ID   REX_STRCO               Reviewed;         258 AA.
AC   Q9WX14;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Redox-sensing transcriptional repressor Rex {ECO:0000255|HAMAP-Rule:MF_01131};
GN   Name=rex {ECO:0000255|HAMAP-Rule:MF_01131}; OrderedLocusNames=SCO3320;
GN   ORFNames=SCE68.18c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   CHARACTERIZATION, AND MUTAGENESIS OF GLY-102.
RC   STRAIN=A3(2) / M600;
RX   PubMed=12970197; DOI=10.1093/emboj/cdg453;
RA   Brekasis D., Paget M.S.B.;
RT   "A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor
RT   A3(2).";
RL   EMBO J. 22:4856-4865(2003).
CC   -!- FUNCTION: Modulates transcription of respiratory genes in response to
CC       changes in cellular NADH/NAD(+) redox state. May play a general role as
CC       a sensor of cellular redox balance.
CC   -!- SUBUNIT: Homodimer.
CC   -!- INTERACTION:
CC       Q9WX14; Q9WX14: rex; NbExp=2; IntAct=EBI-15532671, EBI-15532671;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the transcriptional regulatory Rex family.
CC       {ECO:0000255|HAMAP-Rule:MF_01131}.
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DR   EMBL; AL939116; CAB45354.1; -; Genomic_DNA.
DR   PIR; T36268; T36268.
DR   RefSeq; NP_627530.1; NC_003888.3.
DR   RefSeq; WP_003975516.1; NZ_VNID01000025.1.
DR   AlphaFoldDB; Q9WX14; -.
DR   SMR; Q9WX14; -.
DR   DIP; DIP-48429N; -.
DR   STRING; 100226.SCO3320; -.
DR   GeneID; 1098754; -.
DR   KEGG; sco:SCO3320; -.
DR   PATRIC; fig|100226.15.peg.3380; -.
DR   eggNOG; COG2344; Bacteria.
DR   HOGENOM; CLU_061534_0_1_11; -.
DR   InParanoid; Q9WX14; -.
DR   OMA; HEQRKAG; -.
DR   PhylomeDB; Q9WX14; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0051775; P:response to redox state; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   HAMAP; MF_01131; Rex; 1.
DR   InterPro; IPR003781; CoA-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR009718; Rex_DNA-bd_C_dom.
DR   InterPro; IPR022876; Tscrpt_rep_Rex.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR35786; PTHR35786; 1.
DR   Pfam; PF02629; CoA_binding; 1.
DR   Pfam; PF06971; Put_DNA-bind_N; 1.
DR   SMART; SM00881; CoA_binding; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA-binding; NAD; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..258
FT                   /note="Redox-sensing transcriptional repressor Rex"
FT                   /id="PRO_0000097916"
FT   DNA_BIND        26..65
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT   REGION          219..258
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         100..105
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01131"
FT   MUTAGEN         102
FT                   /note="G->A: Loss of redox sensing."
FT                   /evidence="ECO:0000269|PubMed:12970197"
SQ   SEQUENCE   258 AA;  26809 MW;  84B82E3CDB4432A6 CRC64;
     MATGRAHRPA TRSRGIPEAT VARLPLYLRA LTALSERSVP TVSSEELAAA AGVNSAKLRK
     DFSYLGSYGT RGVGYDVEYL VYQISRELGL TQDWPVVIVG IGNLGAALAN YGGFASRGFR
     VAALIDADPG MAGKPVAGIP VQHTDELEKI IQDDGVSIGV IATPAGAAQQ VCDRLVAAGV
     TSILNFAPTV LNVPEGVDVR KVDLSIELQI LAFHEQRKAG EEAAADGAAP PVAARKQQRS
     TGSADQGPDG DVPAVMPA