RGM1_YEAST
ID RGM1_YEAST Reviewed; 211 AA.
AC Q00453; D6W005;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Probable transcription repressor protein RGM1;
GN Name=RGM1; OrderedLocusNames=YMR182C; ORFNames=YM8010.12C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1923755; DOI=10.1093/nar/19.18.4873;
RA Estruch F.;
RT "The yeast putative transcriptional repressor RGM1 is a proline-rich zinc
RT finger protein.";
RL Nucleic Acids Res. 19:4873-4877(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- INTERACTION:
CC Q00453; P39940: RSP5; NbExp=2; IntAct=EBI-15073, EBI-16219;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- INDUCTION: Under the control of the inducible GAL10 promoter.
CC -!- DOMAIN: The Pro-rich region of rgm1 attached to a heterologous DNA
CC binding domain is able to repress the expression of the target gene.
CC -!- MISCELLANEOUS: Present with 1230 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X59861; CAA42521.1; -; Genomic_DNA.
DR EMBL; Z49808; CAA89915.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10079.1; -; Genomic_DNA.
DR PIR; S55129; S55129.
DR RefSeq; NP_013907.1; NM_001182688.1.
DR AlphaFoldDB; Q00453; -.
DR BioGRID; 35360; 59.
DR DIP; DIP-4422N; -.
DR IntAct; Q00453; 3.
DR STRING; 4932.YMR182C; -.
DR MaxQB; Q00453; -.
DR PaxDb; Q00453; -.
DR PRIDE; Q00453; -.
DR EnsemblFungi; YMR182C_mRNA; YMR182C; YMR182C.
DR GeneID; 855220; -.
DR KEGG; sce:YMR182C; -.
DR SGD; S000004794; RGM1.
DR VEuPathDB; FungiDB:YMR182C; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000176682; -.
DR HOGENOM; CLU_1305731_0_0_1; -.
DR InParanoid; Q00453; -.
DR OMA; PNATRMF; -.
DR BioCyc; YEAST:G3O-32870-MON; -.
DR PRO; PR:Q00453; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q00453; protein.
DR GO; GO:0000785; C:chromatin; IDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:SGD.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..211
FT /note="Probable transcription repressor protein RGM1"
FT /id="PRO_0000046817"
FT ZN_FING 19..44
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 50..73
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 178..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 6..11
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 178..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 114
FT /note="A -> V (in Ref. 1; CAA42521)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 211 AA; 23855 MW; FADCE4757FE7D372 CRC64;
MRGKQPKRNK DNASVKRNYR CVGYPDCNMS FNRTEHLARH IRKHTGEKPF QCNICLKFFS
RIDNLRQHQS SVHSDVDLMS LRRLQQSANS TANDPNATRM FPQLRPYGIV VQPAPVPYNL
PISTPASPQD TISLYAPPYF PHPMPSAPIP LPHQPPPLPI YSYMQPLFLN HTPIQNHNIV
ELPPDSSDTP ASPSKVQSFD QAKDASPNAK K
