ID   RDRP_RDVA               Reviewed;        1444 AA.
AC   Q02119;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   29-SEP-2021, entry version 66.
DE   RecName: Full=RNA-directed RNA polymerase P1;
DE            EC=2.7.7.48;
DE   AltName: Full=Replicase;
OS   Rice dwarf virus (isolate Akita) (RDV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus.
OX   NCBI_TaxID=142803;
OH   NCBI_TaxID=114194; Alopecurus aequalis.
OH   NCBI_TaxID=90397; Echinochloa crus-galli (Barnyard grass) (Panicum crus-galli).
OH   NCBI_TaxID=94400; Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=147271; Paspalum.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=isolate Akita, and Isolate H;
RX   PubMed=1529532; DOI=10.1016/0042-6822(92)91210-l;
RA   Suzuki N., Tanimura M., Watanabe Y., Kusano T., Kitagawa Y., Suda N.,
RA   Kudo H., Uyeda I., Shikata E.;
RT   "Molecular analysis of rice dwarf phytoreovirus segment S1: interviral
RT   homology of the putative RNA-dependent RNA polymerase between plant- and
RT   animal-infecting reoviruses.";
RL   Virology 190:240-247(1992).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in both
CC       transcription and genome replication. Together with the capping enzyme
CC       P5 and protein P7, forms an enzyme complex positioned near the channels
CC       situated at each of the five-fold vertices of the core (By similarity).
CC       {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cytoplasm
CC       {ECO:0000250}. Note=Located inside the inner capsid. Found in the
CC       interior of spherical cytoplasmic structures, called virus factories,
CC       that appear early after infection and are the site of viral replication
CC       and packaging. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; D90198; BAA14222.1; -; Genomic_RNA.
DR   EMBL; D10222; BAA01074.1; -; mRNA.
DR   PIR; A43377; A43377.
DR   PRIDE; Q02119; -.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   InterPro; IPR014383; RNA-dir_poll_phytoreovirus.
DR   PIRSF; PIRSF000822; RdRPol_RDV; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Nucleotide-binding; Nucleotidyltransferase;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..1444
FT                   /note="RNA-directed RNA polymerase P1"
FT                   /id="PRO_0000222777"
FT   DOMAIN          690..897
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          157..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         9
FT                   /note="I -> V (in strain: Isolate H)"
FT   VARIANT         92
FT                   /note="K -> E (in strain: Isolate H)"
FT   VARIANT         100
FT                   /note="K -> R (in strain: Isolate H)"
FT   VARIANT         152
FT                   /note="V -> A (in strain: Isolate H)"
FT   VARIANT         225
FT                   /note="C -> V (in strain: Isolate H)"
FT   VARIANT         244
FT                   /note="L -> I (in strain: Isolate H)"
FT   VARIANT         279
FT                   /note="S -> R (in strain: Isolate H)"
FT   VARIANT         323
FT                   /note="N -> S (in strain: Isolate H)"
FT   VARIANT         426
FT                   /note="N -> S (in strain: Isolate H)"
FT   VARIANT         445
FT                   /note="M -> T (in strain: Isolate H)"
FT   VARIANT         502
FT                   /note="I -> T (in strain: Isolate H)"
FT   VARIANT         726
FT                   /note="S -> T (in strain: Isolate H)"
FT   VARIANT         746
FT                   /note="K -> R (in strain: Isolate H)"
FT   VARIANT         773
FT                   /note="K -> R (in strain: Isolate H)"
FT   VARIANT         1332
FT                   /note="K -> R (in strain: Isolate H)"
FT   VARIANT         1369
FT                   /note="L -> F (in strain: Isolate H)"
SQ   SEQUENCE   1444 AA;  164144 MW;  900F7EB7C3EBEAF7 CRC64;
     MDLARSDIIP HLLCLFQEII QANIQKVSEA YDLELKIMNI LTLWRNGSDN LISDNDDYMK
     KGLFFSRSND PLALQARYAQ MYDDLFKLNN YKVPDDVVRK HDTKILDIIL KESSVPFWYD
     ISDDEAHESM LPEFRLQDIH EFRLNLKRVA VVPDESEEIQ MDESQSDKRR RKKRMEKSRP
     VWLSGSENDR RIELNDSLKP SQKFETKLSS YLLNRLMNEM NPHYCGHPLP ALFVTLIMLK
     AYSLKNKFFS YGIRYMELVC NEIGGPDLNT RTFPVLFGSD GSFVGTRVYS HYPIKLRMIL
     NDLTYLLTYS DLHKFQEFEL DVNDEVLLHM LHSPNDGRQL KKAVTRLNLY YGLKFNPKTT
     DCGVVNGMDF THKHPITKTA DFTSPVLPMT NSFNKAEICY GHSSKILNRA VFTDTVRGYI
     REDLKNVADL DLPKLHEHVS KLVDMRVNYT IIYDLMFLRV MLNLGGYSRS NQITDFRKTI
     DEITKMNEDF LSGADPEKNI DILNAWMAPT MEDCGYRLTK SILFGKFRKA KYPSDLEAKS
     NIDYYVTARS AGIGNLRISI ETDKRKYKVR TTSKSAFVNA MGSGILDVNP VSNEPMMLTD
     YLLTQTPETR ANLEAAIDSG SISDSELMRI LGQNSIGSRS TTAWRPVRPI YINVLQAHLA
     QAFIIGPHIN ATVNQHESQP TSLWFTGDDL GVGFATLYQN GTADIIAPAI EASSTGKALS
     VLADCSSWDQ TFLTATIIPY YNGIKKALLE YQQADMRNFY MIDSSRTGVP GMKLSEIVDW
     FNSFQTKRIF NASYLKERHS FVVKYMWSGR LDTFFMNSVQ NALITRRIAE EVSLKVSNTG
     LSWFQVAGDD AIMVYDGSSI STTEQVTRVN EITVRNYEES NHIINPQKTV ISHISGEYAK
     IYYYAGMHFR DPSIQLHESE KDSGASDVTE SLRGFGQVIY EYNKRAIGTL RVNALYGRLI
     AGLAYSVNVR RYDASKRTYA NMKYYPPPTS VIAPAAFKGG LGLSFTGLSL NEVLFIKMHL
     HEAVSQGLHV ISMISFEANE VVSNSLSAYY LKDQKDLLRD MKLGKHLEKV KGISFKSSDL
     AFSGSDFSQG LNLKRESIDK VKLEVSRKSI RDLRSSGISV PSTHAYENLP YASLHQSFKS
     LKVDRDTSKF TNERLLVSLL EYKSDIPRVS VTSQYPVYDL INISKVDELN VRSGGPVRFI
     STPIEGKLLE ENIGTRQGVQ FKNRGYGGSQ EVLHFIRSNG LVITEQALID LIIKSGVLLM
     INPQRGLIDL FQSLSGDTAS SMHLANFFMA EKPHWEDNAI SLTIAGSLLE NCDSRIENVK
     NFVSVLATGM QKDLQRMFYY VGFVYYAQRL IWSGGHSSKI FVSIDEDKLA DFLRGSKPIT
     RRRKAMAGTK REPINLSANF SYEISEPDRE ISEYDPLVLC HPLSMPFFGN WQEKYSVMQS
     DEQM