ID   PROT6_EUMPO             Reviewed;          62 AA.
AC   D1MEJ3;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Venom peptide 6 {ECO:0000303|PubMed:20096300};
DE            Short=EpVP6 {ECO:0000303|PubMed:20096300};
DE            Short=VP6 {ECO:0000312|EMBL:ACZ37399.1};
DE   AltName: Full=Protonectin VP6 {ECO:0000305};
DE   Flags: Precursor;
OS   Eumenes pomiformis (Potter wasp) (Vespa pomiformis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Eumeninae; Eumenes.
OX   NCBI_TaxID=693051;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=20096300; DOI=10.1016/j.toxicon.2010.01.004;
RA   Baek J.H., Lee S.H.;
RT   "Differential gene expression profiles in the venom gland/sac of Eumenes
RT   pomiformis (Hymenoptera: Eumenidae).";
RL   Toxicon 55:1147-1156(2010).
CC   -!- FUNCTION: Potent antimicrobial peptide against both Gram-positive and
CC       Gram-negative bacteria. May act by disrupting the integrity of the
CC       outer and inner bacterial membranes. May adopt an amphipathic alpha
CC       helical conformation in membrane that is essential for the membrane
CC       disrupting activity. Also acts as a mast cell degranulator that induces
CC       a potent chemotaxis in polymorphonucleated leukocyte (PMNL) cells.
CC       Shows no or weak hemolytic activity. {ECO:0000250|UniProtKB:P69437}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:20096300}. Target
CC       cell membrane {ECO:0000305}. Note=Assumes an amphipathic alpha-helical
CC       conformation in a lipid environment. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:20096300}.
CC   -!- MISCELLANEOUS: Not found in venom, suggesting that it is a minor
CC       component. {ECO:0000305|PubMed:20096300}.
CC   -!- SIMILARITY: Belongs to the MCD family. Protonectin subfamily.
CC       {ECO:0000305}.
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DR   EMBL; GU136238; ACZ37399.1; -; mRNA.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Membrane; Secreted; Signal; Target cell membrane; Target membrane.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..46
FT                   /evidence="ECO:0000305|PubMed:20096300"
FT                   /id="PRO_0000453662"
FT   PEPTIDE         47..62
FT                   /note="Venom peptide 6"
FT                   /evidence="ECO:0000305|PubMed:20096300"
FT                   /id="PRO_5003024362"
SQ   SEQUENCE   62 AA;  6286 MW;  FC11DFD382E76A8F CRC64;
     MKSTSVFILF AGIAIMACLQ MTGTEAAPSA SPNPTPVARA DPDPEAFGPV IGLLSGILKS
     LL