ID   RDRP_NMV                Reviewed;        1643 AA.
AC   P15095;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=186 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Narcissus mosaic virus (NMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12180;
OH   NCBI_TaxID=39639; Narcissus pseudonarcissus (Daffodil).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2732689; DOI=10.1099/0022-1317-70-2-267;
RA   Zuidema D., Linthorst H.J.M., Huisman M.J., Asjes C.J., Bol J.F.;
RT   "Nucleotide sequence of narcissus mosaic virus RNA.";
RL   J. Gen. Virol. 70:267-276(1989).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexvirus/carlavirus RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; D13747; BAA02891.1; -; Genomic_RNA.
DR   PIR; JT0470; RRWGNV.
DR   RefSeq; NP_040778.1; NC_001441.1.
DR   PRIDE; P15095; -.
DR   GeneID; 1494016; -.
DR   KEGG; vg:1494016; -.
DR   Proteomes; UP000008865; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1643
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222549"
FT   DOMAIN          59..224
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          828..996
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          997..1132
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1372..1479
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          686..711
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1131..1155
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1587..1620
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         868..875
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1643 AA;  186305 MW;  ADAE6FCF0FF2EC43 CRC64;
     MAKVRAALER IRDPSVQTAL SEAAYTHVRP VLKESLVNCP YALSNDEADC LESFGITVNP
     YANQTHTHAA CKVIENRMLE IVGMHLPKHS CTMLFLKRSK LRYMRRAAIL KDVFLNKDVE
     PKDLFRYDRD TIRSRLQDID TKIAYMSDTL HFMSRREIVQ LFEDSPKLET LLATVVLPVE
     ALHKRTSLYP SLYSINYSAK GFEYIPGNHG GGSYFHPYTT LEWLKVRMIN AEDFHKLSTG
     FILTFQLVES LGANHLFIVQ KAKLLTPQMR TFCRDSLVTL PQVFCPAAMN ANRPLSKTKA
     MQMLLYCKSV KQVTERDIYA KIRQIIPTSE LELYDPDEIV HLANYFFFVS SLDSITCYED
     LLSSDIWMRL TRPLRTAVRK FVELFKGKQD FDKLLIALKW QPFSYSLEPV DFTAYFVSRR
     VRTLAKMEDI SWHQAAELAR RLESEPDLLS WEDLCSKPIA DPLASVSTPP LATLTSPAIS
     ESRVTDTSSP KLTNISVESI LHPTSSNHAT IAESLNPKFC GWTRGDFHHM IVNQPSDRLK
     GCRSWSYTTS PDVDLMLENL LLTPIPWESR LSDILLTLNT PANACCIQIL DCSAASAWVD
     WSVQPIKTFP VAFVGMGETT LTFEDDSTLP LKEGEFVFFP PEWLARHKYQ IKTASNLHLC
     ATFLVLDTTL LGETLANNCI EPEPLRPTAS RKSCQPTPSV DKSGADSPPE KQIVTPIVDA
     AVLSCRPKLQ DVTKPTKVLM AAETSDSVAD SLPWASWVNL LQKHGFKGNQ QQIAQDGQLI
     IPISDIRKLP HIPFPEEVPE TLRETLKNIK RFPVEITMQH KRAGSYDSDI KNNRTGKLLS
     QMDNKWKAAF AYKLQQEDRK VCGTIIHGCG GSGKSFAIQE WMRSLKEDQS VVTVVTPTVL
     LRNDWQTKLP ILPADVFKTF EKSVIQPCNP ILVFDDYTKL PPGLIESVVM HHQNVVFIIL
     TGDNRQSVYH ETNPEAYIAA LPEAVEIFSP YCEFYLNATH RNVKDLANKL GVYSEREGKL
     KVNFASHHLK ASRIPMLVPS TMKRNAMFDM GHHSMTYAGC QGLTAPKIQI LLDNHTQFCS
     ERVLYTCLSR AVDRIHFINT GPTTGDYWAK LESTPYLKAF IDTYRDEKTE VYNSQPASAE
     PTEPEAPATH FPTAPKPLLE PLVEKLTDKE AREIFSPAFG HSNAIQTEDS VVQLFQHQQA
     KDETLYWATI DTRLAISTPE ANLREFNMKR DIGDILFMNY AKLMCLPPEP VPFEERLWKI
     SADEVRNTYI SKPIGNLVNA ASRQSPDFPK NKIALFLKSQ WVKKTEKLGT LKVKPGQTIA
     SFMQETVMLY GTMARYLRKM RRRFQPDNIF INCETTPEDL DKFIKSQWDF SRPAHTNDFT
     AFDQSQDGAM LQFEVIKAKF FNIPAEIIEG YIYIKLNAAI FLGTLGIMRL SGEGPTFDAN
     TECSIAYNAT RFHITDDTAQ VYAGDDMALD RVSIEKDSFN RLEKQLKLTS KPMFPKQVKG
     DYAEFCGWVM TPAGIIKHSL KMHASIQLQK KINNIKESAR SYALDLRYAY KLGDELQEHL
     NEVEADYHQQ SVRDMHLLHQ QDVLNKGSAS PPHVFEKTAD ANTAGSSKTH KRNALKKKKQ
     TRIAEILPSP DATGLSSLPF RFF