PROS_DROME
ID PROS_DROME Reviewed; 1703 AA.
AC P29617; A0A0B4K660; Q6AWI5; Q95SP0; Q9U6A2; Q9VGP8;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 3.
DT 03-AUG-2022, entry version 209.
DE RecName: Full=Homeobox protein prospero;
GN Name=pros; ORFNames=CG17228;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M), FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=1720353; DOI=10.1016/0092-8674(91)90367-8;
RA Vaessin H., Grell E., Wolff E., Bier E., Jan L.Y., Jan Y.N.;
RT "Prospero is expressed in neuronal precursors and encodes a nuclear protein
RT that is involved in the control of axonal outgrowth in Drosophila.";
RL Cell 67:941-953(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 201-1703 (ISOFORM M), FUNCTION, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo;
RX PubMed=1842358;
RA Chu-Lagraff Q., Wright D.M., McNeil L.K., Doe C.Q.;
RT "The prospero gene encodes a divergent homeodomain protein that controls
RT neuronal identity in Drosophila.";
RL Development Suppl. 2:79-85(1991).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 201-1703 (ISOFORM M), FUNCTION, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S;
RX PubMed=1540176; DOI=10.1016/0006-291x(92)91878-t;
RA Matsuzaki F., Koizumi K., Hama C., Yoshioka T., Nabeshima Y.;
RT "Cloning of the Drosophila prospero gene and its expression in ganglion
RT mother cells.";
RL Biochem. Biophys. Res. Commun. 182:1326-1332(1992).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 205-1703 (ISOFORM H).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 301-1703, AND FUNCTION.
RX PubMed=11051550; DOI=10.1016/s0092-8674(00)00107-0;
RA Xu C., Kauffmann R.C., Zhang J., Kladny S., Carthew R.W.;
RT "Overlapping activators and repressors delimit transcriptional response to
RT receptor tyrosine kinase signals in the Drosophila eye.";
RL Cell 103:87-97(2000).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1061-1703 (ISOFORM I).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [9]
RP SIMILARITY TO C.ELEGANS CEH-26.
RX PubMed=7909177; DOI=10.1016/0968-0004(94)90035-3;
RA Buerglin T.R.;
RT "A Caenorhabditis elegans prospero homologue defines a novel domain.";
RL Trends Biochem. Sci. 19:70-71(1994).
RN [10]
RP FUNCTION.
RX PubMed=9380747; DOI=10.1073/pnas.94.20.10991;
RA Hassan B., Li L., Bremer K.A., Chang W., Pinsonneault J., Vaessin H.;
RT "Prospero is a panneural transcription factor that modulates homeodomain
RT protein activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:10991-10996(1997).
RN [11]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16564014; DOI=10.1016/j.cell.2006.01.038;
RA Betschinger J., Mechtler K., Knoblich J.A.;
RT "Asymmetric segregation of the tumor suppressor brat regulates self-renewal
RT in Drosophila neural stem cells.";
RL Cell 124:1241-1253(2006).
RN [12]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18342578; DOI=10.1016/j.devcel.2008.03.004;
RA Bowman S.K., Rolland V., Betschinger J., Kinsey K.A., Emery G.,
RA Knoblich J.A.;
RT "The tumor suppressors Brat and Numb regulate transit-amplifying neuroblast
RT lineages in Drosophila.";
RL Dev. Cell 14:535-546(2008).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-479; SER-482; SER-485;
RP SER-497; SER-651 AND SER-654, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [14]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=20152183; DOI=10.1016/j.devcel.2009.12.007;
RA Weng M., Golden K.L., Lee C.Y.;
RT "dFezf/Earmuff maintains the restricted developmental potential of
RT intermediate neural progenitors in Drosophila.";
RL Dev. Cell 18:126-135(2010).
RN [15]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23056424; DOI=10.1371/journal.pone.0046724;
RA Zhu S., Wildonger J., Barshow S., Younger S., Huang Y., Lee T.;
RT "The bHLH repressor Deadpan regulates the self-renewal and specification of
RT Drosophila larval neural stem cells independently of Notch.";
RL PLoS ONE 7:E46724-E46724(2012).
RN [16]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=27510969; DOI=10.1242/dev.137281;
RA Xie Y., Li X., Deng X., Hou Y., O'Hara K., Urso A., Peng Y., Chen L.,
RA Zhu S.;
RT "The Ets protein Pointed prevents both premature differentiation and
RT dedifferentiation of Drosophila intermediate neural progenitors.";
RL Development 143:3109-3118(2016).
RN [17]
RP X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 1545-1696, FUNCTION, AND DOMAIN.
RX PubMed=12429095; DOI=10.1016/s0969-2126(02)00883-3;
RA Ryter J.M., Doe C.Q., Matthews B.W.;
RT "Structure of the DNA binding region of prospero reveals a novel homeo-
RT prospero domain.";
RL Structure 10:1541-1549(2002).
RN [18]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 1541-1703, FUNCTION, AND DOMAIN.
RX PubMed=15837198; DOI=10.1016/j.str.2005.01.023;
RA Yousef M.S., Matthews B.W.;
RT "Structural basis of Prospero-DNA interaction: implications for
RT transcription regulation in developing cells.";
RL Structure 13:601-607(2005).
CC -!- FUNCTION: Homeodomain protein that controls neuronal identity
CC (PubMed:1842358). As a transcriptional factor, regulates the expression
CC of ftz, eve and en in a subset of neuroblast progeny and modulates the
CC transcriptional activity of other homeodomain proteins such as Dfd
CC (PubMed:12429095, PubMed:15837198, PubMed:9380747). Required for proper
CC neuronal differentiation, axonal outgrowth and pathfinding of most or
CC all neurons and their precursors in central and peripheral nervous
CC systems (PubMed:20152183, PubMed:27510969, PubMed:1720353,
CC PubMed:1842358, PubMed:1540176, PubMed:11051550, PubMed:18342578,
CC PubMed:23056424, PubMed:16564014). Regulates asymmetric stem cell self-
CC renewal together with brat (PubMed:16564014).
CC {ECO:0000269|PubMed:11051550, ECO:0000269|PubMed:12429095,
CC ECO:0000269|PubMed:1540176, ECO:0000269|PubMed:15837198,
CC ECO:0000269|PubMed:16564014, ECO:0000269|PubMed:1720353,
CC ECO:0000269|PubMed:1842358, ECO:0000269|PubMed:20152183,
CC ECO:0000269|PubMed:27510969, ECO:0000269|PubMed:9380747}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1540176,
CC ECO:0000269|PubMed:1720353, ECO:0000269|PubMed:18342578,
CC ECO:0000269|PubMed:1842358, ECO:0000269|PubMed:20152183,
CC ECO:0000269|PubMed:23056424}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:16564014, ECO:0000269|PubMed:23056424}. Cytoplasm
CC {ECO:0000269|PubMed:18342578}. Note=Dispersed in the cytoplasm of
CC mitotic ganglion mother cells (GMCs) and neuroblast-like cell type.
CC {ECO:0000269|PubMed:18342578}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=M {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-1; Sequence=Displayed;
CC Name=I {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-2; Sequence=VSP_002307, VSP_002308;
CC Name=H {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-4; Sequence=VSP_002308;
CC Name=J {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-5; Sequence=VSP_059598;
CC Name=L {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-6; Sequence=VSP_059598, VSP_002308;
CC Name=K {ECO:0000312|FlyBase:FBgn0004595};
CC IsoId=P29617-7; Sequence=VSP_059598, VSP_002307, VSP_002308;
CC -!- DEVELOPMENTAL STAGE: In embryos, expressed mainly in neuronal
CC precursors but detected also in cells lining the gut and Garland cells
CC (at protein level) (PubMed:20152183, PubMed:27510969, PubMed:1720353,
CC PubMed:1540176). Expressed during embryogenesis in different cells of
CC the central and peripheral nervous system including neuroblasts and
CC ganglion mother cells (GMC) (PubMed:1720353, PubMed:1842358).
CC {ECO:0000269|PubMed:1540176, ECO:0000269|PubMed:1720353,
CC ECO:0000269|PubMed:1842358, ECO:0000269|PubMed:20152183,
CC ECO:0000269|PubMed:27510969}.
CC -!- DOMAIN: The Prospero-type homeodomain and the adjacent Prospero domain
CC act as a single structural unit, the Homeo-Prospero domain.
CC {ECO:0000255|PROSITE-ProRule:PRU01162, ECO:0000269|PubMed:12429095,
CC ECO:0000269|PubMed:15837198}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal, results in the aberrant
CC expression of the homeobox genes ftz, eve and en in a subset of
CC neuroblasts, and in axonal outgrowth and pathfinding defects in both
CC motoneurons and sensory neurons. {ECO:0000269|PubMed:1720353,
CC ECO:0000269|PubMed:1842358}.
CC -!- SIMILARITY: Belongs to the Prospero homeodomain family.
CC {ECO:0000255|PROSITE-ProRule:PRU01162}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28841.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAL28228.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAL28228.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAT94492.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAA01464.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA77802.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M81389; AAA28841.1; ALT_FRAME; mRNA.
DR EMBL; AE014297; AAF54628.3; -; Genomic_DNA.
DR EMBL; AE014297; AAN13500.3; -; Genomic_DNA.
DR EMBL; AE014297; AAN13501.3; -; Genomic_DNA.
DR EMBL; AE014297; AFH06364.1; -; Genomic_DNA.
DR EMBL; Z11743; CAA77802.1; ALT_INIT; mRNA.
DR EMBL; D10609; BAA01464.1; ALT_INIT; mRNA.
DR EMBL; BT015263; AAT94492.1; ALT_INIT; mRNA.
DR EMBL; AF190403; AAF05703.1; -; Genomic_DNA.
DR EMBL; AY060680; AAL28228.1; ALT_SEQ; mRNA.
DR PIR; S24548; S24548.
DR RefSeq; NP_001247046.1; NM_001260117.2. [P29617-1]
DR RefSeq; NP_524317.4; NM_079593.6. [P29617-5]
DR RefSeq; NP_731565.4; NM_169386.3. [P29617-7]
DR RefSeq; NP_788636.3; NM_176459.4. [P29617-6]
DR PDB; 1MIJ; X-ray; 2.05 A; A=1545-1696.
DR PDB; 1XPX; X-ray; 2.80 A; A=1541-1703.
DR PDBsum; 1MIJ; -.
DR PDBsum; 1XPX; -.
DR AlphaFoldDB; P29617; -.
DR SMR; P29617; -.
DR BioGRID; 66496; 35.
DR IntAct; P29617; 6.
DR iPTMnet; P29617; -.
DR PaxDb; P29617; -.
DR EnsemblMetazoa; FBtr0304603; FBpp0293145; FBgn0004595. [P29617-4]
DR EnsemblMetazoa; FBtr0304604; FBpp0293146; FBgn0004595. [P29617-2]
DR EnsemblMetazoa; FBtr0304605; FBpp0293147; FBgn0004595. [P29617-5]
DR EnsemblMetazoa; FBtr0304606; FBpp0293148; FBgn0004595. [P29617-7]
DR EnsemblMetazoa; FBtr0304607; FBpp0293149; FBgn0004595. [P29617-6]
DR EnsemblMetazoa; FBtr0304608; FBpp0293150; FBgn0004595. [P29617-1]
DR GeneID; 41363; -.
DR KEGG; dme:Dmel_CG17228; -.
DR CTD; 41363; -.
DR FlyBase; FBgn0004595; pros.
DR VEuPathDB; VectorBase:FBgn0004595; -.
DR eggNOG; KOG3779; Eukaryota.
DR GeneTree; ENSGT00940000154790; -.
DR InParanoid; P29617; -.
DR OMA; CIETKRE; -.
DR SignaLink; P29617; -.
DR BioGRID-ORCS; 41363; 0 hits in 3 CRISPR screens.
DR ChiTaRS; pros; fly.
DR EvolutionaryTrace; P29617; -.
DR GenomeRNAi; 41363; -.
DR PRO; PR:P29617; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004595; Expressed in brain and 84 other tissues.
DR ExpressionAtlas; P29617; baseline and differential.
DR Genevisible; P29617; DM.
DR GO; GO:0045179; C:apical cortex; IDA:FlyBase.
DR GO; GO:0045180; C:basal cortex; IDA:FlyBase.
DR GO; GO:0005938; C:cell cortex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IMP:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0055059; P:asymmetric neuroblast division; IMP:FlyBase.
DR GO; GO:0055060; P:asymmetric neuroblast division resulting in ganglion mother cell formation; IMP:FlyBase.
DR GO; GO:0061564; P:axon development; IMP:UniProtKB.
DR GO; GO:0007411; P:axon guidance; IMP:FlyBase.
DR GO; GO:0007409; P:axonogenesis; IMP:FlyBase.
DR GO; GO:0060385; P:axonogenesis involved in innervation; IMP:FlyBase.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0043697; P:cell dedifferentiation; IGI:UniProtKB.
DR GO; GO:0045165; P:cell fate commitment; IMP:FlyBase.
DR GO; GO:0007417; P:central nervous system development; IMP:UniProtKB.
DR GO; GO:0070983; P:dendrite guidance; IMP:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0070314; P:G1 to G0 transition; IMP:FlyBase.
DR GO; GO:0007402; P:ganglion mother cell fate determination; IMP:FlyBase.
DR GO; GO:0010001; P:glial cell differentiation; IMP:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR GO; GO:0050771; P:negative regulation of axonogenesis; IMP:FlyBase.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:FlyBase.
DR GO; GO:0007406; P:negative regulation of neuroblast proliferation; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IMP:FlyBase.
DR GO; GO:0014016; P:neuroblast differentiation; IMP:UniProtKB.
DR GO; GO:0007405; P:neuroblast proliferation; IMP:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IGI:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0008104; P:protein localization; IMP:FlyBase.
DR GO; GO:0007465; P:R7 cell fate commitment; IMP:FlyBase.
DR GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:1902692; P:regulation of neuroblast proliferation; IGI:UniProtKB.
DR GO; GO:0045664; P:regulation of neuron differentiation; IMP:UniProtKB.
DR GO; GO:1900180; P:regulation of protein localization to nucleus; IMP:FlyBase.
DR GO; GO:0045676; P:regulation of R7 cell differentiation; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050909; P:sensory perception of taste; IMP:FlyBase.
DR GO; GO:0007416; P:synapse assembly; IMP:FlyBase.
DR Gene3D; 1.10.10.500; -; 1.
DR InterPro; IPR023082; Homeo_prospero_dom.
DR InterPro; IPR037131; Homeo_prospero_dom_sf.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR039350; Prospero_homeodomain.
DR PANTHER; PTHR12198; PTHR12198; 2.
DR Pfam; PF05044; HPD; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51818; HOMEO_PROSPERO; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Developmental protein;
KW DNA-binding; Homeobox; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1703
FT /note="Homeobox protein prospero"
FT /id="PRO_0000208884"
FT DOMAIN 1545..1603
FT /note="Prospero-type homeo"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT DOMAIN 1604..1703
FT /note="Prospero"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT REGION 23..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 322..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 437..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 608..692
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..751
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 823..844
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 875..951
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1040..1101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1197..1217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1245..1389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1418..1469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1545..1703
FT /note="Homeo-Prospero"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT MOTIF 1291..1298
FT /note="Nuclear localization signal"
FT COMPBIAS 23..109
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..126
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..259
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 437..475
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 501..521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..645
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 646..671
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 897..912
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 926..951
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1040..1057
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1058..1079
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1246..1285
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1327..1369
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1372..1388
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 1582
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1587
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1590
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1591
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1594
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1597
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT SITE 1676
FT /note="Interaction with DNA"
FT /evidence="ECO:0000269|PubMed:12429095,
FT ECO:0000269|PubMed:15837198, ECO:0007744|PDB:1MIJ,
FT ECO:0007744|PDB:1XPX"
FT MOD_RES 479
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 482
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 485
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 497
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 651
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 654
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..300
FT /note="Missing (in isoform J, isoform L and isoform K)"
FT /evidence="ECO:0000305"
FT /id="VSP_059598"
FT VAR_SEQ 1216
FT /note="S -> SGNNGSLLLANSQMPSQTTASGSSAQQQQQQNAQQQHQGSQQQQQQN
FT VVQQQNVAQQQHMQQQQQQQQSHPLLPPNCQQLISAPRLNGSQLSFASPAAAAAAAMGL
FT QMHHAAAAAAMSAQQQQQQSGDPGANTNPNSGPANPTNSSLSTLNIPPPHIRPSPT
FT (in isoform I and isoform K)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_002307"
FT VAR_SEQ 1516..1544
FT /note="Missing (in isoform I, isoform H, isoform L and
FT isoform K)"
FT /evidence="ECO:0000303|PubMed:12537569, ECO:0000303|Ref.6"
FT /id="VSP_002308"
FT CONFLICT 49..57
FT /note="QQQQQQHHH -> HHQ (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 124..135
FT /note="ANEEESEDSDDD -> RTKRSPRIPTTT (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="A -> G (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="S -> L (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="S -> R (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="A -> R (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 376..398
FT /note="AKMLNELFGRQMKQAQDATSGLP -> GQDAERAVWPPDEAGPGRNEWPA
FT (in Ref. 1; AAA28841 and 7; AAF05703)"
FT /evidence="ECO:0000305"
FT CONFLICT 420..444
FT /note="IGSLNSTSKLLQQQHNNNSIAPANS -> NLALQFHVQVAAAAAITTALLPP
FT IG (in Ref. 1; AAA28841 and 7; AAF05703)"
FT /evidence="ECO:0000305"
FT CONFLICT 718
FT /note="H -> Q (in Ref. 5; BAA01464)"
FT /evidence="ECO:0000305"
FT CONFLICT 977
FT /note="A -> C (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 1102
FT /note="A -> S (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 1258
FT /note="T -> S (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT CONFLICT 1348
FT /note="Q -> QQQQQ (in Ref. 1; AAA28841)"
FT /evidence="ECO:0000305"
FT HELIX 1550..1559
FT /evidence="ECO:0007829|PDB:1MIJ"
FT TURN 1560..1562
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1568..1574
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1582..1612
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1628..1637
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1647..1665
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1668..1670
FT /evidence="ECO:0007829|PDB:1MIJ"
FT TURN 1672..1675
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1676..1683
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1691..1694
FT /evidence="ECO:0007829|PDB:1MIJ"
FT HELIX 1696..1699
FT /evidence="ECO:0007829|PDB:1XPX"
SQ SEQUENCE 1703 AA; 184980 MW; 35040F15C1CBB1D8 CRC64;
MMSSEEYEAD CFGLYSDENN VLLKANEPET TAATKQQHQP QFQQQQQQQQ QQQQHHHQHQ
QPPPSSNGHT SPIPSQVNGD GSAANCGESG KTNTNTGSHS HSNSGNTNTD ADKEQEREKA
KEKANEEESE DSDDDVVVVL EGCEGNASSS SSSSNSNSNA SSNNHKAAAT TTTTATTANH
CNRSGGSSRS HRSARSSRQI SQSTAVGKTT TCAAKKPTAA QATTTTAKNS NSNSNVNVNV
NVNSNGSGNG NANSKVNRRS RQRSLSKDIN NQPASSNSNS NSSNNSSNSN GGATATAAGF
MSSAAAAAAG AAGGGALFQP QSVSTANSSS SNNNNSSTPA ALATHSPTSN SPVSGASSAS
SLLTAAFGNL FGGSSAKMLN ELFGRQMKQA QDATSGLPQS LDNAMLAAAM ETATSAELLI
GSLNSTSKLL QQQHNNNSIA PANSTPMSNG TNASISPGSA HSSSHSHQGV SPKGSRRVSA
CSDRSLEAAA ADVAGGSPPR AASVSSLNGG ASSGEQHQSQ LQHDLVAHHM LRNILQGKKE
LMQLDQELRT AMQQQQQQLQ EKEQLHSKLN NNNNNNIAAT ANNNNNTTME SINLIDDSEM
ADIKIKSEPQ TAPQPQQSPH GSSHSSRSGS GSGSHSSMAS DGSLRRKSSD SLDSHGAQDD
AQDEEDAAPT GQRSESRAPE EPQLPTKKES VDDMLDEVEL LGLHSRGSDM DSLASPSHSD
MMLLDKDDVL DEDDDDDCVE QKTSGSGCLK KPGMDLKRAR VENIVSGMRC SPSSGLAQAG
QLQVNGCKKR KLYQPQQHAM ERYVAAAAGL NFGLNLQSMM LDQEDSESNE LESPQIQQKR
VEKNALKSQL RSMQEQLAEM QQKYVQLCSR MEQESECQEL DQDQDVEQEQ EPDNGSSDHI
ELSPSPTLTG DGDVSPNHKE ETGQERPGSS SPSPSPLKPK TSLGESSDSG ANMLSQMMSK
MMSGKLHNPL VGVGHPALPQ GFPPLLQHMG DMSHAAAMYQ QFFFEQEARM AKEAAEQQQQ
QQQQQQQQQQ QQQQEQQRRF EQEQQEQQRR KEEQQQQIQR QQQHLQQLQQ QQMEQQHVAT
AAPRPQMHHP APARLPTRMG GAAGHTALKS ELSEKFQMLR ANNNSSMMRM SGTDLEGLAD
VLKSEITTSL SALVDTIVTR FVHQRRLFSK QADSVTAAAE QLNKDLLLAS QILDRKSPRT
KVADRPQNGP TPATQSAAAM FQAPKTPQGM NPVAAAALYN SMTGPFCLPP DQQQQQQTAQ
QQQSAQQQQQ SSQQTQQQLE QNEALSLVVT PKKKRHKVTD TRITPRTVSR ILAQDGVVPP
TGGPPSTPQQ QQQQQQQQQQ QQQQQQQQAS NGGNSNATPA QSPTRSSGGA AYHPQPPPPP
PPMMPVSLPT SVAIPNPSLH ESKVFSPYSP FFNPHAAAGQ ATAAQLHQHH QQHHPHHQSM
QLSSSPPGSL GALMDSRDSP PLPHPPSMLH PALLAAAHHG GSPDYKTCLR AVMDAQDRQS
ECNSADMQFD GMAPTISFYK QMQLKTEHQE SLMAKHCESL TPLHSSTLTP MHLRKAKLMF
FWVRYPSSAV LKMYFPDIKF NKNNTAQLVK WFSNFREFYY IQMEKYARQA VTEGIKTPDD
LLIAGDSELY RVLNLHYNRN NHIEVPQNFR FVVESTLREF FRAIQGGKDT EQSWKKSIYK
IISRMDDPVP EYFKSPNFLE QLE
