PPR42_HUMAN
ID PPR42_HUMAN Reviewed; 309 AA.
AC Q7Z4L9; G3V118;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 42 {ECO:0000305};
DE AltName: Full=Leucine-rich repeat-containing protein 67 {ECO:0000305};
GN Name=PPP1R42 {ECO:0000312|HGNC:HGNC:33732}; Synonyms=LRRC67;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulates phosphatase activity of protein phosphatase 1 (PP1)
CC complexes in the testis. {ECO:0000250|UniProtKB:Q8R1Z4}.
CC -!- SUBUNIT: Interacts with PPP1CC isoform gamma-2; the interaction is
CC direct. Interacts with actin, dynein, KIF5B, KIFC1 and tubulin.
CC Associates with microtubules (By similarity).
CC {ECO:0000250|UniProtKB:Q8R1Z4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q8R1Z4}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000250|UniProtKB:Q8R1Z4}.
CC Note=Colocalizes with alpha tubulin to the manchette of developing
CC spermatids. Detected to nuclear rim in pachytene spermatocytes.
CC Detected at nuclear surface, opposite the acrosome in elongating
CC spermatids. Detected at the microtubule-organizing center (MTOC).
CC Localized to the centrosomal region of late-stage spermatids (By
CC similarity). {ECO:0000250|UniProtKB:Q8R1Z4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7Z4L9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z4L9-2; Sequence=VSP_032587, VSP_032588;
CC -!- PTM: Phosphorylated; in the testis. {ECO:0000250|UniProtKB:Q8R1Z4}.
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DR EMBL; AC110998; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471068; EAW86927.1; -; Genomic_DNA.
DR EMBL; BC055413; AAH55413.1; -; mRNA.
DR CCDS; CCDS34902.1; -. [Q7Z4L9-2]
DR RefSeq; NP_001013648.1; NM_001013626.3. [Q7Z4L9-2]
DR RefSeq; NP_001335492.1; NM_001348563.1.
DR AlphaFoldDB; Q7Z4L9; -.
DR SMR; Q7Z4L9; -.
DR BioGRID; 130328; 1.
DR IntAct; Q7Z4L9; 3.
DR MINT; Q7Z4L9; -.
DR STRING; 9606.ENSP00000315035; -.
DR iPTMnet; Q7Z4L9; -.
DR PhosphoSitePlus; Q7Z4L9; -.
DR BioMuta; PPP1R42; -.
DR DMDM; 172045913; -.
DR jPOST; Q7Z4L9; -.
DR PaxDb; Q7Z4L9; -.
DR PeptideAtlas; Q7Z4L9; -.
DR PRIDE; Q7Z4L9; -.
DR ProteomicsDB; 32229; -.
DR ProteomicsDB; 69209; -. [Q7Z4L9-1]
DR ProteomicsDB; 69210; -. [Q7Z4L9-2]
DR Antibodypedia; 12079; 93 antibodies from 19 providers.
DR DNASU; 286187; -.
DR Ensembl; ENST00000324682.5; ENSP00000315035.5; ENSG00000178125.15. [Q7Z4L9-2]
DR Ensembl; ENST00000522909.5; ENSP00000429721.1; ENSG00000178125.15. [Q7Z4L9-1]
DR GeneID; 286187; -.
DR KEGG; hsa:286187; -.
DR UCSC; uc003xxc.4; human. [Q7Z4L9-1]
DR UCSC; uc064nlc.1; human.
DR CTD; 286187; -.
DR DisGeNET; 286187; -.
DR GeneCards; PPP1R42; -.
DR HGNC; HGNC:33732; PPP1R42.
DR HPA; ENSG00000178125; Group enriched (fallopian tube, testis).
DR MIM; 617720; gene.
DR neXtProt; NX_Q7Z4L9; -.
DR OpenTargets; ENSG00000178125; -.
DR PharmGKB; PA162394588; -.
DR VEuPathDB; HostDB:ENSG00000178125; -.
DR eggNOG; KOG2769; Eukaryota.
DR GeneTree; ENSGT00940000158260; -.
DR HOGENOM; CLU_062444_0_0_1; -.
DR InParanoid; Q7Z4L9; -.
DR OMA; IGDISLC; -.
DR OrthoDB; 1267922at2759; -.
DR PhylomeDB; Q7Z4L9; -.
DR TreeFam; TF329227; -.
DR PathwayCommons; Q7Z4L9; -.
DR SignaLink; Q7Z4L9; -.
DR BioGRID-ORCS; 286187; 10 hits in 1037 CRISPR screens.
DR ChiTaRS; PPP1R42; human.
DR GenomeRNAi; 286187; -.
DR Pharos; Q7Z4L9; Tbio.
DR PRO; PR:Q7Z4L9; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q7Z4L9; protein.
DR Bgee; ENSG00000178125; Expressed in right uterine tube and 90 other tissues.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0002177; C:manchette; ISS:UniProtKB.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISS:UniProtKB.
DR GO; GO:0005815; C:microtubule organizing center; ISS:UniProtKB.
DR GO; GO:0000164; C:protein phosphatase type 1 complex; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; ISS:UniProtKB.
DR GO; GO:0070840; F:dynein complex binding; ISS:UniProtKB.
DR GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0015631; F:tubulin binding; ISS:UniProtKB.
DR GO; GO:0035307; P:positive regulation of protein dephosphorylation; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR025875; Leu-rich_rpt_4.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF12799; LRR_4; 1.
DR PROSITE; PS51450; LRR; 6.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Leucine-rich repeat;
KW Reference proteome; Repeat.
FT CHAIN 1..309
FT /note="Protein phosphatase 1 regulatory subunit 42"
FT /id="PRO_0000326175"
FT REPEAT 29..50
FT /note="LRR 1"
FT REPEAT 51..72
FT /note="LRR 2"
FT REPEAT 73..94
FT /note="LRR 3"
FT REPEAT 95..116
FT /note="LRR 4"
FT REPEAT 117..138
FT /note="LRR 5"
FT REPEAT 147..168
FT /note="LRR 6"
FT REPEAT 169..190
FT /note="LRR 7"
FT DOMAIN 204..242
FT /note="LRRCT"
FT VAR_SEQ 224..228
FT /note="EFLDG -> GTYLY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032587"
FT VAR_SEQ 229..309
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032588"
SQ SEQUENCE 309 AA; 35480 MW; EC1EAB07EF70E3CA CRC64;
MVRLTLDLIA RNSNLKPRKE ETISQCLKKI THINFSDKNI DAIEDLSLCK NLSVLYLYDN
CISQITNLNY ATNLTHLYLQ NNCISCIENL RSLKKLEKLY LGGNYIAVIE GLEGLGELRE
LHVENQRLPL GEKLLFDPRT LHSLAKSLCI LNISNNNIDD ITDLELLENL NQLIAVDNQL
LHVKDLEFLL NKLMKLWKID LNGNPVCLKP KYRDRLILVS KSLEFLDGKE IKNIERQFLM
NWKASKDAKK ISKKRSSKNE DASNSLISNF KTMHHIVPVY YPQVGKPKLA FFSEIQRYPV
NANASPESS
