RDRP_BSMV
ID RDRP_BSMV Reviewed; 771 AA.
AC Q80873; Q07117;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 29-SEP-2021, entry version 72.
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=Gamma-A protein;
OS Barley stripe mosaic virus (BSMV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Hordeivirus.
OX NCBI_TaxID=12327;
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3590624; DOI=10.1016/0042-6822(87)90211-x;
RA Gustafson G., Hunter B., Hanau R., Armour S.L., Jackson A.O.;
RT "Nucleotide sequence and genetic organization of barley stripe mosaic virus
RT RNA gamma.";
RL Virology 158:394-406(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2586501;
RA Kozlov Y.V., Afanasiev B.N., Rupasov V.V., Golova I.B., Kulaeva O.I.,
RA Dolia V.V., Atabekov I.G., Baev A.A.;
RT "Primary structure of RNA 3 of barley stripe mosaic virus and its
RT variability.";
RL Mol. Biol. (Mosk.) 23:1080-1090(1989).
RN [3]
RP FUNCTION.
RX PubMed=2209552; DOI=10.1002/j.1460-2075.1990.tb07553.x;
RA Petty I.T., French R., Jones R.W., Jackson A.O.;
RT "Identification of barley stripe mosaic virus genes involved in viral RNA
RT replication and systemic movement.";
RL EMBO J. 9:3453-3457(1990).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=28388677; DOI=10.1371/journal.ppat.1006319;
RA Zhang K., Zhang Y., Yang M., Liu S., Li Z., Wang X., Han C., Yu J., Li D.;
RT "The Barley stripe mosaic virus gammab protein promotes chloroplast-
RT targeted replication by enhancing unwinding of RNA duplexes.";
RL PLoS Pathog. 13:e1006319-e1006319(2017).
CC -!- FUNCTION: RNA-directed RNA polymerase plays an essential role in the
CC virus replication. {ECO:0000269|PubMed:2209552}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBCELLULAR LOCATION: Host chloroplast envelope
CC {ECO:0000269|PubMed:28388677}. Note=The viral replication sites are
CC located at the host chloroplast membrane.
CC {ECO:0000269|PubMed:28388677}.
CC -!- MISCELLANEOUS: The genome of this virus consists of three linear,
CC positive, single-stranded RNAs encapsidated in separate virions
CC designated RNA-alpha, RNA-beta and RNA-gamma. Three proteins (alpha-A,
CC beta-A and gamma-A) are translated directly from these genomic RNAs and
CC the remaining proteins encoded on RNA-beta (beta-B, beta-C and beta-D)
CC and RNA-gamma (gamma-B) are expressed via three subgenomic messenger
CC RNAs. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC RNA polymerase family. {ECO:0000305}.
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DR EMBL; M16576; AAA66600.1; -; Genomic_RNA.
DR EMBL; X52774; CAA36983.1; -; Genomic_RNA.
DR PIR; PN0105; PN0105.
DR PIR; PN0108; PN0108.
DR RefSeq; NP_604481.1; NC_003478.1.
DR GeneID; 962675; -.
DR KEGG; vg:962675; -.
DR Proteomes; UP000001667; Genome.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF00978; RdRP_2; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT CHAIN 1..771
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000222493"
FT DOMAIN 524..637
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT CONFLICT 118..119
FT /note="KQ -> I (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="P -> L (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="E -> K (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="Q -> R (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 362
FT /note="E -> D (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 488
FT /note="E -> V (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 725
FT /note="D -> N (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
FT CONFLICT 744
FT /note="I -> M (in Ref. 2; CAA36983)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 771 AA; 87304 MW; DA15B7CDBEEF9053 CRC64;
MDVVKKFAVM SVTVVAGPVL TLSSPVVVTF GTSLIAVSLV KRLLQEQPRV IAHDHEHYPG
GSESSSSSCA TAPILRNLSR DQCDSENIGC SSSACSPSEI VKVTRQVVEV ERGLYRDKQQ
FGMDVVKKFA VMSVTVVAGP VLTLSSPVVV TFGTSLIAVS LVKRLLQEQP RVIAHDHEHY
PGGSESSSSS CATAPILRNL SRDQCDSENI GCSSSACSPS EIVKVTRQVV EVERGLYRDI
FQDNEIPSVM EEKLQKLLYS EGEKIRRRCQ FEASTMHSRK VKVPEVGTIP DIQTWFDATF
PGNSVRFSDF DGYTVATEDI NMDVQDCRLK FGKTFRPYEF KESLKPVLRT AMPEKRQGSL
IESVLAFRKR NLAAPRLQGA LNEWHTIENV LKKALKVFFF EDLIDRTDHC TYESALRWWD
KQSVTARAQL VADQRRLCDV DFTTYNFMIK NDVKPKLDLT PQVEYAALQT VVYPDKIVNA
FFGPIIKEIN ERIIRALRPH VVFNSRMTAD ELNETVAFLT PHKYRALEID FSKFDKSKTG
LHIKAVIGLY KLFGLDGLLK VLWEKSQYQT YVKDRNFGLE AYLLYQQKSG NCDTYGSNTW
SAALALLDCL PLEDAHFCVF GGDDSLILFD QGYIISDPCR QLAGTWNLEC KVFDFKYPAF
CGKFLLCIDG KYQFVPDAAK FITKLGRTDV RDVEVLSEIY ISINDNYKSY KDFKVLDALD
KALVDRYRSP YSAISALVSL CYHIFDFNKF KLLFNCEGKF VDKKLRKDFE W
