ID   PUTP_KLEOX              Reviewed;         160 AA.
AC   P23723;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Sodium/proline symporter {ECO:0000250|UniProtKB:P07117};
DE   AltName: Full=Proline permease;
DE   Flags: Fragment;
GN   Name=putP {ECO:0000303|PubMed:1987164};
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=M5a1;
RX   PubMed=8282188; DOI=10.1111/j.1574-6968.1993.tb06572.x;
RA   Suhr M., Kleiner D.;
RT   "Genetic analysis of the regulatory putP region (coding for proline
RT   permease) in Klebsiella pneumoniae M5a1: evidence for regulation by the nac
RT   system.";
RL   FEMS Microbiol. Lett. 114:191-194(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
RX   PubMed=1987164; DOI=10.1128/jb.173.2.783-790.1991;
RA   Chen L.M., Maloy S.;
RT   "Regulation of proline utilization in enteric bacteria: cloning and
RT   characterization of the Klebsiella put control region.";
RL   J. Bacteriol. 173:783-790(1991).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P07117}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Expression may be regulated by the Nac regulatory protein
CC       and the nitrogen regulation (Ntr) system. {ECO:0000303|PubMed:8282188}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; X74934; CAA52889.1; -; Genomic_DNA.
DR   EMBL; M63160; AAA25138.1; -; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid transport; Cell inner membrane; Cell membrane; Ion transport;
KW   Membrane; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..>160
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000105400"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   NON_TER         160
SQ   SEQUENCE   160 AA;  17786 MW;  7EDB644EDA750729 CRC64;
     MAISTPMLVT FIVYIFGMVL IGFIAWRSTK NFDDYILGGR SLGPFVTALS AGASDMSGWL
     LMGLPGAIFI SGISESWIAI GLTLGAWINW KLVAGRLRVH TEVNNNALTX PDYFTGRFED
     HHNFHGFRTR VVKHIACILY RHDGALCGIG SRHAQRHFLH