PROT1_ARATH
ID PROT1_ARATH Reviewed; 442 AA.
AC P92961; O04746; Q8LAE3;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Proline transporter 1;
DE Short=AtPROT1;
GN Name=PROT1; OrderedLocusNames=At2g39890; ORFNames=T28M21.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Leaf;
RX PubMed=8776904; DOI=10.2307/3870312;
RA Rentsch D., Hirner B., Schmelzer E., Frommer W.B.;
RT "Salt stress-induced proline transporters and salt stress-repressed broad
RT specificity amino acid permeases identified by suppression of a yeast amino
RT acid permease-targeting mutant.";
RL Plant Cell 8:1437-1446(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=10359089; DOI=10.1016/s0014-5793(99)00516-5;
RA Breitkreuz K.E., Shelp B.J., Fischer W.-N., Schwacke R., Rentsch D.;
RT "Identification and characterization of GABA, proline and quaternary
RT ammonium compound transporters from Arabidopsis thaliana.";
RL FEBS Lett. 450:280-284(1999).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=15618414; DOI=10.1104/pp.104.055079;
RA Grallath S., Weimar T., Meyer A., Gumy C., Suter-Grotemeyer M.,
RA Neuhaus J.M., Rentsch D.;
RT "The AtProT family. Compatible solute transporters with similar substrate
RT specificity but differential expression patterns.";
RL Plant Physiol. 137:117-126(2005).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20959625; DOI=10.1093/jxb/erq320;
RA Lehmann S., Gumy C., Blatter E., Boeffel S., Fricke W., Rentsch D.;
RT "In planta function of compatible solute transporters of the AtProT
RT family.";
RL J. Exp. Bot. 62:787-796(2011).
CC -!- FUNCTION: Proline transporter that mediates proline and glycine betaine
CC transport. May be involved in long-distance transport of proline and
CC required for phloem loading, retrieval of proline leaking from the
CC phloem, or in xylem-to-phloem transfer. When expressed in a
CC heterologous system (yeast), imports D- and L-proline, glycine betaine
CC and GABA across the plasma membrane. Has the same affinity for D- and
CC L-proline. {ECO:0000269|PubMed:10359089, ECO:0000269|PubMed:15618414,
CC ECO:0000269|PubMed:20959625, ECO:0000269|PubMed:8776904}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.115 mM for glycine betaine {ECO:0000269|PubMed:15618414};
CC KM=0.427 mM for L-proline {ECO:0000269|PubMed:15618414};
CC KM=4.5 mM for GABA {ECO:0000269|PubMed:15618414};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:15618414};
CC Multi-pass membrane protein {ECO:0000305|PubMed:15618414}. Note=Plasma
CC membrane.
CC -!- TISSUE SPECIFICITY: Expressed in vascular tissues of roots, leaves,
CC stems, sepals, petals, stamen and siliques. Expressed in pollen.
CC {ECO:0000269|PubMed:15618414, ECO:0000269|PubMed:20959625,
CC ECO:0000269|PubMed:8776904}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:20959625}.
CC -!- MISCELLANEOUS: Treatment with toxic concentrations of proline reduces
CC shoot growth and root elongation in wild-type plants, while plant lines
CC over-expressing PROT1 stop growing shortly after unfolding of
CC cotyledons. {ECO:0000305|PubMed:20959625}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.3) subfamily.
CC {ECO:0000305}.
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DR EMBL; X95737; CAA65052.1; -; mRNA.
DR EMBL; AF002109; AAB95274.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09745.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09746.1; -; Genomic_DNA.
DR EMBL; AY087868; AAM65420.1; -; mRNA.
DR PIR; T50692; T50692.
DR RefSeq; NP_001078025.1; NM_001084556.2.
DR RefSeq; NP_181518.1; NM_129547.3.
DR AlphaFoldDB; P92961; -.
DR SMR; P92961; -.
DR BioGRID; 3914; 16.
DR IntAct; P92961; 16.
DR STRING; 3702.AT2G39890.1; -.
DR TCDB; 2.A.18.3.1; the amino acid/auxin permease (aaap) family.
DR PaxDb; P92961; -.
DR PRIDE; P92961; -.
DR ProteomicsDB; 226375; -.
DR EnsemblPlants; AT2G39890.1; AT2G39890.1; AT2G39890.
DR EnsemblPlants; AT2G39890.2; AT2G39890.2; AT2G39890.
DR GeneID; 818576; -.
DR Gramene; AT2G39890.1; AT2G39890.1; AT2G39890.
DR Gramene; AT2G39890.2; AT2G39890.2; AT2G39890.
DR KEGG; ath:AT2G39890; -.
DR Araport; AT2G39890; -.
DR TAIR; locus:2061156; AT2G39890.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_3_0_1; -.
DR InParanoid; P92961; -.
DR OMA; SITWALQ; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; P92961; -.
DR SABIO-RK; P92961; -.
DR PRO; PR:P92961; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P92961; baseline and differential.
DR Genevisible; P92961; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015824; P:proline transport; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..442
FT /note="Proline transporter 1"
FT /id="PRO_0000418993"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 82
FT /note="A -> T (in Ref. 4; AAM65420)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 442 AA; 48456 MW; C630B01CE9A54A2C CRC64;
MTATEAKNRK INVGDGDDVV DIEIPDTAHQ ISSDSWFQVA FVLTTGINSA YVLGYSGTIM
VPLGWIGGVV GLLIATAISL YANTLIAKLH EFGGRRHIRY RDLAGFIYGR KAYHLTWGLQ
YVNLFMINCG FIILAGSALK AVYVLFRDDH TMKLPHFIAI AGLICAIFAI GIPHLSALGV
WLGVSTFLSL IYIVVAIVLS VRDGVKTPSR DYEIQGSSLS KLFTITGAAA NLVFAFNTGM
LPEIQATVRQ PVVKNMMKAL YFQFTAGVLP MYAVTFIGYW AYGSSTSTYL LNSVNGPLWV
KALANVSAIL QSVISLHIFA SPTYEYMDTK YGIKGNPFAI KNLLFRIMAR GGYIAVSTLI
SALLPFLGDF MSLTGAVSTF PLTFILANHM YYKAKNNKLN AMQKLWHWLN VVFFSLMSVA
AAIAAVRLIA VDSKNFHVFA DL
