PSAF_ARATH
ID PSAF_ARATH Reviewed; 221 AA.
AC Q9SHE8; Q941C1; Q9SUI8;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Photosystem I reaction center subunit III, chloroplastic;
DE AltName: Full=Light-harvesting complex I 17 kDa protein;
DE AltName: Full=PSI-F;
DE Flags: Precursor;
GN Name=PSAF; OrderedLocusNames=At1g31330; ORFNames=T19E23.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Legen J., Misera S., Herrmann R.G., Altschmied L.;
RT "Sequences and map position of 31 Arabidopsis thaliana cDNAs encoding
RT organellar polypeptides.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=10900198; DOI=10.1074/jbc.m002933200;
RA Haldrup A., Simpson D.J., Scheller H.V.;
RT "Down-regulation of the PSI-F subunit of photosystem I (PSI) in Arabidopsis
RT thaliana. The PSI-F subunit is essential for photoautotrophic growth and
RT contributes to antenna function.";
RL J. Biol. Chem. 275:31211-31218(2000).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Participates in efficiency of electron transfer from
CC plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria).
CC This plastocyanin-docking protein contributes to the specific
CC association of plastocyanin to PSI. {ECO:0000269|PubMed:10900198}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18431481}; Single-pass membrane protein; Lumenal
CC side.
CC -!- MISCELLANEOUS: Plants lacking PSAF have altered thylakoid structure and
CC are not viable.
CC -!- SIMILARITY: Belongs to the PsaF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ245629; CAB52747.1; -; mRNA.
DR EMBL; AC007654; AAF24595.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31342.1; -; Genomic_DNA.
DR EMBL; AF360251; AAK25961.1; -; mRNA.
DR EMBL; AY048220; AAK82483.1; -; mRNA.
DR EMBL; AY051021; AAK93698.1; -; mRNA.
DR EMBL; AF424564; AAL11558.1; -; mRNA.
DR EMBL; AY062712; AAL32790.1; -; mRNA.
DR EMBL; AY094015; AAM16171.1; -; mRNA.
DR EMBL; BT000680; AAN31826.1; -; mRNA.
DR EMBL; BT002099; AAN72110.1; -; mRNA.
DR EMBL; AY052281; AAK96474.1; -; mRNA.
DR EMBL; AY088009; AAM65555.1; -; mRNA.
DR RefSeq; NP_174418.1; NM_102871.5.
DR PDB; 7WFD; EM; 3.25 A; AF=1-221.
DR PDB; 7WFE; EM; 3.25 A; BF=1-221.
DR PDB; 7WG5; EM; 3.89 A; AF/BF=1-221.
DR PDBsum; 7WFD; -.
DR PDBsum; 7WFE; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; Q9SHE8; -.
DR SMR; Q9SHE8; -.
DR BioGRID; 25256; 32.
DR STRING; 3702.AT1G31330.1; -.
DR TCDB; 5.B.4.1.1; the plant photosystem i supercomplex (psi) family.
DR iPTMnet; Q9SHE8; -.
DR PaxDb; Q9SHE8; -.
DR PRIDE; Q9SHE8; -.
DR ProteomicsDB; 226321; -.
DR EnsemblPlants; AT1G31330.1; AT1G31330.1; AT1G31330.
DR GeneID; 840021; -.
DR Gramene; AT1G31330.1; AT1G31330.1; AT1G31330.
DR KEGG; ath:AT1G31330; -.
DR Araport; AT1G31330; -.
DR TAIR; locus:2197515; AT1G31330.
DR eggNOG; ENOG502QTZ8; Eukaryota.
DR HOGENOM; CLU_098828_0_1_1; -.
DR InParanoid; Q9SHE8; -.
DR OMA; TPCSESK; -.
DR OrthoDB; 1476188at2759; -.
DR PhylomeDB; Q9SHE8; -.
DR BioCyc; MetaCyc:MON-1092; -.
DR PRO; PR:Q9SHE8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHE8; baseline and differential.
DR Genevisible; Q9SHE8; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009538; C:photosystem I reaction center; IEA:InterPro.
DR GO; GO:0010287; C:plastoglobule; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.8.110; -; 1.
DR InterPro; IPR003666; PSI_PsaF.
DR InterPro; IPR036577; PSI_PsaF_sf.
DR PANTHER; PTHR34939; PTHR34939; 1.
DR Pfam; PF02507; PSI_PsaF; 1.
DR SUPFAM; SSF81536; SSF81536; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Photosynthesis; Photosystem I;
KW Plastid; Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 33..67
FT /note="Thylakoid"
FT /evidence="ECO:0000305"
FT CHAIN 68..221
FT /note="Photosystem I reaction center subunit III,
FT chloroplastic"
FT /id="PRO_0000342091"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 7
FT /note="A -> T (in Ref. 1; CAB52747 and 5; AAM65555)"
FT /evidence="ECO:0000305"
FT CONFLICT 31
FT /note="V -> F (in Ref. 4; AAK96474)"
FT /evidence="ECO:0000305"
FT CONFLICT 41
FT /note="T -> A (in Ref. 1; CAB52747 and 5; AAM65555)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24173 MW; 7793D4BE8F3CA38C CRC64;
MSLTIPANLV LNPRSNKSLT QSVPKSSARF VCSDDKSSSS TPQSMKAFSA AVALSSILLS
APMPAVADIS GLTPCKDSKQ FAKREKQQIK KLESSLKLYA PESAPALALN AQIEKTKRRF
DNYGKYGLLC GSDGLPHLIV NGDQRHWGEF ITPGILFLYI AGWIGWVGRS YLIAISGEKK
PAMKEIIIDV PLASRIIFRG FIWPVAAYRE FLNGDLIAKD V
