ID   PWAPL_LOTGI             Reviewed;         225 AA.
AC   B3A0S1;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Perlwapin-like protein;
DE   Flags: Precursor; Fragment;
OS   Lottia gigantea (Giant owl limpet).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX   NCBI_TaxID=225164;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|Ref.1};
RA   Richardson P., Lucas S., Rokhsar D., Wang M., Lindquist E.A.;
RT   "DOE Joint Genome Institute Lottia gigantea EST project.";
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 117-131, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23145877; DOI=10.1111/febs.12062;
RA   Marie B., Jackson D.J., Ramos-Silva P., Zanella-Cleon I., Guichard N.,
RA   Marin F.;
RT   "The shell-forming proteome of Lottia gigantea reveals both deep
RT   conservations and lineage-specific novelties.";
RL   FEBS J. 280:214-232(2013).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23145877}.
CC   -!- TISSUE SPECIFICITY: Component of the acid-soluble organic matrix of
CC       calcified layers of the shell (at protein level).
CC       {ECO:0000269|PubMed:23145877}.
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DR   EMBL; FC634663; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; B3A0S1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030414; F:peptidase inhibitor activity; IEA:InterPro.
DR   InterPro; IPR008197; WAP_dom.
DR   PROSITE; PS51390; WAP; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..225
FT                   /note="Perlwapin-like protein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000415260"
FT   DOMAIN          27..68
FT                   /note="WAP 1; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DOMAIN          117..169
FT                   /note="WAP 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT   REGION          176..225
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        176..198
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        170
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        27..57
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        36..61
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        43..56
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        49..65
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        125..157
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        135..161
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        143..156
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   DISULFID        149..165
FT                   /evidence="ECO:0000250|UniProtKB:Q9N0L8,
FT                   ECO:0000255|PROSITE-ProRule:PRU00722"
FT   NON_TER         225
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   225 AA;  24775 MW;  F7D6CE7F2B96CDED CRC64;
     MNHLWLFIVT VSCIYLVYGQ GEESVPCKVK FMGTACPLGR LVCEEDGDCL GVNQVCCYDG
     CGTTCHNKTT TLIPSTTQPQ IQPIIPSTQP QIQPIIPSTQ PQIQPFIPST QPQIQRIIPS
     PELLCPVVTV RYAFCRFSTY TPCHTSNDCA VPGMKCCPDV CGKRCKFPIN STDHQQFQQT
     PLKPTVPLPQ YQQTPLQPTV PSSQPPLQPT VPSPQSYNYK GACST