RPNC_ECOLI
ID RPNC_ECOLI Reviewed; 300 AA.
AC P31665; P75660; Q8KJQ2;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Recombination-promoting nuclease RpnC {ECO:0000303|PubMed:28096446};
DE EC=3.1.21.-;
GN Name=rpnC {ECO:0000303|PubMed:28096446}; Synonyms=yadD;
GN OrderedLocusNames=b0132, JW5010;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Merkel W.K., Nichols B.P.;
RT "Nucleotide sequence of the Escherichia coli panBCD gene cluster.";
RL Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8202364; DOI=10.1093/nar/22.9.1637;
RA Fujita N., Mori H., Yura T., Ishihama A.;
RT "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-
RT 4.1 min (110,917-193,643 bp) region.";
RL Nucleic Acids Res. 22:1637-1639(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12;
RX PubMed=28096446; DOI=10.1128/jb.00787-16;
RA Kingston A.W., Ponkratz C., Raleigh E.A.;
RT "The Rpn (YhgA-like) proteins of Escherichia coli K-12 and their
RT contribution to RecA-independent horizontal transfer.";
RL J. Bacteriol. 0:0-0(2017).
CC -!- FUNCTION: A low activity DNA endonuclease yielding 3'-hydroxyl ends
CC (Probable). Upon expression enhances RecA-independent DNA recombination
CC 2.9-fold, concomitantly reducing viability by 59% and inducing DNA
CC damage as measured by induction of the SOS repair response.
CC {ECO:0000269|PubMed:28096446}.
CC -!- DISRUPTION PHENOTYPE: A quadruple rpnA-rpnB-rpnC-rpnD deletion shows no
CC change in basal RecA-independent recombination.
CC {ECO:0000269|PubMed:28096446}.
CC -!- SIMILARITY: Belongs to the Rpn/YhgA-like nuclease family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24273.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L17086; AAA24273.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAC73243.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96710.2; -; Genomic_DNA.
DR PIR; D64736; D64736.
DR RefSeq; NP_414674.1; NC_000913.3.
DR RefSeq; WP_000339954.1; NZ_STEB01000010.1.
DR AlphaFoldDB; P31665; -.
DR SMR; P31665; -.
DR BioGRID; 4260707; 246.
DR STRING; 511145.b0132; -.
DR PaxDb; P31665; -.
DR PRIDE; P31665; -.
DR EnsemblBacteria; AAC73243; AAC73243; b0132.
DR EnsemblBacteria; BAB96710; BAB96710; BAB96710.
DR GeneID; 944781; -.
DR KEGG; ecj:JW5010; -.
DR KEGG; eco:b0132; -.
DR PATRIC; fig|1411691.4.peg.2149; -.
DR EchoBASE; EB1698; -.
DR eggNOG; COG5464; Bacteria.
DR HOGENOM; CLU_059548_1_0_6; -.
DR InParanoid; P31665; -.
DR OMA; RVYNNPF; -.
DR PhylomeDB; P31665; -.
DR BioCyc; EcoCyc:EG11748-MON; -.
DR PRO; PR:P31665; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1990238; F:double-stranded DNA endodeoxyribonuclease activity; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IDA:UniProtKB.
DR InterPro; IPR010106; RpnA.
DR InterPro; IPR006842; Transposase_31.
DR Pfam; PF04754; Transposase_31; 1.
DR TIGRFAMs; TIGR01784; T_den_put_tspse; 1.
PE 3: Inferred from homology;
KW DNA recombination; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW Reference proteome.
FT CHAIN 1..300
FT /note="Recombination-promoting nuclease RpnC"
FT /id="PRO_0000168526"
SQ SEQUENCE 300 AA; 34607 MW; F551985238862C65 CRC64;
MDAPSTTPHD AVFKQFLMHA ETARDFLEIH LPVELRELCD LNTLHLESGS FIEESLKGHS
TDVLYSVQMQ GNPGYLHVVI EHQSKPDKKM AFRMMRYSIA AMHRHLEADH DKLPLVVPIL
FYQGEATPYP LSMCWFDMFY SPELARRVYN SPFPLVDITI TPDDEIMQHR RIAILELLQK
HIRQRDLMLL LEQLVTLIDE GYTSGSQLVA MQNYMLQRGH TEQADLFYGV LRDRETGGES
MMTLAQWFEE KGIEKGIQQG RQEVSQEFAQ RLLSKGMSRE DVAEMANLPL AEIDKVINLI
