ID   RIP9_SAPOF              Reviewed;         253 AA.
AC   Q7M1Z2;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Ribosome-inactivating protein saporin-9;
DE            Short=SAP-9;
DE            Short=SO-9;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
GN   Name=SAP9;
OS   Saponaria officinalis (Common soapwort) (Lychnis saponaria).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Caryophyllaceae; Caryophylleae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1] {ECO:0000305, ECO:0000312|PIR:A58923}
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000269|PubMed:11180645};
RX   PubMed=11180645; DOI=10.1002/jms.102;
RA   Di Maro A., Ferranti P., Mastronicola M., Polito L., Bolognesi A.,
RA   Stirpe F., Malorni A., Parente A.;
RT   "Reliable sequence determination of ribosome-inactivating proteins by
RT   combining electrospray mass spectrometry and Edman degradation.";
RL   J. Mass Spectrom. 36:38-46(2001).
CC   -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein
CC       synthesis in animal cells. {ECO:0000250|UniProtKB:P20656}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000250|UniProtKB:P20656};
CC   -!- MASS SPECTROMETRY: Mass=28495; Mass_error=2; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11180645};
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1
CC       RIP subfamily. {ECO:0000255}.
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DR   PIR; A58923; A58923.
DR   AlphaFoldDB; Q7M1Z2; -.
DR   SMR; Q7M1Z2; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.420.10; -; 1.
DR   Gene3D; 4.10.470.10; -; 1.
DR   InterPro; IPR036041; Ribosome-inact_prot_sf.
DR   InterPro; IPR017989; Ribosome_inactivat_1/2.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   PANTHER; PTHR33453; PTHR33453; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   SUPFAM; SSF56371; SSF56371; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plant defense;
KW   Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..253
FT                   /note="Ribosome-inactivating protein saporin-9"
FT                   /id="PRO_0000253946"
FT   ACT_SITE        176
FT                   /evidence="ECO:0000250|UniProtKB:P33186"
SQ   SEQUENCE   253 AA;  28494 MW;  22915D5494842389 CRC64;
     VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKDKF LRINFQSSRG
     TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAELT ALFPEATAAN HKALEYTEDY
     HSIEKNAQIT EGDKSRKELG LGINLLSSTM DTVNKKVRVV KNEARFLLIA IQMTAEAVRF
     RYIQNLVTKN FPNKFNSENK VIKFEVNWKK ISTAIHGDAK NGVFNKDYDF GFGKVRLVKD
     LQMGLLMHLG KPK