RAP2C_BOVIN
ID RAP2C_BOVIN Reviewed; 183 AA.
AC Q08DI5;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Ras-related protein Rap-2c;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P10114};
DE Flags: Precursor;
GN Name=RAP2C;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal muscle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Small GTP-binding protein which cycles between a GDP-bound
CC inactive and a GTP-bound active form. May play a role in cytoskeletal
CC rearrangements and regulate cell spreading through activation of the
CC effector TNIK. May play a role in SRE-mediated gene transcription (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P10114};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Recycling endosome
CC membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}.
CC -!- PTM: Palmitoylated. Palmitoylation is required for association with
CC recycling endosome membranes and activation of TNIK.
CC {ECO:0000250|UniProtKB:Q8BU31}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000305}.
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DR EMBL; BC123730; AAI23731.1; -; mRNA.
DR RefSeq; NP_001069168.1; NM_001075700.1.
DR RefSeq; XP_005227569.1; XM_005227512.3.
DR RefSeq; XP_005227570.1; XM_005227513.3.
DR RefSeq; XP_010819712.1; XM_010821410.2.
DR AlphaFoldDB; Q08DI5; -.
DR SMR; Q08DI5; -.
DR STRING; 9913.ENSBTAP00000005408; -.
DR PaxDb; Q08DI5; -.
DR PRIDE; Q08DI5; -.
DR Ensembl; ENSBTAT00000005408; ENSBTAP00000005408; ENSBTAG00000004131.
DR Ensembl; ENSBTAT00000068302; ENSBTAP00000061865; ENSBTAG00000004131.
DR Ensembl; ENSBTAT00000079003; ENSBTAP00000066215; ENSBTAG00000004131.
DR Ensembl; ENSBTAT00000080153; ENSBTAP00000059791; ENSBTAG00000004131.
DR GeneID; 515181; -.
DR KEGG; bta:515181; -.
DR CTD; 57826; -.
DR VEuPathDB; HostDB:ENSBTAG00000004131; -.
DR VGNC; VGNC:33721; RAP2C.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000157245; -.
DR HOGENOM; CLU_041217_9_8_1; -.
DR InParanoid; Q08DI5; -.
DR OMA; QFTGINE; -.
DR OrthoDB; 1353024at2759; -.
DR TreeFam; TF313014; -.
DR Proteomes; UP000009136; Chromosome X.
DR Bgee; ENSBTAG00000004131; Expressed in caput epididymis and 105 other tissues.
DR ExpressionAtlas; Q08DI5; baseline and differential.
DR GO; GO:0005923; C:bicellular tight junction; IEA:Ensembl.
DR GO; GO:0044291; C:cell-cell contact zone; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0019003; F:GDP binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IEA:Ensembl.
DR GO; GO:0090557; P:establishment of endothelial intestinal barrier; IEA:Ensembl.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0031954; P:positive regulation of protein autophosphorylation; ISS:UniProtKB.
DR GO; GO:0032486; P:Rap protein signal transduction; ISS:UniProtKB.
DR GO; GO:0061097; P:regulation of protein tyrosine kinase activity; ISS:UniProtKB.
DR CDD; cd04176; Rap2; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041840; Rap2.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; GTP-binding; Hydrolase; Lipoprotein; Membrane;
KW Methylation; Nucleotide-binding; Palmitate; Prenylation;
KW Reference proteome.
FT CHAIN 1..180
FT /note="Ras-related protein Rap-2c"
FT /id="PRO_0000280802"
FT PROPEP 181..183
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:Q8BU31"
FT /id="PRO_0000280803"
FT MOTIF 32..40
FT /note="Effector region"
FT /evidence="ECO:0000305"
FT BINDING 10..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 57..61
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 116..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 180
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:Q8BU31"
FT LIPID 176
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q8BU31"
FT LIPID 177
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q8BU31"
FT LIPID 180
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q8BU31"
SQ SEQUENCE 183 AA; 20745 MW; 6763385F76638324 CRC64;
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG
TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI VRVKRYEKVP LILVGNKVDL
EPEREVMSSE GRALAQEWGC PFMETSAKSK SMVDELFAEI VRQMNYSSLP EKQDQCCTTC
VVQ
