AT4_ORYSJ
ID AT4_ORYSJ Reviewed; 439 AA.
AC Q5JNT2;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Acyl transferase 4 {ECO:0000305};
DE Short=OsAT4 {ECO:0000303|PubMed:23391577};
DE EC=2.3.1.- {ECO:0000305};
DE AltName: Full=p-coumaroyl-CoA monolignol transferase {ECO:0000305};
DE Short=OsPMT {ECO:0000303|PubMed:22267741};
GN Name=AT4 {ECO:0000303|PubMed:23391577};
GN Synonyms=PMT {ECO:0000303|PubMed:22267741};
GN OrderedLocusNames=Os01g0291500 {ECO:0000312|EMBL:BAS71658.1},
GN LOC_Os01g18744 {ECO:0000305};
GN ORFNames=OsJ_01392 {ECO:0000312|EMBL:EAZ11526.1},
GN P0706B05.17 {ECO:0000312|EMBL:BAD86875.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=22267741; DOI=10.1074/jbc.m111.284497;
RA Withers S., Lu F., Kim H., Zhu Y., Ralph J., Wilkerson C.G.;
RT "Identification of grass-specific enzyme that acylates monolignols with p-
RT coumarate.";
RL J. Biol. Chem. 287:8347-8355(2012).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23391577; DOI=10.1104/pp.112.208694;
RA Bartley L.E., Peck M.L., Kim S.R., Ebert B., Manisseri C., Chiniquy D.M.,
RA Sykes R., Gao L., Rautengarten C., Vega-Sanchez M.E., Benke P.I.,
RA Canlas P.E., Cao P., Brewer S., Lin F., Smith W.L., Zhang X.,
RA Keasling J.D., Jentoff R.E., Foster S.B., Zhou J., Ziebell A., An G.,
RA Scheller H.V., Ronald P.C.;
RT "Overexpression of a BAHD acyltransferase, OsAt10, alters rice cell wall
RT hydroxycinnamic acid content and saccharification.";
RL Plant Physiol. 161:1615-1633(2013).
CC -!- FUNCTION: Grass-specific monolignol p-coumaroyl transferase involved in
CC the biosynthesis of acylated monolignols or monolignol conjugates that
CC serve as monomer precursors of lignin. Can synthesize sinapyl p-
CC coumarate, p-coumaryl p-coumarate, sinapyl caffeate and p-coumaryl
CC caffeate in vitro. {ECO:0000269|PubMed:22267741}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=35 uM for sinapyl alcohol {ECO:0000269|PubMed:22267741};
CC KM=105 uM for p-coumaroyl-CoA {ECO:0000269|PubMed:22267741};
CC KM=141 uM for p-coumaryl alcohol {ECO:0000269|PubMed:22267741};
CC Vmax=10.8 nmol/sec/mg enzyme toward sinapyl alcohol
CC {ECO:0000269|PubMed:22267741};
CC Vmax=12.5 nmol/sec/mg enzyme toward p-coumaroyl-CoA
CC {ECO:0000269|PubMed:22267741};
CC Vmax=54.2 nmol/sec/mg enzyme toward p-coumaryl alcohol
CC {ECO:0000269|PubMed:22267741};
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; AP002482; BAD86875.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04705.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71658.1; -; Genomic_DNA.
DR EMBL; CM000138; EAZ11526.1; -; Genomic_DNA.
DR EMBL; AK060689; BAG87540.1; -; mRNA.
DR EMBL; AK070443; BAG91955.1; -; mRNA.
DR RefSeq; XP_015621300.1; XM_015765814.1.
DR AlphaFoldDB; Q5JNT2; -.
DR SMR; Q5JNT2; -.
DR STRING; 4530.OS01T0291500-01; -.
DR PaxDb; Q5JNT2; -.
DR PRIDE; Q5JNT2; -.
DR EnsemblPlants; Os01t0291500-01; Os01t0291500-01; Os01g0291500.
DR EnsemblPlants; Os01t0291500-02; Os01t0291500-02; Os01g0291500.
DR GeneID; 4325160; -.
DR Gramene; Os01t0291500-01; Os01t0291500-01; Os01g0291500.
DR Gramene; Os01t0291500-02; Os01t0291500-02; Os01g0291500.
DR KEGG; osa:4325160; -.
DR eggNOG; ENOG502QQHA; Eukaryota.
DR HOGENOM; CLU_014546_2_0_1; -.
DR InParanoid; Q5JNT2; -.
DR OMA; VHVVPIQ; -.
DR OrthoDB; 1130893at2759; -.
DR SABIO-RK; Q5JNT2; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0050734; F:hydroxycinnamoyltransferase activity; IEA:UniProt.
DR GO; GO:0016410; F:N-acyltransferase activity; IBA:GO_Central.
DR GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Lignin biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..439
FT /note="Acyl transferase 4"
FT /id="PRO_0000437775"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT ACT_SITE 382
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ SEQUENCE 439 AA; 46885 MW; EA2DD1CEF770D145 CRC64;
MGFAVVRTNR EFVRPSAATP PSSGELLELS IIDRVVGLRH LVRSLHIFSA AAPSGGDAKP
SPARVIKEAL GKALVDYYPF AGRFVDGGGG PGSARVECTG EGAWFVEAAA GCSLDDVNGL
DHPLMIPEDD LLPDAAPGVH PLDLPLMMQV TEFSCGGFVV GLISVHTMAD GLGAGQFINA
VGDYARGLDR PRVSPVWARE AIPSPPKLPP GPPPELKMFQ LRHVTADLSL DSINKAKSAY
FAATGHRCST FDVAIAKTWQ ARTRALRLPE PTSRVNLCFF ANTRHLMAGA AAWPAPAAGG
NGGNGFYGNC FYPVSVVAES GAVEAADVAG VVGMIREAKA RLPADFARWA VADFREDPYE
LSFTYDSLFV SDWTRLGFLE ADYGWGPPSH VIPFAYYPFM AVAIIGAPPV PKTGARIMTQ
CVEDDHLPAF KEEIKAFDK
