ID   PROTO_ORADR             Reviewed;          71 AA.
AC   P86147; C7DT08;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Venom peptide 2-long {ECO:0000305|PubMed:19857508};
DE            Short=OdVP2L {ECO:0000303|PubMed:19857508};
DE   Contains:
DE     RecName: Full=Orancis-protonectin {ECO:0000303|PubMed:18695936};
DE     AltName: Full=Venom peptide 2 {ECO:0000305|PubMed:19857508, ECO:0000312|EMBL:ACU30739.1};
DE              Short=OdVP2 {ECO:0000303|PubMed:19857508};
DE   Flags: Precursor;
GN   Name=VP2;
OS   Orancistrocerus drewseni (Solitary wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Eumeninae; Orancistrocerus.
OX   NCBI_TaxID=529024;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 55-68, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND AMIDATION AT LEU-68.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=19857508; DOI=10.1016/j.toxicon.2009.10.023;
RA   Baek J.H., Lee S.H.;
RT   "Isolation and molecular cloning of venom peptides from Orancistrocerus
RT   drewseni (Hymenoptera: Eumenidae).";
RL   Toxicon 55:711-718(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 57-68, FUNCTION, SUBCELLULAR LOCATION, MASS
RP   SPECTROMETRY, AND AMIDATION AT LEU-68.
RC   TISSUE=Venom;
RX   PubMed=18695936; DOI=10.1007/s00726-008-0166-y;
RA   Murata K., Shinada T., Ohfune Y., Hisada M., Yasuda A., Naoki H.,
RA   Nakajima T.;
RT   "Novel mastoparan and protonectin analogs isolated from a solitary wasp,
RT   Orancistrocerus drewseni drewseni.";
RL   Amino Acids 37:389-394(2009).
RN   [3]
RP   REVIEW.
RX   PubMed=27096870; DOI=10.3390/toxins8040114;
RA   Konno K., Kazuma K., Nihei K.;
RT   "Peptide toxins in solitary wasp venoms.";
RL   Toxins 8:114-114(2016).
CC   -!- FUNCTION: Has potent hemolytic activity against ovine erythrocytes at
CC       micromolar concentrations. Also exhibits strong antimicrobial activity
CC       against the fungi B.cinerea (MIC=0.5 ug/ml) and C.albicans, and
CC       moderate activity against the Gram-positive bacterium S.aureus (MIC=25
CC       ug/ml) and the Gram-negative bacterium E.coli. Venom peptide 2-long has
CC       substantially lower antimicrobial activity against C.albicans (MIC=100
CC       ug/ml) and bacteria than Orancis-protonectin but has equivalent
CC       activity against B.cinerea (MIC=0.5 ug/ml).
CC       {ECO:0000269|PubMed:18695936, ECO:0000269|PubMed:19857508}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18695936,
CC       ECO:0000269|PubMed:19857508}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:18695936, ECO:0000305|PubMed:19857508}.
CC   -!- MASS SPECTROMETRY: [Orancis-protonectin]: Mass=1269.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18695936};
CC   -!- MASS SPECTROMETRY: [Orancis-protonectin]: Mass=1268.8;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:18695936};
CC   -!- MASS SPECTROMETRY: [Venom peptide 2-long]: Mass=1494.96; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19857508};
CC   -!- MASS SPECTROMETRY: [Orancis-protonectin]: Mass=1268.86; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19857508};
CC   -!- SIMILARITY: Belongs to the MCD family. Protonectin subfamily.
CC       {ECO:0000255}.
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DR   EMBL; GQ205581; ACU30739.1; -; mRNA.
DR   AlphaFoldDB; P86147; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Fungicide; Hemolysis; Secreted; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..54
FT                   /evidence="ECO:0000269|PubMed:19857508"
FT                   /id="PRO_0000390691"
FT   PEPTIDE         55..68
FT                   /note="Venom peptide 2-long"
FT                   /evidence="ECO:0000269|PubMed:19857508"
FT                   /id="PRO_0000390692"
FT   PEPTIDE         57..68
FT                   /note="Orancis-protonectin"
FT                   /evidence="ECO:0000269|PubMed:18695936"
FT                   /id="PRO_0000372684"
FT   MOD_RES         68
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:18695936,
FT                   ECO:0000269|PubMed:19857508"
SQ   SEQUENCE   71 AA;  7172 MW;  58099ACCDD5B03F9 CRC64;
     MKQSIIIALF ATIAVMACLQ MVAAVPAPVP EAAPGPVAEA EAYASPEALA SPEAEPILGI
     ITSLLKSLGK K