ID   RCI2_ECOLX              Reviewed;         384 AA.
AC   P16470;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Shufflon-specific DNA recombinase;
GN   Name=rci;
OS   Escherichia coli.
OG   Plasmid IncI1 ColIb-P9.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2623084; DOI=10.1016/0147-619x(89)90029-2;
RA   Kim S.-R., Komano T.;
RT   "Cloning and nucleotide sequence of the ColIb shufflon.";
RL   Plasmid 22:180-184(1989).
CC   -!- FUNCTION: Shufflon-specific DNA recombinase.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; D90039; BAA14093.1; -; Genomic_DNA.
DR   RefSeq; WP_001139958.1; NZ_WVUW01000022.1.
DR   RefSeq; YP_006903584.1; NC_019043.1.
DR   RefSeq; YP_009061281.1; NC_024976.1.
DR   RefSeq; YP_009061514.1; NC_024978.1.
DR   RefSeq; YP_009061753.1; NC_024980.1.
DR   RefSeq; YP_009069675.1; NC_025147.1.
DR   RefSeq; YP_009071287.1; NC_025180.1.
DR   AlphaFoldDB; P16470; -.
DR   SMR; P16470; -.
DR   GeneID; 39515077; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR035069; TTHA1013/TTHA0281-like.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF143100; SSF143100; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid.
FT   CHAIN           1..384
FT                   /note="Shufflon-specific DNA recombinase"
FT                   /id="PRO_0000197536"
FT   DOMAIN          9..96
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          118..284
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        155
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        235
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        238
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        271
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   384 AA;  44253 MW;  DF915524907FA71E CRC64;
     MPSPRIRKMS LSRALDKYLK TVSVHKKGHQ QEFYRSNVIK RYPIALRNMD EITTVDIATY
     RDVRLAEINP RTGKPITGNT VRLELALLSS LFNIARVEWG TCRTNPVELV RKPKVSSGRD
     RRLTSSEERR LSRYFREKNL MLYVIFHLAL ETAMRQGEIL ALRWEHIDLR HGVAHLPETK
     NGHSRDVPLS RRARNFLQMM PVNLHGNVFD YTASGFKNAW RIATQRLRIE DLHFHDLRHE
     AISRFFELGS LNVMEIAAIS GHRSMNMLKR YTHLRAWQLV SKLDARRRQT QKVAAWFVPY
     PAHITTINEE NGQKAHRIEI GDFDNLHVTA TTKEEAVHRA SEVLLRTLAI AAQKGERVPS
     PGALPVNDPD YIMICPLNPG STPL