ID   PROT_GILLR              Reviewed;         565 AA.
AC   H2BXL5;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=Probable protease Gilli_2517;
DE            EC=3.4.-.- {ECO:0000305|PubMed:35025633};
GN   ORFNames=Gilli_2517 {ECO:0000312|EMBL:EHQ03139.1};
OS   Gillisia limnaea (strain DSM 15749 / LMG 21470 / R-8282).
OC   Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Gillisia.
OX   NCBI_TaxID=865937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15749 / LMG 21470 / R-8282;
RX   PubMed=23450183; DOI=10.4056/sigs.3216895;
RA   Riedel T., Held B., Nolan M., Lucas S., Lapidus A., Tice H., Del Rio T.G.,
RA   Cheng J.F., Han C., Tapia R., Goodwin L.A., Pitluck S., Liolios K.,
RA   Mavromatis K., Pagani I., Ivanova N., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Land M., Rohde M., Tindall B.J., Detter J.C., Goker M.,
RA   Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C.,
RA   Klenk H.P., Woyke T.;
RT   "Genome sequence of the Antarctic rhodopsins-containing flavobacterium
RT   Gillisia limnaea type strain (R-8282(T)).";
RL   Stand. Genomic Sci. 7:107-119(2012).
RN   [2]
RP   FUNCTION.
RC   STRAIN=DSM 15749 / LMG 21470 / R-8282;
RX   PubMed=35025633; DOI=10.1126/science.abj8432;
RA   Johnson A.G., Wein T., Mayer M.L., Duncan-Lowey B., Yirmiya E.,
RA   Oppenheimer-Shaanan Y., Amitai G., Sorek R., Kranzusch P.J.;
RT   "Bacterial gasdermins reveal an ancient mechanism of cell death.";
RL   Science 375:221-225(2022).
CC   -!- FUNCTION: Probably a dedicated protease for substrate gasdermin bGSDM;
CC       cleaves the bGSDM precursor, releasing the pore-forming moiety, which
CC       integrates into the membrane and triggers cell death. Involved in
CC       defense against bacteriophages (Probable). Expression of bGSDM and this
CC       neighboring protease is not toxic in E.coli (PubMed:35025633).
CC       {ECO:0000269|PubMed:35025633, ECO:0000305|PubMed:35025633}.
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DR   EMBL; JH594606; EHQ03139.1; -; Genomic_DNA.
DR   RefSeq; WP_006989446.1; NZ_JH594606.1.
DR   STRING; 865937.Gilli_2517; -.
DR   EnsemblBacteria; EHQ03139; EHQ03139; Gilli_2517.
DR   eggNOG; COG1672; Bacteria.
DR   HOGENOM; CLU_482140_0_0_10; -.
DR   OrthoDB; 1171844at2; -.
DR   Proteomes; UP000003844; Unassembled WGS sequence.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Antiviral defense; Hydrolase; Protease; Reference proteome.
FT   CHAIN           1..565
FT                   /note="Probable protease Gilli_2517"
FT                   /id="PRO_0000455578"
SQ   SEQUENCE   565 AA;  65684 MW;  1C88AD33618CDC21 CRC64;
     MHIIFQYPIV DLRDIVSGGN GRLNDPKWPD PQERRQSFVS GFGKVKSRNL GGSDNFTGES
     YYCDSHSAIK FKELQHQGFS EGITTPASIF NSYRRYYNDG RFVGKVEIGL IDNLEKIIRN
     YPGSEGIQIS SILKHYSNLE ATVEDEQVKL YKAGPRLSKK YQRESTLREK HFQINPDYVQ
     TGELTIVLTY SSHERLIVPR RSFSLEKIEL PNDAGSIELF GYKLKQDGYP TKVWIIKIPA
     NFRSKSGKHK TILRDLRMNL LRIHLEKETI KILLNAIKYK QIELEKESAE AKQVNAYFEQ
     TSKKLFRKSR YDIKQENLLD FALQSEKSMD KGSFNSLKEN ITYFQDEFML DNLGKLVGSM
     AVKPMLFVCS NPRDSNFIDF DKEYKDLKYN LQRAIDRDHY DIEIELSVTK DEFKDILDRY
     KPQFLHLSMH ATVKDGLHFE DKNKAILPMS VKEFKQIIER YTKKHELKLV LISACNSKNH
     AKAIKEYCDF AIGTKAVFPV PAALIYSNNF YTTLFNGYQK DLEYCHSGAI NAIEFNNPKF
     DDLDYKNKKI RVHEIPVLIK NSKYV