ID   PRIA_RICBR              Reviewed;         648 AA.
AC   Q1RI08;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=RBE_0925;
OS   Rickettsia bellii (strain RML369-C).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; belli group.
OX   NCBI_TaxID=336407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RML369-C;
RX   PubMed=16703114; DOI=10.1371/journal.pgen.0020076;
RA   Ogata H., La Scola B., Audic S., Renesto P., Blanc G., Robert C.,
RA   Fournier P.-E., Claverie J.-M., Raoult D.;
RT   "Genome sequence of Rickettsia bellii illuminates the role of amoebae in
RT   gene exchanges between intracellular pathogens.";
RL   PLoS Genet. 2:733-744(2006).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
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DR   EMBL; CP000087; ABE05006.1; -; Genomic_DNA.
DR   RefSeq; WP_011477587.1; NC_007940.1.
DR   AlphaFoldDB; Q1RI08; -.
DR   SMR; Q1RI08; -.
DR   STRING; 336407.RBE_0925; -.
DR   EnsemblBacteria; ABE05006; ABE05006; RBE_0925.
DR   KEGG; rbe:RBE_0925; -.
DR   eggNOG; COG1198; Bacteria.
DR   HOGENOM; CLU_013353_4_0_5; -.
DR   OMA; RCHYCGY; -.
DR   OrthoDB; 1132322at2; -.
DR   Proteomes; UP000001951; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Zinc; Zinc-finger.
FT   CHAIN           1..648
FT                   /note="Primosomal protein N'"
FT                   /id="PRO_0000281069"
FT   DOMAIN          131..297
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   DOMAIN          393..548
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         358..370
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         385..401
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   MOTIF           240..243
FT                   /note="DEAH box"
FT   BINDING         144..151
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   648 AA;  73480 MW;  938DCF7E8739303A CRC64;
     MQIAKILLSA VKLFPLDYLI PEDLSLNIGD LVVVPFRNKE LTGIVWEFSN VPEVKKLKTI
     KEKVPLNLTI TSEVLDLIKW MSNYYMAELG SIAKLVLPVD IAEKPIKVKE QKINNNFTLP
     DLSEEQKQAL QMLNDSNKPI LIKGVTGSGK TEVYFYLIAE YLAKGKQALV MLPEIALSTQ
     IINRFKERFG FDPIIWNSSV TKAQKKMILR GILEDKVKIV IGARSSLFLP FKNLGLIVID
     EEHDDSYKQD DGVLYNARDT AIVRSTFDKA QIVLCSATPS IETMYNANIG KYSVINLSSR
     YKNAVLPNIE MIDMTKEELP KNCYLSNILI KAIKNNLENK EQTLLFLNRR GYAPLMLCKS
     CGHKFTCKYC SSWMVVHKSS KKLECHHCGY QSKIFNSCPE CLEDESLAIC GPGIERIEEE
     AKTLFPNSKI AVISKDHVKN PEKIAQLLHQ MENLEIDILI GTQMITKGYH FPNLTLVGVV
     DADLGSNHAD LRSSERAFQL LHQVGGRAGR GNSKGMVYLQ SYYPDNIIFS YIKDEREDNF
     FVNELEVRKA ADMPPFSKMA SVILSGTNEH KILEVAKNMV RIAPEAQVKI LGPASSLMSK
     LAGKYRFRIL IIADKKFNLQ KYLKFWLELI KIPSFCHIKI DIDPKSFY