PPT3_ORYSJ
ID PPT3_ORYSJ Reviewed; 393 AA.
AC Q5VQL3; A0A0P0UYM6;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Phosphoenolpyruvate/phosphate translocator 3, chloroplastic;
DE Short=OsPPT3;
DE Flags: Precursor;
GN Name=PPT3; OrderedLocusNames=Os01g0172100, LOC_Os01g07730;
GN ORFNames=P0583G08.29;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION.
RX PubMed=17981875; DOI=10.1093/pcp/pcm150;
RA Nozawa A., Nanamiya H., Miyata T., Linka N., Endo Y., Weber A.P.,
RA Tozawa Y.;
RT "A cell-free translation and proteoliposome reconstitution system for
RT functional analysis of plant solute transporters.";
RL Plant Cell Physiol. 48:1815-1820(2007).
CC -!- FUNCTION: Phosphoenolpyruvate/phosphate translocator that transports
CC phosphoenolpyruvate (PEP) and dihydroxyacetone phosphate.
CC {ECO:0000269|PubMed:17981875}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. PPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; AP003282; BAD68262.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04064.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70631.1; -; Genomic_DNA.
DR EMBL; AK060343; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015621423.1; XM_015765937.1.
DR RefSeq; XP_015621424.1; XM_015765938.1.
DR AlphaFoldDB; Q5VQL3; -.
DR SMR; Q5VQL3; -.
DR STRING; 4530.OS01T0172100-01; -.
DR PaxDb; Q5VQL3; -.
DR PRIDE; Q5VQL3; -.
DR EnsemblPlants; Os01t0172100-01; Os01t0172100-01; Os01g0172100.
DR GeneID; 4327644; -.
DR Gramene; Os01t0172100-01; Os01t0172100-01; Os01g0172100.
DR KEGG; osa:4327644; -.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_019048_0_0_1; -.
DR InParanoid; Q5VQL3; -.
DR OMA; MFATWYL; -.
DR OrthoDB; 1453018at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5VQL3; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015121; F:phosphoenolpyruvate:phosphate antiporter activity; IDA:UniProtKB.
DR GO; GO:0071917; F:triose-phosphate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015714; P:phosphoenolpyruvate transport; IDA:UniProtKB.
DR GO; GO:0035436; P:triose phosphate transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR InterPro; IPR004696; Tpt_PEP_transl.
DR Pfam; PF03151; TPT; 1.
DR TIGRFAMs; TIGR00817; tpt; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Pyruvate; Reference proteome;
KW Sugar transport; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 66..393
FT /note="Phosphoenolpyruvate/phosphate translocator 3,
FT chloroplastic"
FT /id="PRO_0000406103"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 123..228
FT /note="EamA"
FT CONFLICT 31
FT /note="G -> D (in Ref. 5; AK060343)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 393 AA; 41735 MW; 24FB790A79D9900E CRC64;
MQRAAAASRA TAWSTARHGA ARVTASASFS GGGGIVAGAA LPLRVRGGQL MSLPLLSGGR
AVTARVAAAE APLPADDADA AAGRERGALA ETAQLGAMIV AWYLLNIYFN IYNKQVLQPL
PFPYTITAFQ LAFGSFVIFL MWALKLHPAP RISISQLAKI APLAAGHMLG TVFTNMSLSK
VAVSFTHTIK ASEPFFTVLL SAFFLGETPS LLVLGSLVPI VGGVALASLT ELSFNWIGFW
SAMASNLLYQ SRNVLSKKLL GGEEEALDDI NLFSILTILS FLLSLPLMLF SEGVKFSPGY
LRSTGLNLQE LCVRAALAGF CFHGYQKLSY LILARVSPVT HSVANCVKRV VVIVASVLFF
RTPISPVNAL GTGVALGGVF LYSRLKRTKP KNA
