ID   RIPD1_PHYDI             Reviewed;          22 AA.
AC   P86144;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Dioicin-1;
DE            EC=3.2.2.22;
DE   AltName: Full=Ribosome-inactivating protein {ECO:0000303|PubMed:18704492};
DE   AltName: Full=rRNA N-glycosidase {ECO:0000303|PubMed:18704492};
DE   Flags: Fragment;
OS   Phytolacca dioica (Bella sombra tree) (Phytolacca arborea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Phytolaccaceae; Phytolacca.
OX   NCBI_TaxID=29725;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND MASS SPECTROMETRY.
RC   TISSUE=Leaf {ECO:0000269|PubMed:18704492};
RX   PubMed=18704492; DOI=10.1007/s00425-008-0796-z;
RA   Parente A., Conforto B., Di Maro A., Chambery A., De Luca P., Bolognesi A.,
RA   Iriti M., Faoro F.;
RT   "Type 1 ribosome-inactivating proteins from Phytolacca dioica L. leaves:
RT   differential seasonal and age expression, and cellular localization.";
RL   Planta 228:963-975(2008).
CC   -!- FUNCTION: Nicks pBR322 dsDNA. Has adenine polynucleotide glycosidase
CC       activity on herring sperm ssDNA. {ECO:0000269|PubMed:18704492}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000269|PubMed:18704492};
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC       {ECO:0000269|PubMed:18704492}. Golgi apparatus
CC       {ECO:0000269|PubMed:18704492}. Vacuole {ECO:0000269|PubMed:18704492}.
CC   -!- DEVELOPMENTAL STAGE: Detected in fully expanded leaves of 8 to 34 month
CC       old plants, levels peak in autumn. Also present in developing leaves of
CC       adult plants, detected in 10 and 17 day old leaves but not in 25, 40 or
CC       60 day old leaves. {ECO:0000269|PubMed:18704492}.
CC   -!- MASS SPECTROMETRY: Mass=30047.00; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18704492};
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; P86144; -.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Golgi apparatus; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Secreted; Toxin; Vacuole.
FT   CHAIN           1..>22
FT                   /note="Dioicin-1"
FT                   /id="PRO_0000372679"
FT   NON_TER         22
FT                   /evidence="ECO:0000303|PubMed:18704492"
SQ   SEQUENCE   22 AA;  2372 MW;  A565D20AFDC99888 CRC64;
     ANIVFDVESA TTGTYSTFLT SF