PROY_ECOLI
ID PROY_ECOLI Reviewed; 457 AA.
AC P0AAE2; P77327; Q2MC24;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Proline-specific permease ProY;
GN Name=proY; Synonyms=yajM; OrderedLocusNames=b0402, JW5055;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Permease that is involved in the transport across the
CC cytoplasmic membrane of proline.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family.
CC {ECO:0000305}.
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DR EMBL; U82664; AAB40158.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73505.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76182.1; -; Genomic_DNA.
DR PIR; B64769; B64769.
DR RefSeq; NP_414936.1; NC_000913.3.
DR RefSeq; WP_001295329.1; NZ_SSZK01000009.1.
DR AlphaFoldDB; P0AAE2; -.
DR SMR; P0AAE2; -.
DR BioGRID; 4262813; 4.
DR DIP; DIP-47977N; -.
DR IntAct; P0AAE2; 1.
DR STRING; 511145.b0402; -.
DR TCDB; 2.A.3.1.23; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; P0AAE2; -.
DR PRIDE; P0AAE2; -.
DR EnsemblBacteria; AAC73505; AAC73505; b0402.
DR EnsemblBacteria; BAE76182; BAE76182; BAE76182.
DR GeneID; 66671299; -.
DR GeneID; 945050; -.
DR KEGG; ecj:JW5055; -.
DR KEGG; eco:b0402; -.
DR PATRIC; fig|1411691.4.peg.1875; -.
DR EchoBASE; EB3376; -.
DR eggNOG; COG1113; Bacteria.
DR HOGENOM; CLU_007946_9_3_6; -.
DR InParanoid; P0AAE2; -.
DR OMA; PHWVWVL; -.
DR PhylomeDB; P0AAE2; -.
DR BioCyc; EcoCyc:PROY-MON; -.
DR PRO; PR:P0AAE2; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR004840; Amoino_acid_permease_CS.
DR Pfam; PF00324; AA_permease; 1.
DR PROSITE; PS00218; AMINO_ACID_PERMEASE_1; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..457
FT /note="Proline-specific permease ProY"
FT /id="PRO_0000054207"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..113
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..156
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..197
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..277
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..355
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..399
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 421..424
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 457 AA; 50216 MW; 5F2819526EA06BD8 CRC64;
MESKNKLKRG LSTRHIRFMA LGSAIGTGLF YGSADAIKMA GPSVLLAYII GGIAAYIIMR
ALGEMSVHNP AASSFSRYAQ ENLGPLAGYI TGWTYCFEIL IVAIADVTAF GIYMGVWFPT
VPHWIWVLSV VLIICAVNLM SVKVFGELEF WFSFFKVATI IIMIVAGFGI IIWGIGNGGQ
PTGIHNLWSN GGFFSNGWLG MVMSLQMVMF AYGGIEIIGI TAGEAKDPEK SIPRAINSVP
MRILVFYVGT LFVIMSIYPW NQVGTAGSPF VLTFQHMGIT FAASILNFVV LTASLSAINS
DVFGVGRMLH GMAEQGSAPK IFSKTSRRGI PWVTVLVMTT ALLFAVYLNY IMPENVFLVI
ASLATFATVW VWIMILLSQI AFRRRLPPEE VKALKFKVPG GVATTIGGLI FLLFIIGLIG
YHPDTRISLY VGFAWIVVLL IGWMFKRRHD RQLAENQ
