ID   RIPB_LUFAE              Reviewed;         250 AA.
AC   P22851;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Ribosome-inactivating protein luffin-B;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
OS   Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX   NCBI_TaxID=3670;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=1368666; DOI=10.1271/bbb1961.55.229;
RA   Islam M.R., Hirayama H., Funatsu G.;
RT   "Complete amino acid sequence of luffin-b, a ribosome-inactivating protein
RT   from sponge gourd (Luffa cylindrica) seeds.";
RL   Agric. Biol. Chem. 55:229-238(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000305}.
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DR   PIR; JN0108; JN0108.
DR   PDB; 1NIO; X-ray; 2.00 A; A=1-250.
DR   PDBsum; 1NIO; -.
DR   AlphaFoldDB; P22851; -.
DR   SMR; P22851; -.
DR   EvolutionaryTrace; P22851; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR017988; Ribosome_inactivat_prot_CS.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antiviral defense; Direct protein sequencing; Hydrolase;
KW   Plant defense; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..250
FT                   /note="Ribosome-inactivating protein luffin-B"
FT                   /id="PRO_0000221416"
FT   ACT_SITE        160
FT                   /evidence="ECO:0000250"
FT   STRAND          3..6
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           12..25
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          28..32
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          35..38
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           44..47
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          48..54
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          60..66
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   TURN            67..69
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          72..77
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          80..83
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           87..92
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   TURN            93..95
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          100..104
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           111..118
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           122..124
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           129..140
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   TURN            144..146
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           147..157
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           159..163
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           165..173
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          175..177
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           183..191
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           193..203
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   TURN            204..208
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          209..217
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   STRAND          223..228
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           232..235
FT                   /evidence="ECO:0007829|PDB:1NIO"
FT   HELIX           246..248
FT                   /evidence="ECO:0007829|PDB:1NIO"
SQ   SEQUENCE   250 AA;  27293 MW;  F01A8DC8A1078700 CRC64;
     ANVSFSLSGA DSKSYSKFIT ALRKALPSKE KVSNIPLLLP SASGASRYIL MQLSNYDAKA
     ITMAIDVTNV YIMGYLVNST SYFANESDAK LASQYVFKGS TLVTIPYSGN YERLQNAAGK
     IREKIPLGFR ALDSALTSIF HYDSTAAAAA FLVILQTTAE ASRFKYIEGQ IIERIPKNEV
     PSPAALSLEN EAWSLLSKQI QLAQTNNGAF RTPVVIIDNK GQRVEITNLA SKVQIKDVNS
     KLLLNKQNIA