RIP_LUFAC
ID RIP_LUFAC Reviewed; 241 AA.
AC P84530;
DT 24-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 2.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=Ribosome-inactivating protein luffaculin 1 {ECO:0000303|PubMed:17470286};
DE EC=3.2.2.22;
DE AltName: Full=rRNA N-glycosidase {ECO:0000303|PubMed:17470286};
OS Luffa acutangula (Ridged gourd) (Cucumis acutangulus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX NCBI_TaxID=56866;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE OF 1-10, X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS), AND
RP GLYCOSYLATION AT ASN-77 AND ASN-84.
RC TISSUE=Seed {ECO:0000269|PubMed:17470286};
RX PubMed=17470286; DOI=10.1186/1472-6807-7-29;
RA Hou X., Chen M., Chen L., Meehan E.J., Xie J., Huang M.;
RT "X-ray sequence and crystal structure of luffaculin 1, a novel type 1
RT ribosome-inactivating protein.";
RL BMC Struct. Biol. 7:29-29(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC adenosine on the 28S rRNA.; EC=3.2.2.22; Evidence={ECO:0000305};
CC -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC RIP subfamily. {ECO:0000255}.
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DR PDB; 2OQA; X-ray; 1.00 A; A/B=1-241.
DR PDBsum; 2OQA; -.
DR AlphaFoldDB; P84530; -.
DR SMR; P84530; -.
DR iPTMnet; P84530; -.
DR EvolutionaryTrace; P84530; -.
DR GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.420.10; -; 1.
DR Gene3D; 4.10.470.10; -; 1.
DR InterPro; IPR036041; Ribosome-inact_prot_sf.
DR InterPro; IPR017989; Ribosome_inactivat_1/2.
DR InterPro; IPR001574; Ribosome_inactivat_prot.
DR InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR PANTHER; PTHR33453; PTHR33453; 1.
DR Pfam; PF00161; RIP; 1.
DR PRINTS; PR00396; SHIGARICIN.
DR SUPFAM; SSF56371; SSF56371; 1.
DR PROSITE; PS00275; SHIGA_RICIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Glycoprotein; Hydrolase;
KW Plant defense; Protein synthesis inhibitor; Toxin.
FT CHAIN 1..241
FT /note="Ribosome-inactivating protein luffaculin 1"
FT /id="PRO_0000221417"
FT ACT_SITE 159
FT /evidence="ECO:0000250|UniProtKB:P16094"
FT CARBOHYD 28
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 77
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17470286"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17470286"
FT CARBOHYD 205
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 11..24
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 43..46
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 47..53
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:2OQA"
FT TURN 66..68
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 71..76
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 86..91
FT /evidence="ECO:0007829|PDB:2OQA"
FT TURN 92..94
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 99..103
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 110..117
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 128..139
FT /evidence="ECO:0007829|PDB:2OQA"
FT TURN 143..145
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 146..156
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 158..162
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 164..172
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 182..201
FT /evidence="ECO:0007829|PDB:2OQA"
FT TURN 202..206
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 207..215
FT /evidence="ECO:0007829|PDB:2OQA"
FT STRAND 221..226
FT /evidence="ECO:0007829|PDB:2OQA"
FT HELIX 230..233
FT /evidence="ECO:0007829|PDB:2OQA"
SQ SEQUENCE 241 AA; 25891 MW; 233F2B6D72A95CF3 CRC64;
DVSFSLSGSS STSYSKFIGA LRKALPSNGT VYNITLLLSS ASGASRYTLM KLSNYDGKAI
TVAIDVTNVY IMGYLVNSTS YFFNESDAKL ASQYVFAGST IVTLPYSGNY EKLQTAAGKI
REKIPLGFPA LDSAITTLFH YDSTAAAAAF LVIIQTTAES SRFKYIEGQI IMRISKNGVP
SLATISLENE WSALSKQIQL AQTNNGTFKT PVVIMDAGGQ RVEIGNVGSK VVTKNIQLLL
N
