RAB1B_DICDI
ID RAB1B_DICDI Reviewed; 206 AA.
AC P34140; Q54Y93;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ras-related protein Rab-1B;
DE Flags: Precursor;
GN Name=rab1B; ORFNames=DDB_G0277867;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-206.
RC STRAIN=AX3;
RX PubMed=8294041; DOI=10.1016/0378-1119(93)90447-b;
RA Bush J.M. IV, Franek K., Daniel J.M., Spiegelman G.B., Weeks G.,
RA Cardelli J.A.;
RT "Cloning and characterization of five novel Dictyostelium discoideum rab-
RT related genes.";
RL Gene 136:55-60(1993).
CC -!- FUNCTION: The small GTPases Rab are key regulators of intracellular
CC membrane trafficking, from the formation of transport vesicles to their
CC fusion with membranes. Rabs cycle between an inactive GDP-bound form
CC and an active GTP-bound form that is able to recruit to membranes
CC different set of downstream effectors directly responsible for vesicle
CC formation, movement, tethering and fusion. Rab1B regulates vesicular
CC transport between the endoplasmic reticulum and successive Golgi
CC compartments. Plays a role in the initial events of the autophagic
CC vacuole development which take place at specialized regions of the
CC endoplasmic reticulum. {ECO:0000250|UniProtKB:Q9H0U4}.
CC -!- ACTIVITY REGULATION: Rab activation is generally mediated by a guanine
CC exchange factor (GEF), while inactivation through hydrolysis of bound
CC GTP is catalyzed by a GTPase activating protein (GAP). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9H0U4}.
CC Membrane {ECO:0000250|UniProtKB:Q9H0U4}; Lipid-anchor
CC {ECO:0000250|UniProtKB:Q9H0U4}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q9H0U4}. Preautophagosomal structure membrane
CC {ECO:0000250|UniProtKB:Q9H0U4}; Lipid-anchor {ECO:0000305}; Cytoplasmic
CC side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000023; EAL68106.1; -; Genomic_DNA.
DR EMBL; L21010; AAC37386.1; -; mRNA.
DR RefSeq; XP_642309.1; XM_637217.1.
DR AlphaFoldDB; P34140; -.
DR SMR; P34140; -.
DR STRING; 44689.DDB0214820; -.
DR PaxDb; P34140; -.
DR PRIDE; P34140; -.
DR EnsemblProtists; EAL68106; EAL68106; DDB_G0277867.
DR GeneID; 8621515; -.
DR KEGG; ddi:DDB_G0277867; -.
DR dictyBase; DDB_G0277867; rab1B.
DR eggNOG; KOG0084; Eukaryota.
DR HOGENOM; CLU_041217_10_1_1; -.
DR InParanoid; P34140; -.
DR OMA; QRYACDS; -.
DR PhylomeDB; P34140; -.
DR Reactome; R-DDI-162658; Golgi Cisternae Pericentriolar Stack Reorganization.
DR Reactome; R-DDI-204005; COPII-mediated vesicle transport.
DR Reactome; R-DDI-6807878; COPI-mediated anterograde transport.
DR Reactome; R-DDI-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-DDI-6811440; Retrograde transport at the Trans-Golgi-Network.
DR Reactome; R-DDI-8873719; RAB geranylgeranylation.
DR Reactome; R-DDI-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:P34140; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005811; C:lipid droplet; HDA:dictyBase.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Cytoplasm; GTP-binding; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..203
FT /note="Ras-related protein Rab-1B"
FT /id="PRO_0000121267"
FT PROPEP 204..206
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000370753"
FT MOTIF 37..45
FT /note="Effector region"
FT /evidence="ECO:0000250|UniProtKB:Q9H0U4"
FT BINDING 15..23
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62820"
FT BINDING 33..40
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62820"
FT BINDING 63..67
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9H0U4"
FT BINDING 121..124
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q9H0U4"
FT BINDING 151..153
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62820"
FT MOD_RES 203
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000255"
FT LIPID 203
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9H0U4"
FT CONFLICT 67
FT /note="Missing (in Ref. 2; AAC37386)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="L -> K (in Ref. 2; AAC37386)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 206 AA; 23126 MW; BCB16A3C6EC56ABF CRC64;
MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI NLDGKIIKLQ
IWDTAGQERF RTITSSYYRG AHGIIVVYDV TDHVSFNNVK QWMQEIQRYA CDSVTRLLVG
NKCDLIEKKI VDTSTAREYA DSVGIPFLET SAKSSANVEQ AFMIMASEIK KLQGGIQPNN
NSTYNAHVVK PTGFTPIGKK KKCSII
