/jb.170.5.2247-2253.1988;
RA   Schallenberg J., Moes M., Truss M., Reiser W., Thomm M., Stetter K.O.,
RA   Klein A.;
RT   "Cloning and physical mapping of RNA polymerase genes from Methanobacterium
RT   thermoautotrophicum and comparison of homologies and gene orders with those
RT   of RNA polymerase genes from other methanogenic archaebacteria.";
RL   J. Bacteriol. 170:2247-2253(1988).
CC   -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC       transcription of DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this
CC       organism) is implicated in DNA promoter recognition and in nucleotide
CC       binding. {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P11513};
CC   -!- SUBUNIT: Part of the RNA polymerase complex.
CC       {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; X08038; CAA30836.1; -; Genomic_DNA.
DR   EMBL; M20391; AAA72652.1; -; Genomic_DNA.
DR   PIR; S02194; S02194.
DR   AlphaFoldDB; P09844; -.
DR   SMR; P09844; -.
DR   PRIDE; P09844; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Transcription; Transferase.
FT   CHAIN           1..515
FT                   /note="DNA-directed RNA polymerase subunit Rpo2N"
FT                   /id="PRO_0000074033"
SQ   SEQUENCE   515 AA;  58631 MW;  7263AD7CAD456677 CRC64;
     MKKSAWGLVD AFFDKYDLVD HHIHSYNDFV SNRIQEIIDT SEPIELEQGQ YTVETGKVTI
     EKPFIKEADG SKSKIYPTEA RLRNLTYSAH MSLEMRLLKE GGSETEFEKV HIGELPVMLK
     SEICHLHGLG RDELIEKGED PADLGGYFIV NASERSIVTM EEIAPNKIIL ERIGEEDEKR
     ARAIVTSIRS GFRARISLEY RKPRKTGVFL RISFPYVPGE LPLVILLRAL GLATDQEIIT
     SISDDFNYQM IAADDIQVSL DKLKLDSDKM EEEMDEEERR EYLIRSAIKY IGNRVAKGMT
     EDYRIKRAED VIDRYLLPHI GTEPDKRLEK AVYLAEMTEM LLQVISGERK PHDKDHYTNK
     RLRVSGDLME DLFRVAFTSL TRDMSYRLER SLARGKEPSV KQAVRSDVLS ENLKHAIATG
     NWVGGRAGVS QLLDRTSYMG TLSHMRRVVS PLSRSQPHFE ARDLHPTQFG KICPNETPES
     PNCGLVKNLA LMAKISEGSD PDEIEEVIKK MGVIN