ID   RDRP_PAV                Reviewed;         973 AA.
AC   Q9J7Z2;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   29-SEP-2021, entry version 69.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RdRp;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA replicase;
DE            Short=Protein A;
OS   Pariacoto virus (PaV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC   Nodamuvirales; Nodaviridae; Alphanodavirus.
OX   NCBI_TaxID=103782;
OH   NCBI_TaxID=37547; Spodoptera eridania (Southern armyworm).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10799587; DOI=10.1128/jvi.74.11.5123-5132.2000;
RA   Johnson K.N., Zeddam J.-L., Ball L.A.;
RT   "Characterization and construction of functional cDNA clones of Pariacoto
RT   virus, the first Alphanodavirus isolated outside Australasia.";
RL   J. Virol. 74:5123-5132(2000).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AF171942; AAF71691.1; -; Genomic_RNA.
DR   RefSeq; NP_620109.1; NC_003691.1.
DR   GeneID; 956347; -.
DR   KEGG; vg:956347; -.
DR   Proteomes; UP000204174; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043647; Noda_Vmethyltr_dom.
DR   InterPro; IPR001205; RNA-dir_pol_C.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   Pfam; PF19222; Noda_Vmethyltr; 1.
DR   Pfam; PF00680; RdRP_1; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..973
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000222449"
FT   DOMAIN          558..683
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          853..973
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        880..894
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        952..973
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   973 AA;  108099 MW;  C08835451D46EA1E CRC64;
     MEEHIPLPSQ YESPKALPPR VPSSRWLRSL RPRLANSCLA LKIRAHESLV KIRLCKPYDA
     QSRSKIIEKV IERRETRKTL AHQLKDLKLV PVARDHTHGR AAKFRTSANI WMNEAMRAAG
     YEPYNVSMSN HDIERGNRYF YFAKDLTIPY RNDPVSDNTG FVFCDVDYYA DMEKWMQHFK
     PMLLYTLVPE SLSYHCDDHS FHVNDDRVFF DVRGGASYSH QLWDYTGDTI CVRGKNKELL
     VFTIEQKCIQ GDPHRRIIFL EPAARVAWPF YKPMKVEVGL KRKCMTAGQV NVLYEPIDDK
     ISLSASGSRH TVETTGRTLA AITARMKNKT SPPMVADVER ILRDAGDKEA CVNAPILFEL
     IPEAKFRVNV VKTTATPTHF QPLGPLRTED GETCGHAVTT TLATAPALLP MRGVNSDVAT
     VNGRVKKPAN TVIPFKEYKE YASEFVEFLV PEPGVGHPWD TAAVREVQDN RQQKARINMV
     AATVSTHSSN RLKAFIKAEA YAATNDPRNI TTMAPELTLM MSCFTYAFKE KILYEQPWYG
     PGKTPKQVGR RLQSIAKHGT LESDYSRFDG SISEWLQKNV VKAAYMRFFK EHQRTEFQSW
     FSKVFMQMGT TTAGVRYEAG WGTRSGSPIT TDGNTMLNAF VVYCCYRKLC HTPAEAWRKL
     SQGALLTGDD AVLAHENGLE PALLDVVKNL GLKVEAKVNG PDDPVSFCGR IYPRLSDCIT
     SFQDPLRTIP KLHLTTNKGV SPEQAAANRA HGYLATDKAT PIIGTWARRV IELTGDLKVK
     GATREEQYKL SNAHQQLDPS LIETAMANIL GIDVGELKAL DKAVSEAKAL DQMPVVLGNC
     YKHKIEAVVG GEVVGPGPRV ETVEPNHEQS SGTPEVVPEM AGHSERRDKS SNPRPGGKAE
     GLSSKAGKPR VPTRPAADRK AAAGSGNRRG PTNGRRPIRD RAPRGGGRPN PGTTPPVSNS
     ETTTTTAVVH ASA