PROS1_CAEEL
ID PROS1_CAEEL Reviewed; 594 AA.
AC P34522;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Homeobox protein prospero homolog 1 {ECO:0000312|WormBase:K12H4.1};
DE AltName: Full=Homeobox protein ceh-26 {ECO:0000305};
GN Name=pros-1 {ECO:0000312|WormBase:K12H4.1};
GN Synonyms=ceh-26 {ECO:0000312|WormBase:K12H4.1};
GN ORFNames=K12H4.1 {ECO:0000312|WormBase:K12H4.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP SIMILARITY TO DROSOPHILA PROSPERO.
RX PubMed=7909177; DOI=10.1016/0968-0004(94)90035-3;
RA Buerglin T.R.;
RT "A Caenorhabditis elegans prospero homologue defines a novel domain.";
RL Trends Biochem. Sci. 19:70-71(1994).
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23334499; DOI=10.1038/ncb2662;
RA Kolotuev I., Hyenne V., Schwab Y., Rodriguez D., Labouesse M.;
RT "A pathway for unicellular tube extension depending on the lymphatic vessel
RT determinant Prox1 and on osmoregulation.";
RL Nat. Cell Biol. 15:157-168(2013).
CC -!- FUNCTION: Transcription factor involved in developmental processes (By
CC similarity). Controls the transcription of genes required for excretory
CC canal formation (PubMed:23334499). {ECO:0000250|UniProtKB:Q92786,
CC ECO:0000269|PubMed:23334499}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the excretory cell at the 1.5-fold
CC embryonic stage and throughout larval development.
CC {ECO:0000269|PubMed:23334499}.
CC -!- DOMAIN: The Prospero-type homeodomain and the adjacent Prospero domain
CC act as a single structural unit, the Homeo-Prospero domain.
CC {ECO:0000255|PROSITE-ProRule:PRU01162}.
CC -!- DISRUPTION PHENOTYPE: Lethality at larval stage, L1. Surviving animals
CC do not develop into fertile adults. Animals have abnormal sensory
CC function with their bodies harboring lesions and signs of progressive
CC degradation. Defective excretory canal formation with down-regulation
CC of genes, including exc-5, ifb-1, gck-3 and aqp-8, involved in this
CC process. Seventy percent of L1 larvae do not form excretory canals, but
CC have a large vacuole around the excretory cell body. The remaining
CC larvae form impaired excretory canals that rarely extend beyond the
CC pharyngeal-intestinal valve and do not extend to the somatic gonad.
CC Furthermore, some animals develop a cyst in the lumen of the excretory
CC canal. RNAi-mediated knockdown in larvae largely results in arrest at
CC the L1 larval stage. Surviving worms have altered excretory canal
CC growth with a large vacuole and cyst in the excretory cell body and
CC lumen, respectively, and the formation of a thin canal extension.
CC {ECO:0000269|PubMed:23334499}.
CC -!- SIMILARITY: Belongs to the Prospero homeodomain family.
CC {ECO:0000255|PROSITE-ProRule:PRU01162}.
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DR EMBL; FO081380; CCD71202.1; -; Genomic_DNA.
DR PIR; S44850; S44850.
DR RefSeq; NP_498760.2; NM_066359.4.
DR AlphaFoldDB; P34522; -.
DR SMR; P34522; -.
DR BioGRID; 41343; 4.
DR IntAct; P34522; 1.
DR MINT; P34522; -.
DR STRING; 6239.K12H4.1; -.
DR EPD; P34522; -.
DR PaxDb; P34522; -.
DR PeptideAtlas; P34522; -.
DR EnsemblMetazoa; K12H4.1.1; K12H4.1.1; WBGene00000448.
DR GeneID; 176137; -.
DR KEGG; cel:CELE_K12H4.1; -.
DR UCSC; K12H4.1; c. elegans.
DR CTD; 176137; -.
DR WormBase; K12H4.1; CE45017; WBGene00000448; pros-1.
DR eggNOG; KOG3779; Eukaryota.
DR GeneTree; ENSGT00940000154790; -.
DR HOGENOM; CLU_443616_0_0_1; -.
DR InParanoid; P34522; -.
DR OMA; PDFFKEP; -.
DR OrthoDB; 130558at2759; -.
DR PRO; PR:P34522; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000448; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0021782; P:glial cell development; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:WormBase.
DR Gene3D; 1.10.10.500; -; 1.
DR InterPro; IPR023082; Homeo_prospero_dom.
DR InterPro; IPR037131; Homeo_prospero_dom_sf.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR039350; Prospero_homeodomain.
DR PANTHER; PTHR12198; PTHR12198; 3.
DR Pfam; PF05044; HPD; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51818; HOMEO_PROSPERO; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..594
FT /note="Homeobox protein prospero homolog 1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000208883"
FT DOMAIN 435..493
FT /note="Prospero-type homeo"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT DOMAIN 494..593
FT /note="Prospero"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 30..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 435..593
FT /note="Homeo-Prospero"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01162"
FT COMPBIAS 34..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..161
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..378
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 594 AA; 66518 MW; 5B2027ED2FC93EF0 CRC64;
MSSGLPSIAA TAQPSANGFS FNNGFPPFGI YYSQLPPVNS NGSTPNSSNR FKVKRHRQRV
DAGEPRNTYQ GASTSVSSNS SSSSSTSNTN STPSSSSTSS KKSTEGMTET ETMTASIEQE
KVIQNEESEA GKDGMEEHDD GMNDFEIIDD TNDEVEESEE RESPESNSSS ASKRKSFQPQ
KIGEELGIEF GDETEQINVT VGGTEEAEET TDEAEGRELS ALQAQLQNGH IAEQQRRFYA
AFLDQQRKQV AAAQILHNGK INIERLVSSV KGDLVTNFMK DLEKVIRDWA ADEVLKQEAA
APPQIPQPPP QFHPIFPANP LFHMAPPHHP VAGGGIFPPN FNAFNAFNAL RRNLQDVDTS
SEMKKKRTKV EIKKEDAMSS RASPLSASAS PPLSRFFPAP TMVGHYGGMN FGDREDSPTN
SDELSECGYE GGGSSSMLTP MHLRKAKLMF FYTRYPNSNL LKSYFPDIRF NKNNTAQLVK
WFSNFREFYY NQMEKFARQA LAEGITDRND IFVSKDSELF KVLNTHYNRN NHIKAPDRLV
FVVQETLREF HDAIKQGKDI EPSWKKTIYK VINRLEDQIP DFFKEPNFLE RLET
