ID   RCOM_RHORT              Reviewed;         236 AA.
AC   Q2RNI6;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=CO-responsive transcriptional regulator RcoM;
GN   Name=rcoM; OrderedLocusNames=Rru_A3515;
OS   Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
OS   NCIMB 8255 / S1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Rhodospirillum.
OX   NCBI_TaxID=269796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1;
RX   PubMed=21886856; DOI=10.4056/sigs.1804360;
RA   Munk A.C., Copeland A., Lucas S., Lapidus A., Del Rio T.G., Barry K.,
RA   Detter J.C., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D.,
RA   Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Kyrpides N.C.,
RA   Mavromatis K., Richardson P., Rohde M., Goeker M., Klenk H.P., Zhang Y.,
RA   Roberts G.P., Reslewic S., Schwartz D.C.;
RT   "Complete genome sequence of Rhodospirillum rubrum type strain (S1).";
RL   Stand. Genomic Sci. 4:293-302(2011).
RN   [2]
RP   FUNCTION AS A TRANSCRIPTIONAL ACTIVATOR, DISRUPTION PHENOTYPE, AND GENE
RP   NAME.
RX   PubMed=21115659; DOI=10.1128/jb.00794-10;
RA   Kerby R.L., Roberts G.P.;
RT   "Sustaining N2-dependent growth in the presence of CO.";
RL   J. Bacteriol. 193:774-777(2011).
CC   -!- FUNCTION: Activates the expression of the CowN protein in response to
CC       carbon monoxide (CO). Is required to sustain N(2)-dependent growth in
CC       the presence of low levels of carbon monoxide (CO).
CC       {ECO:0000269|PubMed:21115659}.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC       Note=Binds 1 heme group per subunit. {ECO:0000250};
CC   -!- DOMAIN: The N-terminal sensor (heme-containing) domain is covalently
CC       linked to the C-terminal, DNA-binding response domain. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Strains lacking this gene are not able to grow
CC       with N(2) as a nitrogen source in the presence of CO.
CC       {ECO:0000269|PubMed:21115659}.
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DR   EMBL; CP000230; ABC24309.1; -; Genomic_DNA.
DR   RefSeq; WP_011391262.1; NC_007643.1.
DR   RefSeq; YP_428596.1; NC_007643.1.
DR   AlphaFoldDB; Q2RNI6; -.
DR   SMR; Q2RNI6; -.
DR   STRING; 269796.Rru_A3515; -.
DR   EnsemblBacteria; ABC24309; ABC24309; Rru_A3515.
DR   KEGG; rru:Rru_A3515; -.
DR   PATRIC; fig|269796.9.peg.3632; -.
DR   eggNOG; COG3279; Bacteria.
DR   HOGENOM; CLU_097085_0_0_5; -.
DR   OMA; EGHYARA; -.
DR   OrthoDB; 1958442at2; -.
DR   PhylomeDB; Q2RNI6; -.
DR   Proteomes; UP000001929; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0006808; P:regulation of nitrogen utilization; IMP:UniProtKB.
DR   CDD; cd00130; PAS; 1.
DR   InterPro; IPR007492; LytTR_DNA-bd_dom.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   Pfam; PF04397; LytTR; 1.
DR   SMART; SM00850; LytTR; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   PROSITE; PS50930; HTH_LYTTR; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Heme; Iron; Metal-binding; Nitrogen fixation;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..236
FT                   /note="CO-responsive transcriptional regulator RcoM"
FT                   /id="PRO_0000407277"
FT   DOMAIN          1..64
FT                   /note="PAS"
FT   DOMAIN          131..236
FT                   /note="HTH LytTR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00112"
FT   BINDING         52
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   236 AA;  24974 MW;  732C9813648A1837 CRC64;
     MDDFAYNLRR AETGVLLLAD DLTVTAVSPG ALSLLGLDKP GALLGRPILD LHPPPLRPKV
     AVLLKTARGP SGPAATAVLS LRGGPVLIRA SALTGQGETA FALVLTAVGE SRETKEGPSA
     RPAPPGYLRK VPLGLGETTE FVDTAGVIYL EADGHYSRVH TAFGHSFCPL ALAELERRLD
     PDQFLRVHRS YIVALAHVRA FRKRESGGLL VMDTGAGDLV PIGRAQVTRL RGLLAI