ID   RDRP_AQRVA              Reviewed;        1240 AA.
AC   Q8VA42;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   29-SEP-2021, entry version 56.
DE   RecName: Full=RNA-directed RNA polymerase VP2;
DE            EC=2.7.7.48;
GN   Name=S2;
OS   Aquareovirus A (isolate Chum salmon/Japan/CSRV/1981) (AQRV-A).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Aquareovirus.
OX   NCBI_TaxID=928295;
OH   NCBI_TaxID=8018; Oncorhynchus keta (Chum salmon) (Salmo keta).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3819694; DOI=10.1099/0022-1317-68-2-353;
RA   Winton J.R., Lannan C.N., Fryer J.L., Hedrick R.P., Meyers T.R.,
RA   Plumb J.A., Yamamoto T.;
RT   "Morphological and biochemical properties of four members of a novel group
RT   of reoviruses isolated from aquatic animals.";
RL   J. Gen. Virol. 68:353-364(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Rao S., Carner G.R., Chen W., Winton J.R.;
RT   "Complete genome sequence of the chum salmon virus.";
RL   Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in transcription
CC       and genome replication. Following infection, it catalyzes the synthesis
CC       of fully conservative plus strands. After core assembly, which consists
CC       in recruitment of one capped plus-strand for each genomic segments and
CC       polymerase complexes, the polymerase switches mode and catalyzes the
CC       synthesis of complementary minus-strands (By similarity).
CC       {ECO:0000255|PROSITE-ProRule:PRU00539}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AF418295; AAL31497.1; -; Genomic_RNA.
DR   RefSeq; YP_398630.1; NC_007583.1.
DR   SMR; Q8VA42; -.
DR   GeneID; 3773156; -.
DR   KEGG; vg:3773156; -.
DR   GO; GO:0019013; C:viral nucleocapsid; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR012915; RdRP_5.
DR   InterPro; IPR007097; RNA-dir_pol_reovirus.
DR   Pfam; PF07925; RdRP_5; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50523; RDRP_DSRNA_REO; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotide-binding; Nucleotidyltransferase;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..1240
FT                   /note="RNA-directed RNA polymerase VP2"
FT                   /id="PRO_0000404171"
FT   DOMAIN          516..764
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   1240 AA;  137575 MW;  7715D9DA5584D08D CRC64;
     MPGTYAPVPN VYHLLDHLTF RSSVVIPNSN FVGRIWSDYW ALQDNIVIRV SPEGAKDADY
     CHNSNISPIL SPLKTITEYG TLHPTIDKDA SERGYPSARM ASSFFKLASC QARQVKIDPT
     RFLEFLLVTA SSPRVPSGVD SDQPNPWLPE TSPALQAIWQ IMQRYKLNGN YYAPALVVNT
     GAVWWIPPPG RTNCVTVQFL ITDLINLAVN AFATRLAPEL EMCAVRVYLA AASTPNYAHA
     LLDLKSIFPN LSLHSMYREG EFGGKCPRIE WTEPRSSYRF KWIGVTQLYE GLRPLTPSRD
     SKALLKMRDS GLEDVAKVII TMRRDHPRHT SDSVRFVRGV LSLISGMYLV RPPTMSVLRE
     YSQTPQIEEP IPPDWWTGAV GNLSYFNDKA KGPLAHLYSV WLEAARQVVM DPSTHDPLTQ
     AIYKTQFVTP RGGSSAALKQ ALVESKVELP DFSGTGVKRS SKIYQTAQLA HFSFQTLIPT
     IMGQVTLGIR NQVQRRARSI MPMSNPQQTV SVPHTLVANY INKHMNRSTT SGSAVQDKVI
     PLLLYASTPP RTVINVDIKA CDASITYAAF LAPICGAMHQ GFDLGDPSLP FMNVPSSTQY
     DRRNPAAPYN RPVSGLQTMT QHLARLYQAG FSYKVDDPFS SGNSFVFPTT TFPSGSTATS
     TEHTANNGAM ADFFLREYVP QHAKSSTLKF IVKDMNIQNN YVCQGDDGML IIPDLGTKRI
     SPEDLAELME LLEKYGRGFG WVYDIDSSDS AEYLKLYALF GARIPNISRH PPVGKEYASP
     ETGEIWPSLV NIAMGSFYNG VTDCLEWRDW LKFSWAFACF ASRGSFHPKT GPRVDAQYPV
     WSFIYMGLPP ILLPGQTPFL TSVYMPAGDQ GIFAILHQWR DYLTARATSD YPPLKRRHPV
     WHLADVPSLL SDLGVYRGYW AAQVSRRPEP SPDDADPASV EAMSAALSTY LLKDPVLRDR
     VVRGTNAWRR LTDTHPGRLP SRVPSLLDVP TRWIKAGRDA DKPRPSAVAM MMKDIQRAAS
     SSRKDFSRLL ELYLHVHVHL GPPVPLAVDP EVPHVAGADI LNDDHWYKVT SLGPIAQSTK
     KYFDATLFVG KTVSGLDVEA VDATLLRMSI LGAEPEEYHA FLAGIGMSDA EAHRIASAIS
     LADAQIVQLA RTVNLAVPSS WMSLDFDTLI RSHSYPRQPG ISDSSTLVRE RASWINSVLR
     LLCATVAMSR VGPVCQATVS SVDGGVNQIV GCLRAWMRDV