RACE_DICDI
ID RACE_DICDI Reviewed; 223 AA.
AC Q23862; Q54U41;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Rho-related protein racE;
DE Flags: Precursor;
GN Name=racE; ORFNames=DDB_G0280975;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX3 / DH1;
RX PubMed=8682867; DOI=10.1083/jcb.133.6.1321;
RA Larochelle D.A., Vithalani K.K., de Lozanne A.;
RT "A novel member of the rho family of small GTP-binding proteins is
RT specifically required for cytokinesis.";
RL J. Cell Biol. 133:1321-1330(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=11222756; DOI=10.1093/nar/29.5.1068;
RA Rivero F., Dislich H., Gloeckner G., Noegel A.A.;
RT "The Dictyostelium discoideum family of Rho-related proteins.";
RL Nucleic Acids Res. 29:1068-1079(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP INTERACTION WITH RGAA.
RX PubMed=9739079; DOI=10.1242/jcs.111.20.3059;
RA Faix J., Clougherty C., Konzok A., Mintert U., Murphy J., Albrecht R.,
RA Muhlbauer B., Kuhlmann J.;
RT "The IQGAP-related protein DGAP1 interacts with Rac and is involved in the
RT modulation of the F-actin cytoskeleton and control of cell motility.";
RL J. Cell Sci. 111:3059-3071(1998).
CC -!- FUNCTION: Specifically required for cytokinesis.
CC -!- SUBUNIT: Interacts with rgaA. {ECO:0000269|PubMed:9739079}.
CC -!- INTERACTION:
CC Q23862; O76187: darA; NbExp=2; IntAct=EBI-2118748, EBI-2889579;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
CC {ECO:0000305}.
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DR EMBL; U41222; AAB16752.1; -; Genomic_DNA.
DR EMBL; AF310890; AAG45124.1; -; Genomic_DNA.
DR EMBL; AAFI02000040; EAL66784.1; -; Genomic_DNA.
DR RefSeq; XP_640935.1; XM_635843.1.
DR AlphaFoldDB; Q23862; -.
DR SMR; Q23862; -.
DR IntAct; Q23862; 2.
DR STRING; 44689.DDB0214825; -.
DR PaxDb; Q23862; -.
DR EnsemblProtists; EAL66784; EAL66784; DDB_G0280975.
DR GeneID; 8623000; -.
DR KEGG; ddi:DDB_G0280975; -.
DR dictyBase; DDB_G0280975; racE.
DR eggNOG; KOG0393; Eukaryota.
DR HOGENOM; CLU_041217_21_1_1; -.
DR InParanoid; Q23862; -.
DR OMA; APEVNHY; -.
DR PhylomeDB; Q23862; -.
DR Reactome; R-DDI-9013406; RHOQ GTPase cycle.
DR Reactome; R-DDI-9013407; RHOH GTPase cycle.
DR Reactome; R-DDI-9013409; RHOJ GTPase cycle.
DR Reactome; R-DDI-9013418; RHOBTB2 GTPase cycle.
DR Reactome; R-DDI-9013422; RHOBTB1 GTPase cycle.
DR Reactome; R-DDI-9706019; RHOBTB3 ATPase cycle.
DR PRO; PR:Q23862; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0042995; C:cell projection; IBA:GO_Central.
DR GO; GO:0031254; C:cell trailing edge; IDA:dictyBase.
DR GO; GO:0032154; C:cleavage furrow; IDA:dictyBase.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0035591; F:signaling adaptor activity; IDA:dictyBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0019954; P:asexual reproduction; IMP:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0030865; P:cortical cytoskeleton organization; IGI:dictyBase.
DR GO; GO:0043652; P:engulfment of apoptotic cell; IBA:GO_Central.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0032467; P:positive regulation of cytokinesis; IGI:dictyBase.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling; IMP:dictyBase.
DR GO; GO:1904515; P:positive regulation of TORC2 signaling; IDA:dictyBase.
DR GO; GO:0051291; P:protein heterooligomerization; IDA:dictyBase.
DR GO; GO:0051260; P:protein homooligomerization; IDA:dictyBase.
DR GO; GO:0072697; P:protein localization to cell cortex; IMP:dictyBase.
DR GO; GO:0016601; P:Rac protein signal transduction; IMP:dictyBase.
DR GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0008360; P:regulation of cell shape; IBA:GO_Central.
DR GO; GO:0061118; P:regulation of positive chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0019953; P:sexual reproduction; IEP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003578; Small_GTPase_Rho.
DR PANTHER; PTHR24072; PTHR24072; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51420; RHO; 1.
PE 1: Evidence at protein level;
KW Cell membrane; GTP-binding; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..220
FT /note="Rho-related protein racE"
FT /id="PRO_0000198902"
FT PROPEP 221..223
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000281251"
FT REGION 187..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 40..48
FT /note="Effector region"
FT /evidence="ECO:0000255"
FT BINDING 18..25
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 65..69
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 123..126
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 220
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 220
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 223 AA; 24373 MW; 07760B3483237EE4 CRC64;
MSEDQGSGAT RVKLVVVGDG AVGKTCLLIC YAQNDFPVDY VPTVFENYTA TRKRGNEDIK
VHLWDTAGQE EYDRLRPLSY PGADVVLLCF STISQSSYEA IRDKWAPEVN HYIPDVPSIL
VGTKIDLREQ QHPDPNSGKF EPITADMGIS MQKQIKAKKY LEVSAKTRQG LEEVFSAAIE
IVLESRGMDK KSQDGSSSAS GVPSGDKPTK GKAGKKKSGC IIL
