ID   PPR4_SCHPO              Reviewed;         931 AA.
AC   O14275;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Pentatricopeptide repeat-containing protein 4, mitochondrial;
DE   Flags: Precursor;
GN   Name=ppr4; ORFNames=SPAC8C9.06c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   DOMAIN, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=21727087; DOI=10.1093/nar/gkr511;
RA   Kuhl I., Dujeancourt L., Gaisne M., Herbert C.J., Bonnefoy N.;
RT   "A genome wide study in fission yeast reveals nine PPR proteins that
RT   regulate mitochondrial gene expression.";
RL   Nucleic Acids Res. 39:8029-8041(2011).
CC   -!- FUNCTION: Mitochondrial RNA-binding protein that specifically activates
CC       the translation of the cox1 mRNA. {ECO:0000269|PubMed:21727087}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:16823372,
CC       ECO:0000269|PubMed:21727087}.
CC   -!- DISRUPTION PHENOTYPE: Impairs growth on galactose.
CC       {ECO:0000269|PubMed:21727087}.
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DR   EMBL; CU329670; CAB16294.1; -; Genomic_DNA.
DR   PIR; T39143; T39143.
DR   RefSeq; NP_594277.1; NM_001019700.2.
DR   AlphaFoldDB; O14275; -.
DR   BioGRID; 279736; 1.
DR   STRING; 4896.SPAC8C9.06c.1; -.
DR   MaxQB; O14275; -.
DR   PaxDb; O14275; -.
DR   EnsemblFungi; SPAC8C9.06c.1; SPAC8C9.06c.1:pep; SPAC8C9.06c.
DR   GeneID; 2543312; -.
DR   KEGG; spo:SPAC8C9.06c; -.
DR   PomBase; SPAC8C9.06c; ppr4.
DR   VEuPathDB; FungiDB:SPAC8C9.06c; -.
DR   eggNOG; KOG4197; Eukaryota.
DR   HOGENOM; CLU_315967_0_0_1; -.
DR   InParanoid; O14275; -.
DR   OMA; SYCKQGA; -.
DR   PRO; PR:O14275; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0099616; C:extrinsic component of matrix side of mitochondrial inner membrane; ISO:PomBase.
DR   GO; GO:0005739; C:mitochondrion; IDA:PomBase.
DR   GO; GO:0045182; F:translation regulator activity; ISO:PomBase.
DR   GO; GO:0140053; P:mitochondrial gene expression; IMP:PomBase.
DR   GO; GO:0070134; P:positive regulation of mitochondrial translational initiation; ISO:PomBase.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 3.
DR   InterPro; IPR002885; Pentatricopeptide_repeat.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF01535; PPR; 1.
PE   4: Predicted;
KW   Activator; Mitochondrion; Protein biosynthesis; Reference proteome; Repeat;
KW   Transit peptide; Translation regulation.
FT   TRANSIT         1..16
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..931
FT                   /note="Pentatricopeptide repeat-containing protein 4,
FT                   mitochondrial"
FT                   /id="PRO_0000317702"
FT   REPEAT          247..277
FT                   /note="PPR 1"
FT   REPEAT          282..316
FT                   /note="PPR 2"
FT   REPEAT          429..461
FT                   /note="PPR 3"
FT   REPEAT          462..496
FT                   /note="PPR 4"
FT   REPEAT          497..531
FT                   /note="PPR 5"
FT   REPEAT          598..632
FT                   /note="PPR 6"
FT   REPEAT          683..713
FT                   /note="PPR 7"
SQ   SEQUENCE   931 AA;  107984 MW;  D46AA3D7E40B46ED CRC64;
     MSKSFAYRHI WCFWRFNTPL LWFPQPLKYW PAFQQSHTFN SMSVFKNDNA IANQTTVNES
     DVKRNVEKIN DIYECSNNTK SPCFPNSDSR IPLVNWKTST NPTLQVRAYM LDLLLPFFTL
     DLLPFLNKVC ECCNHMLLDN PKIKQDLPFA FCYLFSSYFS YKSSRQYTCS SEEISKVFRH
     LNRLGGSEYV LHLFAQIPKA LCKELTDVRT LNHPLRSIFT FCFKNISNPP NLLKLLTKLY
     PRSDRANEVL YTQYLGFLTK RGDYQIAIYM FDEMYRTHHW SSFTACRLMI ESLVRQNKFE
     EAISLYKKII AKRPKIARDK KILNLLLYIS TVSNRSPDAF KLALQSISNA NQIPSFDVFS
     RLMSALVKYN MTEMILPLVK QYDHKFRNLY SPNIFLSIVQ ALVFCGDMVN IQRWYNMSRV
     NSELSRIKHL LNCFLNSSTV SLDVSMVLEL LRDLKKKKIK VDERTLVICI TIFSRRKDLF
     AMEKIHQYFS DQGIKTSNQA YAALLDAYIE AEDTEKIELY LGKIRRLGIT EDVSINRMLM
     RLALDRLDWD LLEQCTKVAE RKDPEGQDYL STITMLYHVR QHELNLALRI FSGIQKPNVV
     HYSIAATVLG NLNQLDQLLL LEKRMESEGK APTALSLVAF VSSYCKQGAE GLDEAKRYMS
     KNFQPDKRAL LFNPRTANDM TYPPSLFSSL IKEYTSLGDI KEAKQVLSTY LEYFSKNITS
     KPDIPFAIAS MRLYCSLHDT VLSRQFWDLI LQVAQQNFIT TDIAAVVDDK NIPSPSGVIP
     AYKSALNVAA ETYFSFLASV NSFQELDQEW SRLEKLGFEY DDSLQNKRII WLLYDDRLDA
     AIKRVYNIFL SSKRIEELIP DASSQNSIIS FFLSPDSPLY FSTLKALRDK LDMAVEDDFV
     RISQGILVPT VKYLQTIRRD NEILFNLLAK V