PROT3_ARATH
ID PROT3_ARATH Reviewed; 436 AA.
AC Q9SJP9; Q8LDD5;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Proline transporter 3;
DE Short=AtPROT3;
GN Name=PROT3; OrderedLocusNames=At2g36590; ORFNames=F1O11.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15618414; DOI=10.1104/pp.104.055079;
RA Grallath S., Weimar T., Meyer A., Gumy C., Suter-Grotemeyer M.,
RA Neuhaus J.M., Rentsch D.;
RT "The AtProT family. Compatible solute transporters with similar substrate
RT specificity but differential expression patterns.";
RL Plant Physiol. 137:117-126(2005).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=20959625; DOI=10.1093/jxb/erq320;
RA Lehmann S., Gumy C., Blatter E., Boeffel S., Fricke W., Rentsch D.;
RT "In planta function of compatible solute transporters of the AtProT
RT family.";
RL J. Exp. Bot. 62:787-796(2011).
CC -!- FUNCTION: Proline transporter that mediates proline and glycine betaine
CC transport. When expressed in a heterologous system (yeast), imports L-
CC proline, glycine betaine and GABA across the plasma membrane.
CC {ECO:0000269|PubMed:15618414}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.29 mM for glycine betaine {ECO:0000269|PubMed:15618414};
CC KM=0.999 mM for L-proline {ECO:0000269|PubMed:15618414};
CC KM=5.12 mM for GABA {ECO:0000269|PubMed:15618414};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:15618414};
CC Multi-pass membrane protein {ECO:0000305|PubMed:15618414}. Note=Plasma
CC membrane.
CC -!- TISSUE SPECIFICITY: Expressed in epidermal cells of leaves, sepals and
CC petals. {ECO:0000269|PubMed:15618414}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:20959625}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.3) subfamily.
CC {ECO:0000305}.
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DR EMBL; AC006919; AAD24641.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09272.1; -; Genomic_DNA.
DR EMBL; BT004018; AAO42054.1; -; mRNA.
DR EMBL; AY086068; AAM63275.1; -; mRNA.
DR PIR; D84782; D84782.
DR RefSeq; NP_181198.1; NM_129215.4.
DR AlphaFoldDB; Q9SJP9; -.
DR SMR; Q9SJP9; -.
DR BioGRID; 3576; 1.
DR STRING; 3702.AT2G36590.1; -.
DR PaxDb; Q9SJP9; -.
DR PRIDE; Q9SJP9; -.
DR ProteomicsDB; 226376; -.
DR EnsemblPlants; AT2G36590.1; AT2G36590.1; AT2G36590.
DR GeneID; 818232; -.
DR Gramene; AT2G36590.1; AT2G36590.1; AT2G36590.
DR KEGG; ath:AT2G36590; -.
DR Araport; AT2G36590; -.
DR TAIR; locus:2044835; AT2G36590.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_3_0_1; -.
DR InParanoid; Q9SJP9; -.
DR OMA; MSFRIVV; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q9SJP9; -.
DR SABIO-RK; Q9SJP9; -.
DR PRO; PR:Q9SJP9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJP9; baseline and differential.
DR Genevisible; Q9SJP9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015824; P:proline transport; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..436
FT /note="Proline transporter 3"
FT /id="PRO_0000418995"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 13
FT /note="G -> D (in Ref. 4; AAM63275)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="L -> F (in Ref. 4; AAM63275)"
FT /evidence="ECO:0000305"
FT CONFLICT 394
FT /note="N -> S (in Ref. 4; AAM63275)"
FT /evidence="ECO:0000305"
FT CONFLICT 400
FT /note="C -> W (in Ref. 4; AAM63275)"
FT /evidence="ECO:0000305"
FT CONFLICT 425
FT /note="L -> I (in Ref. 4; AAM63275)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 436 AA; 47651 MW; DE2786FDE5F10747 CRC64;
MNSKNRINNV GEGVDIEIPD TAHQISSDSW FQAAFVLTTS INSAYVLGYS GTVMVPLGWI
GGVVGLILAT AISLYANTLV AKLHEFGGKR HIRYRDLAGF IYGRKAYCLT WVLQYVNLFM
INCGFIILAG SALKAVYVLF RDDHAMKLPH FIAIAGLICA VFAIGIPHLS ALGIWLAVST
ILSLIYIVVA IVLSVKDGVK APSRDYEIQG SPLSKLFTIT GAAATLVFVF NTGMLPEIQA
TVKQPVVKNM MKALYFQFTV GVLPMFAVVF IGYWAYGSST SPYLLNNVNG PLWVKALANI
SAILQSVISL HIFASPTYEY MDTKFGIKGN PLALKNLLFR IMARGGYIAV STLLSALLPF
LGDFMSLTGA VSTFPLTFIL ANHMYYKAKN NKLNTLQKLC HWLNVVFFSL MSVAAAIAAL
RLIALDSKNF HVFADL
