ID   PROT1_LYSEN             Reviewed;         387 AA.
AC   A0A0S2DNK1;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=Probable serine protease FE772_23060 {ECO:0000305};
DE            EC=3.4.21.- {ECO:0000305|PubMed:28473380};
DE   AltName: Full=Trypsin-like protease 1 {ECO:0000303|PubMed:35025633};
GN   ORFNames=BV903_13690 {ECO:0000312|EMBL:OPD63339.1},
GN   FE772_23060 {ECO:0000312|EMBL:QCW28096.1},
GN   Ga0399710_4914 {ECO:0000303|PubMed:35025633},
GN   GLE_4744 {ECO:0000312|EMBL:ALN60085.1};
OS   Lysobacter enzymogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Lysobacter.
OX   NCBI_TaxID=69;
RN   [1] {ECO:0000312|EMBL:ALN60085.1, ECO:0000312|Proteomes:UP000061569}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C3;
RX   PubMed=26597042; DOI=10.1186/s12864-015-2191-z;
RA   de Bruijn I., Cheng X., de Jager V., Exposito R.G., Watrous J., Patel N.,
RA   Postma J., Dorrestein P.C., Kobayashi D., Raaijmakers J.M.;
RT   "Comparative genomics and metabolic profiling of the genus Lysobacter.";
RL   BMC Genomics 16:991-991(2015).
RN   [2] {ECO:0000312|EMBL:OPD63339.1, ECO:0000312|Proteomes:UP000190203}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B25;
RX   PubMed=28473380; DOI=10.1128/genomea.00271-17;
RA   Hernandez I., Fernandez C.;
RT   "Draft Genome Sequence and Assembly of a Lysobacter enzymogenes Strain with
RT   Biological Control Activity against Root Knot Nematodes.";
RL   Genome Announc. 5:0-0(2017).
RN   [3] {ECO:0000312|EMBL:QCW28096.1, ECO:0000312|Proteomes:UP000308311}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YC36;
RX   PubMed=31540995; DOI=10.1128/aem.01742-19;
RA   Feng T., Han Y., Li B., Li Z., Yu Y., Sun Q., Li X., Du L., Zhang X.H.,
RA   Wang Y.;
RT   "Interspecies and Intraspecies Signals Synergistically Regulate Lysobacter
RT   enzymogenes Twitching Motility.";
RL   Appl. Environ. Microbiol. 85:0-0(2019).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF SER-343.
RC   STRAIN=YC36;
RX   PubMed=35025633; DOI=10.1126/science.abj8432;
RA   Johnson A.G., Wein T., Mayer M.L., Duncan-Lowey B., Yirmiya E.,
RA   Oppenheimer-Shaanan Y., Amitai G., Sorek R., Kranzusch P.J.;
RT   "Bacterial gasdermins reveal an ancient mechanism of cell death.";
RL   Science 375:221-225(2022).
CC   -!- FUNCTION: Possibly a dedicated protease for substrate gasdermin bGSDM;
CC       cleaves the bGSDM precursor, releasing the pore-forming moiety, which
CC       integrates into the membrane and triggers cell death. Involved in
CC       defense against bacteriophages. When this probable 4 gene operon
CC       (bGSDM-FE772_23060-FE772_23065-FE772_23070) is inserted into E.coli it
CC       provides nearly 100-fold protection against phages T5 and T6 and about
CC       8-fold against phage T4. The operon without bGSDM no longer protects
CC       against phage. {ECO:0000269|PubMed:35025633}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000305}.
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DR   EMBL; CP013140; ALN60085.1; -; Genomic_DNA.
DR   EMBL; MTAY01000035; OPD63339.1; -; Genomic_DNA.
DR   EMBL; CP040656; QCW28096.1; -; Genomic_DNA.
DR   RefSeq; WP_057949281.1; NZ_CP013140.1.
DR   EnsemblBacteria; ALN60085; ALN60085; GLE_4744.
DR   EnsemblBacteria; OPD63339; OPD63339; BV903_13690.
DR   KEGG; lez:GLE_4744; -.
DR   PATRIC; fig|69.6.peg.4678; -.
DR   OMA; VGYPAWD; -.
DR   OrthoDB; 390088at2; -.
DR   Proteomes; UP000061569; Chromosome.
DR   Proteomes; UP000190203; Unassembled WGS sequence.
DR   Proteomes; UP000308311; Chromosome.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.10.10; -; 1.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   SUPFAM; SSF50494; SSF50494; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Hydrolase; Protease; Reference proteome;
KW   Serine protease.
FT   CHAIN           1..387
FT                   /note="Probable serine protease FE772_23060"
FT                   /id="PRO_0000455584"
FT   MUTAGEN         343
FT                   /note="S->A: 4-gene operon still protects against phage."
FT                   /evidence="ECO:0000269|PubMed:35025633"
SQ   SEQUENCE   387 AA;  41018 MW;  02D4898C7116C6CB CRC64;
     MEQERIRQLA KLLAAGARAR DAQAESMMDG GVAAAPAPAG QTLDVVEQAL STPPDDVDET
     QWRAAREQLL THAHNGLVKL QRGDLQLDAD EGCAMEAVII SDGSRPSFLL CDGEIDPKDP
     SIETWAGNIA AAQALGIAKL AAAVGRIQPK NGHASRYVGT GTLIDRDAGL ILTNYHVIEQ
     AQQNYGVAMT RNGDRLSVDG WLEIDFVGES CSLRTHRFRI VEVALPQGYG STFHGIDAAV
     ARIEPLPDSP ALPDPVPLLS ADAAYATGAI SSLALIGFPA RPSLQDGKDV DWSFVMRVLF
     GNRFGVKRLA PGQFTLPLGS HALDQGRRAI GHDATTFGGA SGSLLMSWLD DRTPSFALHF
     GGATGVSNYA LSFAAERNAL SAIGARF