PUX7_ARATH
ID PUX7_ARATH Reviewed; 468 AA.
AC Q94JZ8; Q9MA26;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 143.
DE RecName: Full=Plant UBX domain-containing protein 7 {ECO:0000303|Ref.5};
DE Short=PUX7 {ECO:0000303|Ref.5};
GN Name=PUX7 {ECO:0000303|Ref.5};
GN OrderedLocusNames=At1g14570 {ECO:0000312|Araport:AT1G14570};
GN ORFNames=T5E21.7 {ECO:0000312|EMBL:AAF63167.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY.
RA Posthuma R., Rancour D., Park S., Bates B., Bednarek S.;
RT "The plant UBX-domain containing (PUX) protein family regulates the
RT function of Arabidopsis CDC48, a conserved essential AAA-ATPase.";
RL (In) Proceedings of the 16th international conference on Arabidopsis
RL research, abstract#116, Madison (2005).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [7]
RP INTERACTION WITH CDC48A AND UBIQUITIN, SUBCELLULAR LOCATION, FUNCTION,
RP DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=23747397; DOI=10.1016/j.gene.2013.05.056;
RA Gallois J.L., Drouaud J., Lecureuil A., Guyon-Debast A., Bonhomme S.,
RA Guerche P.;
RT "Functional characterization of the plant ubiquitin regulatory X (UBX)
RT domain-containing protein AtPUX7 in Arabidopsis thaliana.";
RL Gene 526:299-308(2013).
CC -!- FUNCTION: Acts as a bridge between CDC48A and ubiquitin, suggesting a
CC role in targeted protein degradation. {ECO:0000269|PubMed:23747397}.
CC -!- SUBUNIT: Interacts with CDC48A via its UBX domain and with ubiquitin
CC via its N-terminal UBA-like domain. {ECO:0000269|PubMed:23747397}.
CC -!- INTERACTION:
CC Q94JZ8; P54609: CDC48A; NbExp=3; IntAct=EBI-10684297, EBI-1563238;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23747397}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q94JZ8-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed broadly in sporophyte and gametophyte
CC cells. {ECO:0000269|PubMed:23747397}.
CC -!- DEVELOPMENTAL STAGE: Expressed early during male gametophyte
CC development. {ECO:0000269|PubMed:23747397}.
CC -!- DOMAIN: The UIM (ubiquitin-interacting motif) is required to engage the
CC NEDD8 modification on cullins. {ECO:0000250|UniProtKB:O94888}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:23747397}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF63167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010657; AAF63167.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29182.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29183.1; -; Genomic_DNA.
DR EMBL; AF370507; AAK43884.1; -; mRNA.
DR EMBL; AY064683; AAL47388.1; -; mRNA.
DR EMBL; AK317323; BAH19997.1; -; mRNA.
DR PIR; D86280; D86280.
DR RefSeq; NP_563954.1; NM_101324.4. [Q94JZ8-1]
DR RefSeq; NP_973827.1; NM_202098.3. [Q94JZ8-1]
DR AlphaFoldDB; Q94JZ8; -.
DR SMR; Q94JZ8; -.
DR IntAct; Q94JZ8; 1.
DR STRING; 3702.AT1G14570.2; -.
DR TCDB; 3.A.16.1.5; the endoplasmic reticular retrotranslocon (er-rt) family.
DR iPTMnet; Q94JZ8; -.
DR PaxDb; Q94JZ8; -.
DR PRIDE; Q94JZ8; -.
DR ProteomicsDB; 236548; -. [Q94JZ8-1]
DR EnsemblPlants; AT1G14570.1; AT1G14570.1; AT1G14570. [Q94JZ8-1]
DR EnsemblPlants; AT1G14570.2; AT1G14570.2; AT1G14570. [Q94JZ8-1]
DR GeneID; 838019; -.
DR Gramene; AT1G14570.1; AT1G14570.1; AT1G14570. [Q94JZ8-1]
DR Gramene; AT1G14570.2; AT1G14570.2; AT1G14570. [Q94JZ8-1]
DR KEGG; ath:AT1G14570; -.
DR Araport; AT1G14570; -.
DR TAIR; locus:2204497; AT1G14570.
DR eggNOG; KOG1364; Eukaryota.
DR InParanoid; Q94JZ8; -.
DR OMA; PAIFDCQ; -.
DR OrthoDB; 1130151at2759; -.
DR PhylomeDB; Q94JZ8; -.
DR PRO; PR:Q94JZ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94JZ8; baseline and differential.
DR Genevisible; Q94JZ8; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IDA:TAIR.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR006577; UAS.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR017346; UBX_7/2.
DR InterPro; IPR001012; UBX_dom.
DR InterPro; IPR003903; UIM_dom.
DR Pfam; PF00789; UBX; 1.
DR PIRSF; PIRSF037991; UCP037991_UBX7/2; 1.
DR SMART; SM00594; UAS; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50033; UBX; 1.
DR PROSITE; PS50330; UIM; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Nucleus; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..468
FT /note="Plant UBX domain-containing protein 7"
FT /id="PRO_0000432605"
FT DOMAIN 7..48
FT /note="UBA-like"
FT DOMAIN 328..347
FT /note="UIM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00213"
FT DOMAIN 385..466
FT /note="UBX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00215"
FT REGION 138..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..168
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 468 AA; 51808 MW; 5E81A39E11B31F35 CRC64;
MEGMLSSGDQ QRLVSSFLEI AVGQTAETAR QFLQATSWKL EEAIQLFYIG NEGGMLQSGT
HTQPASNDDA AAQSWGAATG TGNEMILPND VDEVRAPLPV VRETLYGESM YYGAMRVGNS
QPEPNSLIAF RNFSEEPKSP GIWEPDEGDS SASASASASA SESASAPRDS LASLYRPPFH
LMFQGSFEQA KTTSSSQDKW LLVNLQSTTE FSSHMLNRDT WANDAVSQTI KANFIFWQVY
DDTTEGRKVC TYYKLESIPV VLVIDPTTGQ RMRMWTGMVD PENLLEDLVP FMDGGPREHF
ASLSKKRPRG SFSLTPHSKP KEDVAKDEEE EELQRALAAS LEDNNMKESS DDQSTIIPEE
VAVEAVTSAV LPTFPPLPEE PKGGDRSLQC RVGIRLPNGQ RLQRNFLKTD TIQLLWSFCY
SQLEESERKK PLKLTQAIPG ESKTLEYESN LTLEQSGVAN SMISATWE
