RAC5_ORYSJ
ID RAC5_ORYSJ Reviewed; 197 AA.
AC Q6EP31; B7EDK5; Q9M628; Q9SLP0;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Rac-like GTP-binding protein 5;
DE AltName: Full=GTPase protein RacD;
DE AltName: Full=OsRac5;
DE Flags: Precursor;
GN Name=RAC5; Synonyms=RACD; OrderedLocusNames=Os02g0834000, LOC_Os02g58730;
GN ORFNames=OJ1282_E10.23-1, OJ1282_E10.23-2,
GN OsJ_09018 {ECO:0000312|EMBL:EEE58123.1}, P0264G11.2-1, P0264G11.2-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RA Mi Z., Wang S., Wu N.;
RT "Molecular cloning of small GTP binding protein gene osRACD gene from
RT rice.";
RL Chin. Sci. Bull. 45:2047-2054(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 154-197.
RA Wu N., Mi Z.;
RT "Rice small GTP binding protein.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NOMENCLATURE.
RX PubMed=16172097; DOI=10.1104/pp.105.063933;
RA Miki D., Itoh R., Shimamoto K.;
RT "RNA silencing of single and multiple members in a gene family of rice.";
RL Plant Physiol. 138:1903-1913(2005).
CC -!- FUNCTION: Inactive GDP-bound Rho GTPases reside in the cytosol, are
CC found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and
CC are released from the GDI protein in order to translocate to membranes
CC upon activation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Note=Associated with the
CC membrane when activated.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6EP31-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6EP31-2; Sequence=VSP_017559;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
CC {ECO:0000305}.
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DR EMBL; AF218381; AAF28764.1; -; mRNA.
DR EMBL; AF329814; AAK27450.1; -; Genomic_DNA.
DR EMBL; AP005290; BAD28462.1; -; Genomic_DNA.
DR EMBL; AP005290; BAD28463.1; -; Genomic_DNA.
DR EMBL; AP006187; BAD29588.1; -; Genomic_DNA.
DR EMBL; AP006187; BAD29589.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81793.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81794.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE58123.1; -; Genomic_DNA.
DR EMBL; AK067504; BAG90452.1; -; mRNA.
DR EMBL; AK067796; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AB036062; BAA88573.1; -; mRNA.
DR RefSeq; XP_015627011.1; XM_015771525.1. [Q6EP31-1]
DR AlphaFoldDB; Q6EP31; -.
DR SMR; Q6EP31; -.
DR STRING; 4530.OS02T0834000-01; -.
DR PaxDb; Q6EP31; -.
DR PRIDE; Q6EP31; -.
DR EnsemblPlants; Os02t0834000-01; Os02t0834000-01; Os02g0834000. [Q6EP31-1]
DR EnsemblPlants; Os02t0834000-02; Os02t0834000-02; Os02g0834000. [Q6EP31-2]
DR GeneID; 4331272; -.
DR Gramene; Os02t0834000-01; Os02t0834000-01; Os02g0834000. [Q6EP31-1]
DR Gramene; Os02t0834000-02; Os02t0834000-02; Os02g0834000. [Q6EP31-2]
DR KEGG; osa:4331272; -.
DR eggNOG; KOG0393; Eukaryota.
DR HOGENOM; CLU_041217_21_3_1; -.
DR InParanoid; Q6EP31; -.
DR OMA; SPRRHKC; -.
DR OrthoDB; 1091615at2759; -.
DR PlantReactome; R-OSA-5608118; Auxin signalling.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR ExpressionAtlas; Q6EP31; baseline and differential.
DR Genevisible; Q6EP31; OS.
DR GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR GO; GO:0042995; C:cell projection; IBA:GO_Central.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0030865; P:cortical cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0008360; P:regulation of cell shape; IBA:GO_Central.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003578; Small_GTPase_Rho.
DR PANTHER; PTHR24072; PTHR24072; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51420; RHO; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; GTP-binding; Lipoprotein; Membrane;
KW Methylation; Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..194
FT /note="Rac-like GTP-binding protein 5"
FT /id="PRO_0000227574"
FT PROPEP 195..197
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000227575"
FT MOTIF 35..43
FT /note="Effector region"
FT /evidence="ECO:0000255"
FT BINDING 13..20
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 60..64
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 118..121
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 194
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000255"
FT LIPID 194
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 63..64
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_017559"
SQ SEQUENCE 197 AA; 21591 MW; 1A7D4907388D9DC2 CRC64;
MSASRFIKCV TVGDGAVGKT CMLISYTSNT FPTDYVPTVF DNFSANVVVD GSTVNLGLWD
TAGQEDYNRL RPLSYRGADV FLLAFSLISK ASYENVSKKW IPELRHYAPG VPIILVGTKL
DLRDDKQFFV DHPGAVPIST AQGEELRKLI GAAAYIECSS KTQQNIKAVF DAAIKVVLQP
PKQKKKKKKA QKGCAIL
