ID   PROF_VACCW              Reviewed;         133 AA.
AC   Q76ZN5; P20844;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   23-FEB-2022, entry version 73.
DE   RecName: Full=Profilin;
GN   OrderedLocusNames=VACWR167; ORFNames=A42R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1870190; DOI=10.1128/jvi.65.9.4598-4608.1991;
RA   Blasco R., Cole N.B., Moss B.;
RT   "Sequence analysis, expression, and deletion of a vaccinia virus gene
RT   encoding a homolog of profilin, a eukaryotic actin-binding protein.";
RL   J. Virol. 65:4598-4608(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   PubMed=8075084; DOI=10.1021/bi00201a032;
RA   Machesky L.M., Cole N.B., Moss B., Pollard T.D.;
RT   "Vaccinia virus expresses a novel profilin with a higher affinity for
RT   polyphosphoinositides than actin.";
RL   Biochemistry 33:10815-10824(1994).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=16509968; DOI=10.1186/1743-422x-3-10;
RA   Yoder J.D., Chen T.S., Gagnier C.R., Vemulapalli S., Maier C.S.,
RA   Hruby D.E.;
RT   "Pox proteomics: mass spectrometry analysis and identification of Vaccinia
RT   virion proteins.";
RL   Virol. J. 3:10-10(2006).
CC   -!- FUNCTION: Participates in either intracellular transport of viral
CC       proteins or intercellular spread of the virus. Cellular profilins
CC       modulate actin filament dynamics (polymerization and depolymerization)
CC       via direct binding to actin through an actin-binding domain as well as
CC       by modulation of other actin-binding proteins (By similarity). In
CC       contrast to cellular homologs, the poxvirus profilins seem to bind
CC       actin only weakly. {ECO:0000250, ECO:0000269|PubMed:8075084}.
CC   -!- SUBUNIT: Interacts with host TPM1. Interacts with protein A25 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm.
CC   -!- SIMILARITY: Belongs to the profilin family. {ECO:0000305}.
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DR   EMBL; M72474; AAA48308.1; -; Genomic_DNA.
DR   EMBL; D11079; BAA01815.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89446.1; -; Genomic_DNA.
DR   PIR; B40897; FAVZVR.
DR   RefSeq; YP_233049.1; NC_006998.1.
DR   SMR; Q76ZN5; -.
DR   IntAct; Q76ZN5; 1.
DR   MINT; Q76ZN5; -.
DR   DNASU; 3707697; -.
DR   GeneID; 3707697; -.
DR   KEGG; vg:3707697; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0039680; P:actin-dependent intracellular transport of virus towards nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR005455; PFN.
DR   InterPro; IPR036140; PFN_sf.
DR   InterPro; IPR027310; Profilin_CS.
DR   Pfam; PF00235; Profilin; 1.
DR   SMART; SM00392; PROF; 1.
DR   SUPFAM; SSF55770; SSF55770; 1.
DR   PROSITE; PS00414; PROFILIN; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Actin-dependent inwards viral transport;
KW   Cytoplasmic inwards viral transport; Host cytoplasm;
KW   Host-virus interaction; Reference proteome; Virus entry into host cell.
FT   CHAIN           1..133
FT                   /note="Profilin"
FT                   /id="PRO_0000199683"
SQ   SEQUENCE   133 AA;  15052 MW;  C76E5D9FA31E8669 CRC64;
     MAEWHKIIED ISKNNKFEDA AIVDYKTTKN VLAAIPNRTF AKINPGEIIP LITNRNILKP
     LIGQKYCIVY TNSLMDENTY AMELLTGYAP VSPIVIARTH TALIFLMGKP TTSRRDVYRT
     CRDHATRVRA TGN