ID   PUTR_BACSU              Reviewed;         411 AA.
AC   P94393;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Proline-responsive transcriptional activator PutR {ECO:0000303|PubMed:21840319};
DE   AltName: Full=Proline catabolism regulator {ECO:0000303|PubMed:21964733};
GN   Name=putR {ECO:0000303|PubMed:21840319};
GN   Synonyms=prcR {ECO:0000303|PubMed:21964733}, ycgP;
GN   OrderedLocusNames=BSU03230;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 355.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   FUNCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RX   PubMed=21840319; DOI=10.1016/j.jmb.2011.08.003;
RA   Belitsky B.R.;
RT   "Indirect repression by Bacillus subtilis CodY via displacement of the
RT   activator of the proline utilization operon.";
RL   J. Mol. Biol. 413:321-336(2011).
RN   [5]
RP   FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE, AND INDUCTION.
RC   STRAIN=168;
RX   PubMed=21964733; DOI=10.1099/mic.0.054197-0;
RA   Huang S.C., Lin T.H., Shaw G.C.;
RT   "PrcR, a PucR-type transcriptional activator, is essential for proline
RT   utilization and mediates proline-responsive expression of the proline
RT   utilization operon putBCP in Bacillus subtilis.";
RL   Microbiology 157:3370-3377(2011).
RN   [6]
RP   FUNCTION.
RC   STRAIN=168 / JH642;
RX   PubMed=22139509; DOI=10.1128/jb.06380-11;
RA   Moses S., Sinner T., Zaprasis A., Stoeveken N., Hoffmann T., Belitsky B.R.,
RA   Sonenshein A.L., Bremer E.;
RT   "Proline utilization by Bacillus subtilis: uptake and catabolism.";
RL   J. Bacteriol. 194:745-758(2012).
CC   -!- FUNCTION: Activates transcription of the putBCP operon. Requires
CC       proline as a coactivator. {ECO:0000269|PubMed:21840319,
CC       ECO:0000269|PubMed:21964733, ECO:0000269|PubMed:22139509}.
CC   -!- INDUCTION: PrcR expression is not subject to autoregulation and proline
CC       induction. {ECO:0000269|PubMed:21964733}.
CC   -!- DISRUPTION PHENOTYPE: Mutant loses the ability to utilize proline as
CC       either sole nitrogen or sole carbon source.
CC       {ECO:0000269|PubMed:21840319, ECO:0000269|PubMed:21964733}.
CC   -!- SIMILARITY: Belongs to the CdaR family. {ECO:0000305}.
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DR   EMBL; D50453; BAA08957.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12117.2; -; Genomic_DNA.
DR   PIR; C69759; C69759.
DR   RefSeq; NP_388205.2; NC_000964.3.
DR   RefSeq; WP_003234651.1; NZ_JNCM01000030.1.
DR   AlphaFoldDB; P94393; -.
DR   SMR; P94393; -.
DR   STRING; 224308.BSU03230; -.
DR   PaxDb; P94393; -.
DR   PRIDE; P94393; -.
DR   DNASU; 938337; -.
DR   EnsemblBacteria; CAB12117; CAB12117; BSU_03230.
DR   GeneID; 938337; -.
DR   KEGG; bsu:BSU03230; -.
DR   PATRIC; fig|224308.179.peg.337; -.
DR   eggNOG; COG2508; Bacteria.
DR   InParanoid; P94393; -.
DR   OMA; ALCTVPW; -.
DR   PhylomeDB; P94393; -.
DR   BioCyc; BSUB:BSU03230-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.2840; -; 1.
DR   InterPro; IPR041522; CdaR_GGDEF.
DR   InterPro; IPR025736; PucR_C-HTH_dom.
DR   InterPro; IPR042070; PucR_C-HTH_sf.
DR   Pfam; PF17853; GGDEF_2; 1.
DR   Pfam; PF13556; HTH_30; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..411
FT                   /note="Proline-responsive transcriptional activator PutR"
FT                   /id="PRO_0000165947"
FT   CONFLICT        355
FT                   /note="I -> V (in Ref. 1; BAA08957)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   411 AA;  47844 MW;  1947A428FC28E643 CRC64;
     MEELLERVFS FSDVDKLIDF ISYELQKPVI LESADFFLLA YNSYYINHFD SANQQTIFSK
     KCPVQIFERF LKDGIIEKLK TEPEPFRVNK IESIGLNQRV VVSAKHKGEV MGYIWIQELD
     QNLTDEELDF LYETSFHVGK IIYKTNKLKQ EKEEKAEDLI KRAIYQQFTS EKELRREAER
     INTVLPSMFS VVILHAANGD GEAVEDLKEN IRSYLNLRDK VSHVLTIESN IVIVVASFSQ
     KSSVSSAASE FINKLLTHFH FQKIPTPIYI GIGNEYNHLL KLGKSYTEAL EVIKAAEITG
     NQENIPYEYA KLGIYRYLES IEQKNEFLEY ENKDLALLKA KDEESSTELL KTLEIYLLNN
     CKTKPAAEQL FIHQNTLNYR IKQITEMTSI DLSDFRTRCQ LYLDLMLMKK K