ID   PROT2_LYSEN             Reviewed;         641 AA.
AC   A0A0S2DN74;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Probable serine protease FE772_23065 {ECO:0000305};
DE            EC=3.4.21.- {ECO:0000305|PubMed:31540995};
DE   AltName: Full=Trypsin-like protease 2 {ECO:0000303|PubMed:35025633};
GN   ORFNames=FE772_23065 {ECO:0000312|EMBL:QCW28097.1},
GN   Ga0399710_4915 {ECO:0000303|PubMed:35025633},
GN   GLE_4745 {ECO:0000312|EMBL:ALN60086.1};
OS   Lysobacter enzymogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Lysobacter.
OX   NCBI_TaxID=69;
RN   [1] {ECO:0000312|EMBL:ALN60086.1, ECO:0000312|Proteomes:UP000061569}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C3;
RX   PubMed=26597042; DOI=10.1186/s12864-015-2191-z;
RA   de Bruijn I., Cheng X., de Jager V., Exposito R.G., Watrous J., Patel N.,
RA   Postma J., Dorrestein P.C., Kobayashi D., Raaijmakers J.M.;
RT   "Comparative genomics and metabolic profiling of the genus Lysobacter.";
RL   BMC Genomics 16:991-991(2015).
RN   [2] {ECO:0000312|EMBL:QCW28097.1, ECO:0000312|Proteomes:UP000308311}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YC36;
RX   PubMed=31540995; DOI=10.1128/aem.01742-19;
RA   Feng T., Han Y., Li B., Li Z., Yu Y., Sun Q., Li X., Du L., Zhang X.H.,
RA   Wang Y.;
RT   "Interspecies and Intraspecies Signals Synergistically Regulate Lysobacter
RT   enzymogenes Twitching Motility.";
RL   Appl. Environ. Microbiol. 85:0-0(2019).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF SER-296.
RC   STRAIN=YC36;
RX   PubMed=35025633; DOI=10.1126/science.abj8432;
RA   Johnson A.G., Wein T., Mayer M.L., Duncan-Lowey B., Yirmiya E.,
RA   Oppenheimer-Shaanan Y., Amitai G., Sorek R., Kranzusch P.J.;
RT   "Bacterial gasdermins reveal an ancient mechanism of cell death.";
RL   Science 375:221-225(2022).
CC   -!- FUNCTION: Possibly a dedicated protease for substrate gasdermin bGSDM;
CC       cleaves the bGSDM precursor, releasing the pore-forming moiety, which
CC       integrates into the membrane and triggers cell death. Involved in
CC       defense against bacteriophages. When this probable 4 gene operon
CC       (bGSDM-FE772_23060-FE772_23065-FE772_23070) is inserted into E.coli it
CC       provides nearly 100-fold protection against phages T5 and T6 and about
CC       8-fold against phage T4. The operon without bGSDM no longer protects
CC       against phage. {ECO:0000269|PubMed:35025633}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000305}.
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DR   EMBL; CP013140; ALN60086.1; -; Genomic_DNA.
DR   EMBL; CP040656; QCW28097.1; -; Genomic_DNA.
DR   RefSeq; WP_057949282.1; NZ_CP013140.1.
DR   STRING; 69.GLE_4745; -.
DR   EnsemblBacteria; ALN60086; ALN60086; GLE_4745.
DR   KEGG; lez:GLE_4745; -.
DR   PATRIC; fig|69.6.peg.4679; -.
DR   OrthoDB; 1468733at2; -.
DR   Proteomes; UP000061569; Chromosome.
DR   Proteomes; UP000308311; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Hydrolase; Membrane;
KW   Protease; Reference proteome; Serine protease; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..641
FT                   /note="Probable serine protease FE772_23065"
FT                   /id="PRO_0000455585"
FT   TRANSMEM        532..552
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         296
FT                   /note="S->A: 4-gene operon no longer protects against
FT                   phage."
FT                   /evidence="ECO:0000269|PubMed:35025633"
SQ   SEQUENCE   641 AA;  69571 MW;  559DE1AF8D083B97 CRC64;
     MVAVVQADYS RAEALAAWTR LSDEFIGNCY VSVRPRHAPA WEVVVASAAG SLRLEAFKRA
     HDHDFLDRLA VAIGNWEQKA QRPDHEIAQM LDQVGDYGLM QGMTNPDKGF MHADILLPLL
     QARDACAIVV RTEPVFQQLG TAFLVRPDLI LTAAHVVMDV DAATGRWAST LKNGLAFHFR
     EKPNQREHPT LAIRPAAVAL ISHALPHGRP PNLLERSLAA PADTCLDYAL IRLAQRVSHL
     RPVEVVDTAA VKQGKPCWAF GFPGGNALMM DVDLVTDIDP GSGRWLHRAN VAAGMSGGCC
     INHEGQVAGL HEGTLDSEDD AKVKRNRGIS IAAIRRDQCR DGKDPLKQVA SSPSLEFRDP
     ALVDGWYRAG TALAGEAGAA QWRASVEAAL RGRNPDATDS LPAYHPWFAR ADVEKWIDSA
     APDERLSLIH GPPGVGKSFC IHLLRGKLDP YADLVVFNPT QTNDMTWSDA TGHAAAANAS
     DYRTAAASVR YRAIDDFLGE LRDRSAGGTR TCYVAIDFGP AGRQDRFVGT NWVELIAILA
     AAGWIRVMLI GLDDYERSVM IDRMESRPET DSVKIAESEL APITAAEFRT YAKHLASARG
     KPAPKQAEMA KYVDSAVVGV AEPMKMVAAV RAAIELEAAL S