ID   RDRP_OLV2I              Reviewed;         787 AA.
AC   Q83944;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 75.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Olive latent virus 2 (isolate Italy) (OLV-2).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Oleavirus.
OX   NCBI_TaxID=650489;
OH   NCBI_TaxID=4145; Olea.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8887501; DOI=10.1099/0022-1317-77-10-2637;
RA   Grieco F., Dell'Orco M., Martelli G.P.;
RT   "The nucleotide sequence of RNA1 and RNA2 of olive latent virus 2 and its
RT   relationships in the family Bromoviridae.";
RL   J. Gen. Virol. 77:2637-2644(1996).
RN   [2]
RP   SEQUENCE REVISION.
RA   Grieco F.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; X94347; CAA64073.1; -; Genomic_RNA.
DR   RefSeq; NP_620043.1; NC_003674.1.
DR   GeneID; 991142; -.
DR   KEGG; vg:991142; -.
DR   Proteomes; UP000000412; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..787
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000402416"
FT   DOMAIN          505..619
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   787 AA;  90690 MW;  34A49559148BA617 CRC64;
     MAGVLYDEEY VPQEPDYLYC PYEWEGEDPT QCDANVPSVS YLVEAVKKSF TEGNHDMLIS
     CESYHAECCE GPLMNKGLTL STYFMSYPGI SLPAFGGGVI HVTSARVCMQ AFYAVTPGRC
     SCSCGTQFIY RAKGGTGLTF DATNTISSAF KFEDGKVKWL TEDLGLALLA GCFLKYVPIP
     HEIEIKIERK VEPVSALDFW EPEGLISDVH FVHPQERALA PEPPMDVEMV RLVRTELFEH
     FNPTLLLNAR SDFSGEKLKE VELPRAQSEP LVPRVCLPRG DDALIDQFFI DKAPEHFNRE
     PATDQWQVES DDFTVDFPTQ VRLRERDEDW FRRKPDFAVS SIRTGLEMPR IATKKEMLMA
     FSKRNVGAPQ MVEVPQEIHV KRLADRFFRT FLKEGGWDKG DGHYWDDFFH RKGRKVFSLE
     NCYDDPLSSY LVMLKSAGKF SLDLNFPVRS VPQTITYHEK WVTEVFSPMF LQVMARFFSR
     LQDWVVVPAG PMADFSRVWP SFEGKCLTEI DLSKFDKSQG KLLHDVQREI FLRLGFPPIW
     CDWWFKFHES SYLNDKNLGI AFSVDYQRRT GNTATYFGNT LVTMIMMAEV YELDSLPFCG
     LFSGDDNLIV TDRPLEGRVS LFPNMFNMEA KVMRPGQNYM CSKYLCTVDG RVTPVPDPFK
     LLKKAGASCP LEHLDDFYES FLDYTKYLVR DEVRTLIPRL MAHRYQVSMD EAGVALDQIM
     SWRKSKTQFF KHFQYRKPIK DSLEAVKSDF LSAFGKVDDR IRVWRGRRTN HKKEVDALKV
     PLVSFRE